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Hydration-state-responsive proteins link cold and drought stress in spinach

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Abstract

Spinach (Spinacia oleracea L.) seedlings exposed to low nonfreezing temperatures (0–10° C) that promote cold acclimation, synthesize a variety cold-acclimation proteins and at the same time acquire a greater ability to withstand cellular dehydration imposed by the freezing of tissue water. Two of these proteins (160 and 85 kDa) become more abundant over time at low temperature. In addition, a small decline in tissue water status from a maximally hydrated state also appears to be associated with an initiation of the accumulation of these proteins at a noninductive temperature. Imposing a severe water stress on young seedlings grown at 25° C by withholding water leads to substantial accumulation of the 160- and 85-kDa proteins, and maximal induction of freezing tolerance. This evidence implies that responses to cold acclimation and water stress involve common mechanisms, and further establishes the linkage of these two proteins with stresses having an osmotic component.

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Abbreviations

ABA:

abscisic acid

CAP:

cold-acclimation protein

kDa:

kilodaltons

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Authors and Affiliations

  1. Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, 32611-0512, Gainesville, FL, USA

    Charles Guy, Dale Haskell, Lisa Neven & Chris Smelser

  2. Department of Pathology and Laboratory Medicine, University of Florida, 32610, Gainesville, FL, USA

    Paul Klein

Authors
  1. Charles Guy
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  2. Dale Haskell
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  3. Lisa Neven
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  4. Paul Klein
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  5. Chris Smelser
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Additional information

We thank T. Sinclair and K. Cline for critical reading and discussions, N. Denslow for assistance with protein sequencing methods, and L. Greene, S. Henry for preparing the monoclonal antibodies. The work was made possible by support from the USDA Competitive Grants Program No. 90-37280-5527, the Institute for Food and Agricultural Sciences, and through access to the protein sequencing and hybridoma facilities of the Interdisciplinary Center for Biotechnology Research at the University of Florida. Florida Agricultural Experiment Station Journal Series R-02399.

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Guy, C., Haskell, D., Neven, L. et al. Hydration-state-responsive proteins link cold and drought stress in spinach. Planta 188, 265–270 (1992). https://doi.org/10.1007/BF00216823

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