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Ultracytochemistry of the synaptic ribbons in the rat pineal organ

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Summary

The synaptic complexes of the rat pinealocytes are neither cholinergic nor adrenergic. In the synaptic vesicles, a neurotransmitter carrier substance of lipid nature reacting with OsO4-Zn I2 mixture (similar to that present in both cholinergic and adrenergic vesicles) was not found.

In addition, there were no indications of glucose-6-phosphatase or thiamine-pyrophosphatase activity in the synaptic vesicles. Thus, it appears that the synaptic vesicles do not originate from the rough or smooth endoplasmic reticulum.

The synaptic ribbons do not contain carbohydrates, are of protein nature and possess some chemical resemblance to microtubules and microtubular bouquets.

Appropriate ultracytochemical reactions have not shown detectable quantities of sodium and calcium ions in pinealocyte synaptic complexes.

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Authors and Affiliations

  1. Institute of Histology and Embryology, University of Lausanne, Switzerland

    Radivoj Krstić

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  1. Radivoj Krstić
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Dedicated to Professor Dr. med. G. Töndury on the occasion of his 70th birthday.

Grateful acknowledgment is made to Mr. P.-A. Milliquet for technical assistance and to Dr. T. Jalanti (C.M.E., Lausanne) for his help in the use of the X-ray microanalyser.

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Krstić, R. Ultracytochemistry of the synaptic ribbons in the rat pineal organ. Cell Tissue Res. 166, 135–143 (1976). https://doi.org/10.1007/BF00215133

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  • DOI: https://doi.org/10.1007/BF00215133

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