Abstract
The imidazole fungicides imazalil, prochloraz, and triflumizole and the triazole growth retardant paclobutrazol strongly enhanced the shoot-inducing effect of 6-benzyladenine in Spathiphyllum floribundum ‘Petite’ Schott. Numerous small shoots and shoot meristems appeared at the basal part of the plant. This effect was confirmed when such widely different cytokinins as zeatin, meta-topolin, and thidiazuron were combined with imazalil. Neither these fungicides nor paclobutrazol showed cytokinin effects on cytokinin-free medium. The number of roots per explant could be augmented using particular concentrations, depending on the fungicide used. The combination prochloraz and 6-benzyladenine had a similar effect on Anthurium andreanum, which suggests that Araceae are especially sensitive to this interaction.
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Abbreviations
- BA:
-
6-benzyladenine
- BMA:
-
basal medium Anthurium
- BMS:
-
basal medium Spathiphyllum
- f.m.:
-
fresh mass
- GA:
-
gibberellin
- GA3 :
-
gibberellic acid
- IMA:
-
imazalil
- mT:
-
6-(3-hydroxybenzyl)adenine
- NFT:
-
Nutrient Film Technique
- PAR:
-
Photosynthetic Active Radiation
- PBZ:
-
paclobutrazol
- PRO:
-
prochloraz
- TDZ:
-
thidiazuron
- TRI:
-
triflumizole
- Z:
-
zeatin
References
Fonnesbech M, Fonnesbech A (1979) In vitro propagation of Spathiphyllum. Scientia Hortic 10:21–25
Geier T (1990) Anthurium. In: Ammirato PV, Evans DR, Sharp WR, Bajaj YPS (eds) Handbook of plant cell culture, vol 5. pp 228–252
Grossman K (1992) Plant growth retardants: their mode of action and benefit for physiological research. In: Karssen CM, van Loon LC, Vreugdenhil D (eds) Progress in Plant Growth Regulation, pp 788-797
Hare PD, VanStaden J (1994) Inhibitory effect of thidiazuron on the activity of cytokinin oxidase isolated from soybean callus. Plant Cell Physiol 35(8):1121–1125
Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33: 105–119
Letham DS (1978) Cytokinins. In: Letham DS, Goodwin PB, Higgins TJV (eds) Phytohormones and related compounds—a comprehensive treatise. Volume I. Elsevier, North Holland, pp 205–263
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497
Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87
Rademacher W (1991) Biochemical effects of plant growth retardants. In: Gausman HW (ed) Plant biochemical regulators. Marcel Dekker, New York, pp 169–199
Strnad M (1995) Meta-Topolin, a new growth substance from poplar leaves (Populus x canadensis cv. Robusta). Planta, in press
Tomlin C (ed) (1994) The Pesticide Manual. 10th edition. The British Crop Protection Council and The Royal Society of Chemistry, 1341 pp
Werbrouck SPO, Debergh PC (1995) Imazalil enhances the shoot inducing effect of benzyladenine in Spathiphyllum floribundum Schott. J Plant Growth Regul 14:105–107
Werbrouck SPO, Strnad M, Van Onckelen HA, Debergh PC (1996) Meta-topolin, an alternative to benzyladenine in tissue culture? Physiol Plant (accepted)
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Werbrouck, S.P.O., Debergh, P.C. Imidazole fungicides and paclobutrazol enhance cytokinin-induced adventitious shoot proliferation in Araceae. J Plant Growth Regul 15, 81–85 (1996). https://doi.org/10.1007/BF00192936
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DOI: https://doi.org/10.1007/BF00192936