Skip to main content
SpringerLink
Log in

Tumour inhibitory effects of TCGF/IL-2/-containing preparations

  • Original Articles
  • Published:
Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Summary

Supernatants from ConA-stimulated rat spleen cell cultures and from cultures of PMA-stimulated murine lymphoma subline EL-4TF were found to contain TCGF and to inhibit growth of a transplantable, MC-induced sarcoma MC11 in syngeneic mice. Tumour-inhibitory effects of the supernatants were dependent on local and repeated administration. Prior to use of the supernatants obtained from PMA-stimulated EL-4TF cell cultures, the dialysable PMA had to be removed; contamination with PMA was found to abolish the tumour-inhibitory effect of the supernatants and to produce enhancement of tumour growth. A significant tumour-inhibitory effect has also been obtained with partially purified TCGF prepared from culture supernatants of cloned EL-4TF cells by ammonium sulphate precipitation, ion-exchange (FPLC) chromatography, and AcA 44 Ultrogel filtration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

TCGF:

T cell growth factor

IL-2:

interleukin 2

Con A:

concanavalin A

3H-TDR:

tritiated thymidine

PMA:

4-β-phorbol-12-β-myristate-13-α-acetate

NK:

natural killer cells

B10:

C57BL10/ScSnPH mice

FPLC:

fast protein liquid chromatography

BSA:

bovine serum albumin

OVA:

ovalbumin

SBI:

trypsin inhibitor from soybean

Cyt C:

cytochrome C

OD:

optical density

PBS:

phosphate-buffered saline

MC:

3-methylcholanthrene

References

  1. Bubeník J, Indrová M, Němečková Š, Malkovský M, von Broen B, Pálek V, Anderlíková J (1978) Solubilized tumour-associated antigens of methylcholanthrene-induced mouse sarcomas. Comparative studies by in vitro sensitization of lymph-node cells, macrophage electrophoretic mobility assay and transplantation tests. Int J Cancer 21:348–355

    Google Scholar 

  2. Bubeník J, Perlmann P, Indrová M, Šímová J, Jandlová T, Neuwirt J (1983) Growth inhibition of an MC-induced mouse sarcoma by TCGF (IL-2)-containing preparations. Cancer Immunol Immunother 14:205–206

    Google Scholar 

  3. Bubeník J, Indrová M, Šímová J, Kypěnová H, Bubeníková D, Doležalová K (1984) Phorbol myristate acetate-stimulated production of interleukin 2 by T-cell lymphoma and constitutive production by derived hybridomas. Neoplasma 31:497–505

    Google Scholar 

  4. Caplan B, Gibbs C, Paetkau V (1981) Properties of sodium dodecyl sulphate-denatured interleukin 2. J Immunol 126:1351–1354

    Google Scholar 

  5. Cheever MA, Greenberg PD, Fefer A, Gillis S (1982) Augmentation of the anti-tumor therapeutic efficacy of long-term cultured T lymphocytes by in vivo administration of purified interleukin 2. J Exp Med 155:968–980

    Google Scholar 

  6. Conlon PJ, Hefeneider SH, Henney CS, Gillis S (1982) The effects of interleukin 2 on primary in vivo immune responses. In: Fefer A, Goldstein A (eds) The potential role of T cells in cancer therapy. Raven, New York, pp 113–125

    Google Scholar 

  7. Donohue JH, Rosenberg SA (1983) The fate of interleukin-2 after in vivo administration. J Immunol 130:2203–2208

    Google Scholar 

  8. Farrar JJ, Fuller-Farrar J (1981) Progress in the purification of murine thymoma-derived interleukin 2. In: Goldstein AL, Chirigos MA (eds) Lymphokines and thymic hormones: their potential utilization in cancer therapeutics. Raven, New York, pp 49–55

    Google Scholar 

  9. Gillis S, Ferm MM, Ou W, Smith KA (1978) T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol 120:2027–2032

    Google Scholar 

  10. Gillis S, Smith KA, Watson J (1980) Biochemical characterization of lymphocyte regulatory molecules. II. Purification of a class of rat and human lymphokines. J Immunol 124:1954–1962

    Google Scholar 

  11. Gorer PA, Amos DB (1956) Passive immunity of mice against C57BL leukosis EL-4 by means of iso-immune serum. Cancer Res 16:338–343

    Google Scholar 

  12. Granelli-Piperno A, Vassalli J-D, Reich E (1981) Purification of murine T cell growth factor. A lymphocyte mitogen with helper activity. J Exp Med 154:422–431

    Google Scholar 

  13. Grönvik K-O, Andersson J, Alm G, Stern P (1983) T-cell growth factor contains activity that supports natural killer activity in vitro. Scand J Immunol 18:307–317

    Google Scholar 

  14. Grosz CG, Roopernian DC, Bach FH (1983) Phorbol myristate acetate and in vitro T lymphocyte function. I. PMA may contaminate lymphokine preparations and can interfere with interleukin bioassays. J Immunol 130:1764–1769

    Google Scholar 

  15. Hefeneider SH, Conlon PJ, Henney CS, Gillis S (1983) In vivo interleukin 2 administration augments the generation of alloreactive cytolytic T lymphocytes and resident natural killer cells. J Immunol 130:222–227

    Google Scholar 

  16. Henney CS, Kuribayashi K, Kern DE, Gillis S (1981) Interleukin 2 augments natural killer cell activity. Nature 291:335–338

    Google Scholar 

  17. Kramlová M, Přistoupil T, Fričová V, Kraml J, Berglund R, Kantardjiev N (1982) Fast protein liquid chromatography of pyridoxalated and glutaraldehyde-treated human haemoglobin. J Chromatogr 249:403–404

    Google Scholar 

  18. Mochizuki D, Watson J, Gillis S (1980) Biochemical and biologic characterization of lymphocyte regulatory molecules. IV. Purification of interleukin 2 from a murine T cell lymphoma. J Immunol 125:2579–2583

    Google Scholar 

  19. Mühlradt PF, Opitz HG (1982) Clearance of interleukin 2 from the blood of normal and T-cell-depleted mice. Eur J Immunol 12:983–985

    Google Scholar 

  20. Paetkau V, Mills GB, Bleackley RC (1982) Enhancement of anti-tumor immune responses with interleukin 2. In: Fefer A, Goldstein A (eds) The potential role of T cells in cancer therapy. Raven, New York, pp 147–159

    Google Scholar 

  21. Přistoupil T, Kramlová M, Fričová V, Kraml J (1983) The reproducibility of fast protein liquid chromatography of pyridoxalated haemoglobin copolymerized with serum albumin. J Chromatogr 280:165–166

    Google Scholar 

  22. Smith KA (1980a) T-cell growth factor. Immunol Rev 51:337–357

    Google Scholar 

  23. Smith KA (1980b) Continuous cytotoxic T-cell lines. In: Contemporary topics in immunobiology. Plenum, New York, pp 139–155

    Google Scholar 

  24. Smith KA, Ruscetti FW (1981) T cell growth factor and the culture of cloned functional T cells. Adv Immunol 31:137–175

    Google Scholar 

  25. Wagner H, Hardt C, Heeg K, Röllinghoff M, Pfizenmaier K (1980) T-cell-derived helper factor allows in vivo induction of cytotoxic T cells in nu/nu mice. Nature 284:278–280

    Google Scholar 

  26. Watson J, Gillis S, Marbrook J, Mochizuki D, Smith KA (1979) Biochemical and biological characterization of lymphocyte regulatory molecules. I. Purification of a class of murine lymphokines. J Exp Med 150:849–861

    Google Scholar 

  27. Welte K, Wang CY, Mertelsmann R, Venuta S, Feldman SP, Moore MA (1982) Purification of human interleukin 2 to apparent homogeneity and its molecular heterogeneity. J Exp Med 156:454–464

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Immunology, The Wenner-Gren Institute, S-10691, Stockholm, Sweden

    P. Perlmann & K. Berzins

  2. First Institute of Medical Chemistry, Faculty of General Medicine, Charles University, 120 00, Prague, Czechoslovakia

    J. Kraml

  3. Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Flemingovo nám. 2, 166 37, Prague, Czechoslovakia

    J. Bubeník, M. Indrová, P. Perlmann, K. Berzins, O. Mach, J. Kraml & A. Toulcová

Authors
  1. J. Bubeník
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Indrová
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Perlmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Berzins
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. O. Mach
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. Kraml
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. Toulcová
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bubeník, J., Indrová, M., Perlmann, P. et al. Tumour inhibitory effects of TCGF/IL-2/-containing preparations. Cancer Immunol Immunother 19, 57–61 (1985). https://doi.org/10.1007/BF00199313

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00199313

Keywords

Search

Navigation