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Plant regeneration from protoplasts of Panax ginseng (C.A. Meyer) through somatic embryogenesis

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Summary

Protoplasts of Panax ginseng were isolated from embryos obtained from the 4-year old embryogenic cell line KCTC PCL 49031 which was derived from a zygotic embryo. High protoplast yields of 22–25 × 106 protoplast / g tissue were obtained following 5–6 h digestion with 2% Cellulysin, 1% Pectinase and 1% Macerasae in half strength Murashige and Skoog's medium containing 12% mannitol. A plating density of 1×105 protoplasts /ml was found optimal for protoplast culture. An initial division frequency of 10% was obtained in an agarosegelled defined medium. Myo-inositol (6%) was found to be the most suitable osmoticum. Somatic embryos were formed from protoplast derived embryogenic callus, which regenerated into plantlets.

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Abbreviations

NAA:

naphthaleneacetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

Kn:

kinetin

BA:

benzyladenine

GA3 :

gibberellic acid

MS:

Murashige and Skoog medium

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Authors and Affiliations

  1. Department of Physiological Botany, University of Uppsala, Box 540, S-751 21, Uppsala, Sweden

    Sarita Arya & Tage Eriksson

  2. Genetic Engineering Research Institute, KIST, Box 17, Taejon, Korea

    Jang R. Liu

Authors
  1. Sarita Arya
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  2. Jang R. Liu
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  3. Tage Eriksson
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Communicated by H. Lörz

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Arya, S., Liu, J.R. & Eriksson, T. Plant regeneration from protoplasts of Panax ginseng (C.A. Meyer) through somatic embryogenesis. Plant Cell Reports 10, 277–281 (1991). https://doi.org/10.1007/BF00193141

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  • DOI: https://doi.org/10.1007/BF00193141

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