Summary
The objectives of this study were: (i) to develop a tissue culture technique for the evaluation of Fe efficiency in soybean, and (ii) to compare the laboratory technique with field Fe chlorosis scores. Nineteen genotypes that had low and high levels of Fe efficiency were evaluated in the laboratory and at five field locations. Friable callus was induced from epicotyl sections, weighed, and placed on two different modified Murashige and Skoog media; one low in α-naphthaleneacetic acid and the other low in Fe. Callus growth was rated as lack of growth compared to respective controls. As an example, Fe-inefficient cultivars (‘Asgrow A3205’ and ‘Pride B216’) had significantly reduced growth compared to Fe-efficient germ plasm lines (‘All’ and ‘A14’). Correlation between the laboratory and field chlorosis rating was highest for the low auxin medium (r 2 = 0.78), although correlation for the low Fe medium was also significant (r 2 = 0.72). These results show that in vitro evaluation for Fe efficiency can be a useful tool for plant breeders.
Similar content being viewed by others
References
Barwale UB, Kerns HR, Widholm JM (1986) Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta 67:473–481
Bernard RL (1974) Notice of release of Clark and Harosoy isolines. Soybean Genet Newsl 1:66–75
Bernard RL, Cremeens CR (1988) Registration of ‘Corsoy 79’ soybean. Crop Sci 28:1027
Chaney RL, Brown JC, Tiffin LO (1972) Obligatory reduction of ferric chelates in iron-uptake by soybeans. Plant Physiol 50:208–213
Cianzio SR de, Fehr WR, Anderson IC (1979) Genotypic evaluation for iron deficiency chlorosis in soybeans by visual scores and chlorophyll concentration. Crop Sci 19:644–646
Cianzio SR de, Fehr WR (1982) Variation in the inheritance of resistance to iron deficiency chlorosis in soybeans. Crop Sci 22:433–434
Coulombe B A, Chaney RL, Wiebold WJ (1984) Use of bicarbonate in screening soybeans for resistance to iron chlorosis. J Plant Nutr 7:411–425
Fehr WR, Bahrenfus JB (1980) Registration of a germ plasm line of soybean. Crop Sci 20:419
Fehr WR, Trimble MW (1982) Minimizing soybean yield loss from iron deficiency chlorosis. Iowa Coop Ext Pamphlet Pm-1059
Fehr WR, Caviness CE, Burmood DT, Pennington JS (1971) Stage of development descriptions for soybeans, Glycine max (L.) Merrill. Crop Sci 11:929–931
Fehr WR, Voss BK, Cianzio SR de (1984) Registration of a germ plasm line of soybean, A7. Crop Sci 24:390–391
Frank SJ, Fehr WR (1983) Band application of sulfuric acid or elemental sulfur for control of Fe-deficiency chlorosis of soybeans. Agron J 75:451–454
Jessen HJ, Fehr WR, Piper TE (1986) Alternatives to conventional field evaluation for genetic selection for Fe-efficiency of soybean. J Plant Nutr 9:345–353
Jessen HJ, Fehr WR, Cianzio SR de (1988) Registration of germ plasm lines of soybean, A11, A12, A13, A14, A15. Crop Sci 28:204
Johnson HW (1958) Registration of soybean varieties, VI. Agron J 50:690–691
Karkosh AE, Walker AK, Simmons JJ (1988) Seed treatment for control of iron-deficiency chlorosis of soybean. Crop Sci 28:369–370
Landsberg EC (1984) Regulation of iron-stress-response by whole-plant activity. J Plant Nutr 7:609–621
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497
Olsen RA, Brown JC (1980) Factors related to iron uptake by dicotyledonous and monocotyledonous plants. I. pH and reductant. J Plant Nutr 2:629–645
Randall GW (1977) Iron chlorosis in soybeans. Dep Soil Sci Soils Ser 95:124–127. University of Minnesota, St. Paul/MN
Sain SL, Johnson GV (1984) Selection of iron-efficient soybean cell lines using plant cell suspension culture. J Plant Nutr 7:389–398
Tachibana H, Bahrenfus JB, Fehr WR (1983) Registration of BSR 201 soybean. Crop Sci 23:186
Walker AK, Fioritto RJ, St Martin SK, Schmitthenner AF, Cooper RL, Martin RJ (1986) Registration of ‘Zane’ soybean. Crop Sci 26:199
Weiss MG (1943) Inheritance and physiology of efficiency in iron utilization in soybeans. Genetics 28:253–268
Weiss MG, Stevenson TM (1955) Registration of soybean varieties, V. Agron J 47:541–543
Wilcox JR (1988) The uniform soybean tests northern states. West Lafayette Report is copied and bound by USDA-ARS Publ. is USDA-ARS without a publication number
Wilcox JR, Athow KL, Abney TS, Laviolette FA, Richards TL (1980) Registration of Century soybean. Crop Sci 20:415
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Stephens, P.A., Widholm, J.M. & Nickell, C.D. Iron-deficiency chlorosis evaluation of soybean with tissue culture. Theoret. Appl. Genetics 80, 417–420 (1990). https://doi.org/10.1007/BF00210082
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00210082