Abstract
Photobleaching of disodium fluorescein dissolved in water is experimentally investigated using laser induced fluorescence (LIF). It is demonstrated that significant photobleaching occurs on the millisecond time scale, resulting in a large decrease in the fluorescence signal emanating from a constant concentration sample. The importance of avoiding photobleaching when using LIF with disodium fluorescein for concentration measurements in water flow experiments is demonstrated. A half-life for photobleaching is introduced and measured for disodium fluorescein and is shown to be a more appropriate measure than the traditional ‘bleaching quantum efficiency’. It is demonstrated that the photobleaching of disodium fluorescein is at least partially reversible.
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Useful discussions and comments were provided by Professor K. R. Sreenivasan, Dr. Ray Glynn Holt and Dr. Michael A. Tanoff. This work was supported by the Air Force Office of Scientific Research.
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Saylor, J.R. Photobleaching of disodium fluorescein in water. Experiments in Fluids 18, 445–447 (1995). https://doi.org/10.1007/BF00208467
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DOI: https://doi.org/10.1007/BF00208467