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Therapeutic intervention with chicken egg white ovomacroglobulin and a new quinolone on experimental Pseudomonas keratitis

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Abstract

⊎ Background: Chicken egg white ovomacroglobulin (ovoM) is a potent protease inhibitor with broad-spectrum activity against various proteases. The combined effects of ovoM and the new quinolone, ofloxacin (OFLX) on experimental Pseudomonas aeruginosa keratitis were investigated. ⊎ Methods: The in vitro inhibitory effects of ovoM on protease activity in culture fluid of clinically isolated P. aeruginosa and on activity of human neutrophil elastase and cathepsin G were assayed using azo-casein as substrate. Albino rabbits received intrastromal injection of the isolated Pseudomonas strain (1 × 105 colony-forming units). At 16 h after inoculation, three treatment groups -0.1% ovoM alone, 0.3% OFLX alone, and a combination of both —and a non-treatment control group were tested. ⊎ Results: Protease activity in the culture solution and human neutrophil elastase was inhibited by ovoM, whereas cathepsin G was not inhibited effectively. In vivo additive therapeutic effects of ovoM and OFLX were observed at 96 h (P < 0.05 compared with OFLX alone). ⊎ Conclusion: The results indicate that inhibition of proteolytic activity with ovoM is useful in preventing stromal degradation in P. aeruginosa keratitis.

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Authors and Affiliations

  1. Department of Ophthalmology, Kumamoto University School of Medicine, Kumamoto 860, Japan

    Shin-ichi Miyagawa, Ryuji Kamata, Koki Matsumoto & Ryo-ichi Okamura

  2. Department of Microbiology, Kumamoto University School of Medicine, Kumamoto 860, Japan

    Shin-ichi Miyagawa & Hiroshi Maeda

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  1. Shin-ichi Miyagawa
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  2. Ryuji Kamata
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  3. Koki Matsumoto
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  4. Ryo-ichi Okamura
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  5. Hiroshi Maeda
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Miyagawa, Si., Kamata, R., Matsumoto, K. et al. Therapeutic intervention with chicken egg white ovomacroglobulin and a new quinolone on experimental Pseudomonas keratitis. Graefe's Arch Clin Exp Ophthalmol 232, 488–493 (1994). https://doi.org/10.1007/BF00195359

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  • DOI: https://doi.org/10.1007/BF00195359

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