Summary
The formation and secretion of ecdysteroid by the prothoracic gland cells of Galleria mellonella (Insecta, Lepidoptera) were investigated electron microscopically and immunocytochemically. The moulting hormone ecdysone becomes first evident in vesicles and tubules of the smooth endoplasmic reticulum (SER). The SER forms secretory granules in which ecdysone was shown immunocytochemically. The Golgi apparatus seems not to be directly involved in ecdysone secretion. The secretory granules are released from the cells by exocytosis.
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Beaulaton JA (1968) Modifications ultrastructurales des cellules sécrétrices de la glande prothoracique de vers à soie au cours des deux derniers âges larvaires. I. Le chondriome, et ses relations avec le reticulum agranulaire. J Cell Biol 39:501–525
Birkenbeil H, Eckert M (1982) Untersuchungen zur Methodik des ultrastrukturellen immuncytochemischen Nachweises von Ecdysteroiden. Acta Histochem 71:133–143
Birkenbeil H, Eckert M, Gersch M (1979) Electron microscopical-immunocytochemical evidence of ecdysteroids in the prothoracic gland of Galleria mellonella. Cell Tissue Res 200:285–290
Bollenbacher WE, Zvenko H, Kumaran AK, Gilbert LI (1978) Changes in ecdysone content during postembryonic development of the waxmoth, Galleria mellonella: the role of the ovary. Gen Comp Endocrinol 34:169–179
Eibisch H, Gersch M, Eckert M, Böhm G-A (1980) Investigations of the ecdysteroid production in the prothoracic glands and of the ecdysteroid level in the hemolymph of Periplaneta americana larvae by means of radioimmunoassay (RIA) with an anti-ecdysterone serum. Zool. Jb Physiol 84:153–163
Gemmel RT, Stacy BD (1979) Granule secretion by the luteal cells of the sheep: the fate of the granule membrane. Cell Tissue Res 197:413–419
Gersch M, Birkenbeil H, Ude J (1975) Ultrastructure of the last instar of Galleria mellonella in relation to the state of development. Cell Tissue Res 160:389–397
Geyer G (1969) Ultrahistochemie. VEB Gustav Fischer Verlag, Jena, p 227
Gras R, Beaulaton J (1979) Développement cyclique du reticulum endoplasmique agranulaire dans les glandes thoraciques (glandes ecdysiales) de Rhodnius prolixus Stäl (Insecte: Hétéroptère) aux deux derniers stades larvaires. Experientia 35:386–388
Gronemeyer H, Pongs O (1980) Localization of ecdysterone on polytene chromosomes of Drosophila melanogaster. Proc Natl Acad Sci USA 77:2108–2112
Hwang-Hsu K, Reddy G, Krishna Kumaran A, Bollenbacher WE, Gilbert LI (1979) Correlations between juvenile hormone esterase activity, ecdysone titre and cellular reprogramming in Galleria mellonella. J Insect Physiol 25:105–111
Locke M (1969) The ultrastructure of the oenocytes in the molt/intermolt cycle of an insect. Tissue Cell 1:103–154
Maróy P, Tarnóy K (1978) Moulting hormone level during the last instar of Galleria mellonella larva. J Insect Physiol 24:325–327
Nussdorfer GG (1980) Cytophysiology of the adrenal zona glomerulosa. Int Rev Cytol 64:307–368
Nussdorfer GG, Mazzocchi G (1982) Stereological and biochemical investigations on a possible aldosterone-containing secretory organelle in the zona glomerulosa of rat adrenal gland. Anat Anz 151:74–81
Nussdorfer GG, Mazzocchi G, Meneghelli V (1978) Cytophysiology of the adrenal zona fasciculata. Int Rev Cytol 55:291–365
Pearce RB, Cronshaw J, Holmes WN (1977) The fine structure of the interrenal cells of the duck (Anas platyrhynchos) with evidence for the possible exocytotic release of steroids. Cell Tissue Res 183:203–220
Piepho H (1938) Wachstum und totale Metamorphose an Hautimplantaten bei der Wachsmotte Galleria mellonella. Biol Zbl 58:356–366
Rees HH, Davies TG, Dinan LN, Lockley WJ, Goodwin TW (1980) The biosynthesis of ecdysteroids. In: Hoffmann JA (ed) Progress in ecdysone research. North Holland/Elsevier, Amsterdam
Romer F (1971) Die Prothorakaldrüsen der Larve von Tenebrio molitor L (Tenebrionidae, Coleoptera) und ihre Veränderungen während eines Häutungszyklus. Z. Zellforsch 122:425–455
Romer F (1973) Feinstrukturelle Merkmale der Oenocyten pterygoter Insekten. Verh d Dtsch Zool Ges 66:65–70
Sehnal F (1966) Kritisches Studium der Bionomie und Biometrik der in verschiedenen Lebensbedingungen gezüchteten Wachsmotte, Galleria mellonella (Lepidoptera). Z wiss Zool 174:53–82
Tamaoki B (1973) Steroidogenesis and cell structure. J Steroid Biochem 4:89–118
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Supported by the Sächsische Akademie der Wissenschaften zu Leipzig
The author is grateful to Mrs. Angelika Schmidt for her excellent assistance
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Birkenbeil, H. Ultrastructural and immunocytochemical investigation of ecdysteroid secretion by the prothoracic gland of the waxmoth Galleria mellonella . Cell Tissue Res. 229, 433–441 (1983). https://doi.org/10.1007/BF00214984
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DOI: https://doi.org/10.1007/BF00214984