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Ultracytochemical localization of Ca++-ATPase activity in the paraphyseal epithelial cells of the frog, Rana esculenta

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Summary

Ca++-ATPase activity was studied ultracytochemically (cf. Ando et al. 1981) in the paraphysis cerebri of the frog. An intense reaction was demonstrated on the plasmalemma of the microvilli at the apical pole of paraphyseal cells; in contrast, the basolateral plasmalemma showed only a slight staining. In addition, mitochondria, gap junctions, cilia, and cytoplasmic elements (e.g., microfilaments) displayed Ca++-ATPase activity. Variation of the Ca++-concentration in the incubation medium from 0.1 mM to 100 mM altered the Ca++-ATPase activity of the cell organelles. The substitution of Ca-by Mg-ions resulted in a conspicuous decrease in the enzyme activity, especially on the apical plasmalemma. Ca++-ATPase activity is claimed to be involved in a number of extra-and intracellular functions. In comparison to the epithelium of the adjacent choroid plexus the paraphyseal epithelial cell is thought to be a principal Ca-ion regulator of the cerebrospinal fluid in frogs.

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Authors and Affiliations

  1. Department of Ophthalmology, Faculty of Medicine, Kyoto University, Kyoto, Japan

    Satoki Ueno

  2. Department of Anatomy and Cytobiology, Justus Liebig University, Giessen, Federal Republic of Germany

    Hikmet Umar, Heinz Joachim Bambauer & Manfred Ueck

Authors
  1. Satoki Ueno
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  2. Hikmet Umar
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  3. Heinz Joachim Bambauer
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  4. Manfred Ueck
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Fellow of the Alexander von Humboldt Foundation

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Ueno, S., Umar, H., Bambauer, H.J. et al. Ultracytochemical localization of Ca++-ATPase activity in the paraphyseal epithelial cells of the frog, Rana esculenta . Cell Tissue Res. 235, 3–11 (1984). https://doi.org/10.1007/BF00213716

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