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Rapid purification of two lipase isoenzymes from Candida rugosa

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Biotechnology Techniques

Summary

We describe a two-step method for the purification of two lipases (lipases A and B) from C. rugosa. The purification procedure includes Phenyl-Sepharose and Sephacryl HR 100 chromatographies. The enzymes obtained were pure according to criteria of specific activity and neutral sugar content.

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Author notes

  1. M. Luisa Rúa

    Present address: Departamento de Biología Fundamental. Facultad de Ciencias, Universidad de Vigo, As Lagoas S/N, 32004, Ourense, Spain

Authors and Affiliations

  1. Unidad de Biocatálisis, Instituto de Catálisis, CSIC, 28049, Madrid, Spain

    M. Luisa Rúa & Antonio Ballesteros

Authors
  1. M. Luisa Rúa
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  2. Antonio Ballesteros
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Rúa, M.L., Ballesteros, A. Rapid purification of two lipase isoenzymes from Candida rugosa . Biotechnol Tech 8, 21–26 (1994). https://doi.org/10.1007/BF00207628

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  • DOI: https://doi.org/10.1007/BF00207628

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