Summary
Antibodies raised against glial fibrillary acidic protein (GFA), S-100 protein (S100) and glutamine synthetase (GS) are currently used as glial markers. The distribution of GFA, S100 and GS in the ependyma of the rat subcommissural organ (SCO), as well as in the adjacent nonspecialized ventricular ependyma and neuropil of the periaqueductal grey matter, was studied by use of the immunocytochemical peroxidase-antiperoxidase technique. In the neuropil, GFA, S100 and GS were found in glial elements, i.e., in fibrous (GFA, S100) and protoplasmic astrocytes (S100, GS). The presence of S100 in the majority of the ventricular ependymal cells and tanycytes, and the presence of GFA in a limited number of ventricular ependymal cells and tanycytes confirm the glial nature of these cells. The absence of S100, GFA and GS from the ependymocytes of the SCO, which are considered to be modified ependymal cells, suggests either a non-astrocytic lineage of these cells or an extreme specialization of the SCO-cells as glycoprotein-synthesizing and secreting elements, a process that may have led to the disappearance of the glial markers.
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References
Anderson MJ, Swanson KA, Waxman SG, Eng LF (1984) Glial fibrillary acidic protein in regenerating teleost spinal cord. J Histochem Cytochem 10:1099–1106
Bignami A, Dahl D (1980) Astrocyte-specific protein and neuroglial differentiation. An immunofluorescence study with antibodies to the glial fibrillary acidic protein. J Comp Neurol 153:27–38
Bock R (1966) Über die Darstellbarkeit neurosekretorischer Substanz mit Chromalaun-Gallocyanin im supraoptico-hypophysären System beim Hund. Histochemie 6:362–369
Bouchaud C, Arluison M (1977) Serotoninergic innervation of ependymal cells in the rat subcommissural organ. A fluorescence electron microscopic and radioautographic study. Biol Cell 30:61–64
Bullon MM, Gago TA, Ruiz BF, Aguirre C (1984a) Glial fibrillary acidic (GFA) protein in rat spinal cord. An immunoperoxidase study in semithin sections. Brain Res 309:79–83
Bullon MM, Gago TA, Ruiz BF, Aguirre C (1984b) Glial fibrillary acidic protein (GFAP) in spinal cord of postnatal rat. An immunoperoxidase study in semithin sections. Develop Brain Res 14:129–133
Caldani M, Rolland B, Fages C, Tardy M (1982) Glutamine synthetase activity during mouse brain development. Experentia 38:1199–1202
Cicero TJ, Ferrendelli JA, Suntzeff V, Moore BW (1977) Regional changes in CNS levels of the S-100 and 14–3–2 proteins during development and aging of the mouse. J Neurochem 19:2119–2125
De Vitry F, Picart R, Jacquet C, Legault L, Dupouey P, Tixier Vidal A (1980) Presumptive common precursor for neuronal and glial cell lineages in mouse hypothalamus. Proc Natl Acad Sci USA 77:4165–4169
De Vitry F, Picart R, Jacque C, Tixier-Vidal A (1981) Glial fibrillary acidic protein. Dev Neurosci 4:457–460
Diederen JHB, Vullings HGB (1980) Comparison of several parameters related to the secretory activity of the subcommissural organ in European green frogs. Cell Tissue Res 212:383–394
Dixon RG, Eng LF (1981) Glial fibrillary acidic protein in the retina of the developing albino rat: an immunoperoxidase study of paraffin-embedded tissue. J Comp Neurol 195:305–321
Gamrani H, Belin MF, Aguera M, Calas A, Pujol JF (1981) Radioautographic evidence for an innervation of the subcommissural organ by GABA containing nerve fibers. J Neurocytol 10:411–424
Haan EA, Boss BD, Cowan WM (1982) Production and characterization of monoclonal antibodies against the “brain-specific” proteins 14–3–2 and S-100. Proc Natl Acad Sci 79:7585–7589
Hallermayer K, Hamprecht B (1984) Cellular heterogeneity in primary cultures of brain cells revealed by immunocytochemical localization of glutamine synthetase. Brain Res 295:1–11
Hallermayer K, Harmening C, Hamprecht B (1981) Cellular localization and regulation of glutamine synthetase in primary cultures of brain cells from newborn mice. J Neurochem 31:33–52
Hess J, Sterba G (1973) Studies concerning the function of the complex subcommissural organ liquor fibre. The binding ability of the liquor fibre to pyrocatechin derivatives and its functional aspects. Brain Res 58:303–312
Jessen KR, Mirsky R (1983) Astrocyte-like glia in the peripheral nervous system: an immunohistochemical study of enteric glia. J Neuroscience 11:2206–2218
Kimura S, Kato K, Semba R, Isobe T (1984) Regional distribution of S-100ao (αα), S-100a (αβ) and S-100b (ββ) in the bovine central nervous tissue determined with a sensitive enzyme immunoassay system. Neuroscience 4:513–518
Lauriola L, Michetti F, Sentinelli S, Cocchia D (1984) Detection of S100 labelled cell in nasopharyngeal carcinoma. J Clin Pathol 37:1235–1238
Léger L, Degueurce A, Lundberg JJ, Pujol JF, Møllgärd K (1983) Origin and influence of the serotoninergic innervation of the subcommissural organ of the rat. Neuroscience 10:411–423
Leonhardt H (1980) Ependym and circumventriculäre Organe. In: Oksche A, Vollrath L (eds) Neuroglia I. Handbuch der mikroskopischen Anatomie des Menschen. Band IV 10. Teil. Springer, Berlin Heidelberg New York 4:177–665
Levitt P, Cooper ML, Rakic P (1981) Coexistence of neuronal and glial precursor cells in the cerebral ventricular zone of the fetal monkey: an ultrastructural immunoperoxidase analysis. J Neurosci 1:27–39
Lösecke W, Naumann W, Sterba G (1984) Preparation and discharge of secretion in the subcommissural organ of the rat. An electron-microscopic immunocytochemical study. Cell Tissue Res 235:201–206
Ludwin SK, Kosek JC, Eng LF (1975) The topographical distribution of S-100 and GFA proteins of the adult rat brain: an immunohistochemical study using horseradish peroxidase-labelled antibodies. J Comp Neurol 165:197–208
Mestres P (1978) Old and new concepts about circumventricular organs: an overview. Scan Electron Microsc 11:137–142
Møller M, Ingild A, Bock E (1978) Immunohistochemical demonstration of S-100 protein and GFA protein in interstitial cells of rat pineal gland. Brain Res 140:1–13
Møllgård K, Wiklund L (1979) Serotoninergic synapses on ependymal and hypendymal cells of the rat subcommissural organ. J Neurocytol 8:445–467
Møllgård K, Lundberg JJ, Wiklund L, Lachenmayer L, Baumgarten HG (1978) Morphological consequences of serotonin neurotoxin administration: neuron-target cell interaction in the subcommissural organ. Ann NY Acad Sci 305:262–268
Nakazato Y, Ishizeki J, Takahashi K, Yamaguchi H, Kamei T, Takesada M (1982) Localization of S-100 protein and glial fibrillary acidic protein-related antigen in pleomorphic adenoma of the salivary glands. Lab Invest 46:621–626
Norenberg MD (1979) Distribution of glutamine synthetase in the rat central nervous system. J Histochem Cytochem 27:756–762
Norenberg MD, Hernandez AM (1979) Fine structural localization of glutamine synthetase in astrocytes of rat brain. Brain Res 161:303–310
Onteniente B, Kimura H, Maeda T (1983) Comparative study of the glial fibrillary acidic protein in vertebrates by PAP immunohistochemistry. J Comp Neurol 215:427–436
Patel AJ, Hunt A, Tahourdin CSM (1983a) Regional development of glutamine synthetase activity in the rat brain and its association with the differentiation of astrocytes. Develop Brain Res 8:31–37
Patel AJ, Hunt A, Tahourdin CSM (1983b) Regulation of in vivo glutamine synthetase activity by glucocorticoids in the developing rat brain. Develop Brain Res 10:83–91
Patel AJ, Weir MD, Hunt A, Tahourdin CSM, Thomas DGT (1985) Distribution of glutamine synthetase and glial fibrillary acidic protein and correlation of glutamine synthetase with glutamate decarboxylase in different regions of the rat central nervous system. Brain Res 331:1–9
Peters A, Palay SL, Webster H (1976) The fine structure of the nervous system: The neurons and supporting cells. Saunders Company, Philadelphia London Toronto, p 264–279
Rodriguez EM, Oksche A, Hein S, Rodriguez S, Yulis R (1984) Spatial and structural interrelationships, between secretory cells of the subcommissural organ and blood vessels. An immunocytochemical study. Cell Tissue Res 237:443–449
Roessmann U, Velasco ME, Sindely SD, Gambetti P (1980) Glial fibrillary acidic protein (GFAP) in ependymal cells during development. An immunocytochemical study. Brain Res 200:13–21
Schmechel DE, Marangos PJ, Zig AP, Brightman MW, Goodwin FK (1978) Brain enolases as specific marker of neuronal and glial cells. Science 199:313–315
Sterba G, Klein I, Naumann W, Petter H (1981) Immunocytochemical investigation of the subcommissural organ in the rat. Cell Tissue Res 218:659–662
Sternberger LA, Hardy PH, Cuculis JJ, Mayer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Tardy M, Rolland B, Fages C, Caldani M (1984) Astroglia cells: glucocorticoid target cells in the brain. Clin Neuropharmacol 7–4:296–302
Valentino KL, Jones EG, Kane SA (1983) Expression of GFAP immunoreactivity during development of long fiber tracts in the rat CNS. Develop Brain Res 9:317–336
Weissmann-Nanopoulos D, Belin MF, Didier M, Aguera M, Partisani M, Maitre M, Pujol JF (1983) Immunohistochemical evidence for neuronal and non-neuronal synthesis of GABA in the rat subcommissural organ. Neurochem Intern 5:785–791
Yen SH, Fields KL (1981) Antibodies to neurofilament, glial filament, and fibroblast intermediate filament proteins bind to different cell types of the nervous system. J Cell Biol 88:115–126
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Didier, M., Harandi, M., Aguera, M. et al. Differential immunocytochemical staining for glial fibrillary acidic (GFA) protein, S-100 protein and glutamine synthetase in the rat subcommissural organ, nonspecialized ventricular ependyma and adjacent neuropil. Cell Tissue Res. 245, 343–351 (1986). https://doi.org/10.1007/BF00213941
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DOI: https://doi.org/10.1007/BF00213941