Skip to main content
SpringerLink
Log in

The projections of chemically identified nerve fibres in canine ileum

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

The projections of nerve fibres with immunoreactivity for the peptides enkephalin (ENK), gastrin-releasing peptide (GRP), neuropeptide Y (NPY), somatostatin (SOM), substance P (SP) and vasoactive intestinal peptide (VIP) were studied in canine small intestine by analysing the consequences of lesions of intrinsic and extrinsic nerves. Of peptides present in fibres supplying myenteric ganglia, GRP, SOM and VIP were in anally directed nerve pathways, whereas ENK and NPY were in orally directed pathways. Pathways ran for up to about 30 mm. SP fibres ran for short distances in both directions in the myenteric plexus. The circular muscle was supplied with ENK, NPY, SP and VIP fibres arising from the myenteric ganglia, whereas most mucosal SP and VIP fibres were deduced to arise from submucous ganglia. There were projections of fibres reactive for ENK, GRP, SOM, SP and VIP from myenteric ganglia to submucous ganglia. Antibodies to tyrosine hydroxylase were used to locate noradrenaline nerve fibres supplying the intestine; these fibres all disappeared when extrinsic nerves running through the mesentery to the small intestine were cut. It is deduced that there is an ordered pattern of projections of peptide-containing fibres in the canine intestine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Burks TF (1973) Mediation of 5-hydroxytryptamine and morphine stimulant actions in dog intestine. J Pharmacol Exp Ther 185:530–539

    Google Scholar 

  • Bury RW, Mashford ML (1977) Substance P: Its pharmacology and physiological roles. Aust J Exp Biol Med Sci 55:671–735

    Google Scholar 

  • Costa M, Furness JB (1982) Neuronal peptides in the intestine. Br Med Bull 38:347–352

    Google Scholar 

  • Costa M, Furness JB (1983a) Immunohistochemistry on whole mount preparations. In: Cuello AC (ed) Methods in neuroscience, vol. 3, Immunohistochemistry. Wiley, New York, pp 373–398

    Google Scholar 

  • Costa M, Furness JB (1983b) The origins, pathways and terminations of neurons with VIP-like immunoreactivity in the guinea- pig small intestine. Neuroscience 8:665–676

    Google Scholar 

  • Costa M, Furness JB, Elewellyn-Smith IJ, Davies B, Oliver J (1980a) An immunohistochemical study of the projections of somatostatin-containing neurons in the guinea-pig intestine. Neuroscience 5:841–852

    Google Scholar 

  • Costa M, Buffa R, Furness JB, Solcia EL (1980b) Immunohistochemical localization of polypeptides in peripheral autonomic nerves using whole mount preparations. Histochemistry 65:157–165

    Google Scholar 

  • Costa M, Furness JB, Llewellyn-Smith IJ, Cuello AC (1981) Projections of substance P neurons within the guinea-pig small intestine. Neuroscience 6:411–424

    Google Scholar 

  • Costa M, Furness JB, Yanaihara N, Yanaihara C, Moody TW (1984) Distribution and projections of neurons with immunoreactivity for both gastrin-releasing peptide and bombesin in the guinea-pig small intestine. Cell Tissue Res 235:285–293

    Google Scholar 

  • Costa M, Furness JB, Pullin CO, Bornstein J (1985) Substance P enteric neurons mediate non-cholinergic transmission to the circular muscle of the guinea-pig intestine. Naunyn-Schmiedeberg's Arch Pharmacol 328:446–453

    Google Scholar 

  • Costa M, Furness JB, Llewellyn-Smith IJ (1986) Histochemistry of the enteric nervous system. In: Johnson LR (ed) Physiology of the gastrointestinal tract. Raven Press, New York (in press)

    Google Scholar 

  • Daniel EE, Gonda T, Domoto T, Oki M, Yanaihara N (1982) The effects of substance P and met-enkephalin in dog ileum. Can J Physiol Pharmacol 60:830–840

    Google Scholar 

  • Daniel EE, Costa M, Furness JB, Keast JR (1985) Peptide neurons in the canine small intestine. J Comp Neurol 237:227–238

    Google Scholar 

  • Ekblad E, Ekman R, Håkanson R, Sundler F (1984) GRP neurones in the rat small intestine issue long anal projections. Regul Pept 9:279–287

    Google Scholar 

  • Fox JET, Daniel EE (1984) Receptor-type dependent opiate inhibition or excitation of canine gastrointestinal motility. Gastroenterology 86:1080

    Google Scholar 

  • Fox JET, Daniel EE (1985) Substance P: a potent inhibitor of the canine small intestine in vivo. Am J Physiol 250:G21-G27

    Google Scholar 

  • Fox JET, McDonald TJ (1984) Motor effects of gastrin-releasing peptide (GRP) and bombesin in the canine stomach and small intestine. Life Sci 35:1667–1673

    Google Scholar 

  • Fox JET, Daniel EE, Jury J, Robotham KH (1984) Stimulation of M1 muscarinic receptors by cholinergic agonists and peptides produces inhibition in canine small intestine. Gastroenterology 86:1081

    Google Scholar 

  • Fox JET, Daniel EE, McDonald TJ (1985) Peptidergic activation of muscarinic M1 inhibition in the canine small intestine in vivo. In: Lux G, Daniel EE (eds) Muscarinic receptor subtypes in gastrointestinal tract. Springer, Berlin Heidelberg New York, pp 67–75

    Google Scholar 

  • Furness JB, Costa M (1979) Projections of intestinal neurons showing immunoreactivity for vasoactive intestinal polypeptide are consistent with these neurons being the enteric inhibitory neurons. Neurosci Lett 15:199–204

    Google Scholar 

  • Furness JB, Costa M (1982a) Identification of gastrointestinal neurotransmitters. In: Bertaccini G (ed) Mediators and drugs in gastrointestinal motility I. Springer, Berlin Heidelberg New York pp 381–460

    Google Scholar 

  • Furness JB, Costa M (1982b) Neurons with 5-hydroxytryptaminelike immunoreactivity in the enteric nervous system: their projections in the guinea-pig small intestine. Neuroscience 7:341–349

    Article  CAS  PubMed  Google Scholar 

  • Furness JB, Costa M, Emson PC, Håkanson R, Moghimzadeh E, Sundler F, Taylor IL, Chance RE (1983a) Distribution, pathways and reactions to drug treatment of nerves with neuropeptide Y and pancreatic polypeptide-like immunoreactivity in the guinea-pig digestive tract. Cell Tissue Res 234:71–92

    Google Scholar 

  • Furness JB, Costa M, Miller RJ (1983b) Distribution and projections of nerves with enkephalin-like immunoreactivity in the guinea-pig small intestine. Neuroscience 8:653–664

    Google Scholar 

  • Furness JB, Llewellyn-Smith IJ, Bornstein JC, Costa M (1986) Neuronal circuitry in the enteric nervous system. Handbook of Chemical Neuroanatomy (in press)

  • Keast JR, Furness JB, Costa M (1984) The origins of peptide and norepinephrine nerves in the mucosa of the guinea-pig small intestine. Gastroenterology 86:637–644

    Google Scholar 

  • Llewellyn-Smith IJ, Furness JB, Murphy R, O'Brien PE, Costa M (1984) Substance P containing nerves in human small intestine — distribution, ultrastructure and characterization of the immunoreactive peptide. Gastroenterology 86:421–435

    Google Scholar 

  • Malmfors G, Leander S, Brodin E, Håkanson R, Holmin T, Sundler F (1981) Peptide-containing neurons intrinsic to the gut wall. An experimental study in the pig. Cell Tissue Res 214:225–238

    Google Scholar 

  • Pickel VM, Joh TH, Field PM, Becker C, Reis DJ (1975) Cellular localization of tyrosine hydroxylase in immunocytochemistry. J Histochem Cytochem 23:1–12

    Google Scholar 

  • Sakai Y, Daniel EE, Jury J, Fox JET (1984) Neurotensin inhibition of canine intestinal motility in vivo via α-adrenoceptors. Can J Physiol Pharmacol 62:403–411

    Google Scholar 

  • Sarna SK, Condon RE (1984) Morphine-initiated migrating myoelectric complexes in the fed state in dogs. Gastroenterology 86:662–669

    Google Scholar 

  • Schultzberg M, Hökfelt T, Nilsson G, Terenius L, Elfvin L-G, Lundberg JM, Brandt J, Elde R, Goldstein M (1980) Distribution of peptide and catecholamine neurons in the gastrointestinal tract of rat and guinea-pig: immunohistochemical studies with antisera to substance P, VIP, enkephalins, somatostatin, gastrin, neurotensin and dopamine-β-hydroxylase. Neuroscience 5:689–744

    Article  CAS  PubMed  Google Scholar 

  • Stefanini M, DeMartino C, Zamboni C (1967) Fixation of ejaculated spermatozoa for electron microscopy. Nature 216:173–174

    Google Scholar 

  • Sundler F, Håkanson R, Leander S, Uddman R (1982) Neuropeptides in the gut wall: Cellular and subcellular localization, topographic distribution, and possible physiological significance. In: Chan-Palay V, Palay SL (eds) Cytochemical methods in neuroanatomy. Alan R. Liss, Inc., New York pp 341–356

    Google Scholar 

  • Tange A (1983) Distribution of peptide-containing endocrine cells and neurons in the gastrointestinal tract of the dog: Immunohistochemical studies using antisera to somatostatin, substance P, vasoactive intestinal polypeptide, met-enkephalin, and neurotensin. Biomed Res 4:9–24

    Google Scholar 

  • Wilson AJ, Llewellyn-Smith IJ, Furness JB, Costa M (1986) The source of the nerve fibres forming the deep muscular and circular muscle plexuses in the guinea-pig small intestine. Cell Tissue Res (in press)

  • Yoshikawa H (1970) An experimental study on the sympathetic neuron chains using the fluorescence method for biogenic amines. Arch Histol Jpn 31:495–509

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Neurosciences, McMaster University, Hamilton, Ontario, Canada

    E. E. Daniel

  2. Department of Pathology, McMaster University, Hamilton, Ontario, Canada

    L. Belbeck

  3. Centre for Neuroscience, School of Medicine, Flinders University, Bedford Park, SA, Australia

    J. B. Furness & M. Costa

Authors
  1. E. E. Daniel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. B. Furness
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Costa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Belbeck
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Daniel, E.E., Furness, J.B., Costa, M. et al. The projections of chemically identified nerve fibres in canine ileum. Cell Tissue Res. 247, 377–384 (1987). https://doi.org/10.1007/BF00218319

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00218319

Key words

Search

Navigation