Abstract
We have used a fluorescent in situ hybridization procedure to detect human satellite 1 DNA, the simple sequence family that constitutes the non-male-specific fraction of classical satellite 1 DNA. Satellite 1 appears to be located on pericentromeric regions of chromosomes 3, 4 and 13, and on satellites of each acrocentric chromosome. These results suggest a possible relationship between quinacrine fluorescence of heterochromatin and DNA composition. Furthermore, by means of multicolour in situ hybridization, we have spatially resolved satellite 1 sequences and centromeric α-satellite within heterochromatic blocks.
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Tagarro, I., Wiegant, J., Raap, A.K. et al. Assignment of human satellite 1 DNA as revealed by fluorescent in situ hybridization with oligonucleotides. Hum Genet 93, 125–128 (1994). https://doi.org/10.1007/BF00210595
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DOI: https://doi.org/10.1007/BF00210595