Summary
The appearance of the rat epididymal epithelium changed when it was perfused in vivo through the lumen with unphysiologically high sodium ion concentrations; dilatation of intercellular spaces (ICS) at threshold concentrations of 30mM-Na+ in the cauda and about 55mM-Na+ in the corpus was associated with absorption of water from the lumen. Despite the distended ICS, junctional complexes appeared intact, and their integrity was confirmed by the exclusion of luminal horseradish peroxidase (HRP) from the ICS, and by demonstrating that circulating [3H]inulin did not enter the lumen. Smooth ER and lipid droplets in the principal cells of the corpus epididymidis were well maintained, and the preservation of granular ER in principal cells of the cauda epididymidis lent morphological support to the continued secretion of protein in this segment. However, occasional distension or involution of inner Golgi cisternae was evident in principal cells after 3–6 h perfusion. In contrast to multivesicular bodies of principal cells, the apical and basal vacuoles characteristic of clear cells changed in size with different perfusing solutions. When low Na+ concentrations were perfused large translucent vacuoles were frequently found in the apical cytoplasm of clear cells in the corpus and cauda epididymidis, and filled vacuoles became larger and showed a decrease in content density in the cauda epididymidis. These large vacuoles were absent from tissue perfused with high Na+ concentrations. Normal pinocytotic activity of both cell types was demonstrated by perfusing HRP which was taken up by the normal route in principal cells, with some transfer to the Golgi cisternae. By far the most HRP was accumulated in clear cell vacuoles irrespective of the composition of the perfusing solution.
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References
Cooper TG (1980) The general importance of proteins and other factors in the transfer of steroids into the rat epididymis. Int J Androl 32:333–348
Cooper TG (1982) Secretion of inositol and glucose by the perfused rat cauda epididymidis. J Reprod Fert 64:373–379
Cooper TG, Brooks DE (1981) The entry of glycerol into the rat epididymis and its utilization by epididymal spermatozoa. J Reprod Fert 61:163–169
Cooper TG, Waites GMH (1979a) Factors affecting the entry of testosterone into the lumen of the cauda epididymidis of the anaesthetized rat. J Reprod Fert 56:165–174
Cooper TG, Waites GMH (1979b) Investigation by luminal perfusion of the transfer of compounds into the epididymis of the anaesthetized rat. J Reprod Fert 56:159–164
Cooper TG, Yeung CH (1980) Epithelial structure of the rat cauda epididymidis after luminal perfusion. Int J Androl 3:367–374
Diamond JM, Bossert WH (1967) Standing-gradient osmotic flow. A mechanism for coupling of water and solute transport in epithelia. J Gen Physiol 50:2061–2083
DiBona DR (1972) Passive intercellular pathway in amphibian epithelia. Nature New Biol 238:179–181
DiBona DR, Civan MM (1970) The effect of smooth muscle on the intercellular spaces in toad urinary bladder. J Cell Biol 46:235–244
Flickinger CJ, Howards SS, English HE (1978) Ultrastructural differences in efferent ducts and several regions of the epididymis of the hamster. Am J Anat 152:557–586
Friend DS (1969) Cytochemical staining of multivesicular body and Golgi vesicles. J Cell Biol 41:269–279
Hamilton DW (1975) Structure and function of the epithelium lining the ductuli efferentes, ductus epididymidis, and ductus deferens in the rat. In: Hamilton DW, Greep RO (eds) Handbook of Physiology: Endocrinology, Vol 5. Am Physiol Soc, Washington DC, pp 259–303
Hamilton DW, Fawcett DW (1970) In vitro synthesis of cholesterol and testosterone from acetate by the rat epididymis and vas deferens. Proc Soc Exp Biol Med 133:693–695
Hartree EF (1972) Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem 48:422–427
Herzog V, Farquhar MG (1977) Luminal membrane retrieved after exocytosis reaches most Golgi cisternae in secretory cells. Proc Natl Acad Sci USA 74:5073–5077
Hinton BT, Setchell BP (1980) Concentrations of glycerophosphocholine, phosphocholine and free organic phosphate in the luminal fluid of the rat testis and epididymis. J Reprod Fert 58:401–406
Hoffer AP, Hamilton DW, Fawcett DW (1973) The ultrastructure of the principal cells and intraepithelial leucocytes in the initial segment of the rat epididymis. Anat Rec 175:169–202
House CP (1974) Permeability characteristics of epithelia. In: Water transport in cells and tissues. E Arnold Ltd, London, p 318
Jones R, Glover TD (1975) Interrelationships between spermatozoa, the epididymis and epididymal plasma. In: Duckett JG, Racey PA (eds) Biol J Linnean Soc 7, Suppll, The biology of the male gamete, pp 367–384
Jones R, Hamilton DW, Fawcett DW (1979) Morphology of the epithelium of the extratesticular rete testis, ductuli efferentes and ductus epididymidis. Am J Anat 156:373–399
Kopečný V, Péch V (1977) An autoradiographic study of macromolecular synthesis in the epithelium of the ductus epididymidis in the mouse. II. Incorporation of L-fucose-1-3H. Histochem 50:229–238
Lea OA, Petrusz P, French FS (1978) Purification and localization of acidic epididymal glycoprotein (AEP): A sperm coating protein secreted by the rat epididymis. Int J Androl Suppl 2:592–607
Levine N, Marsh DJ (1971) Micropuncture studies of the electrochemical aspects of fluid and electrolyte transport in individual seminiferous tubules, the epididymis and the vas deferens in rats. J Physiol 213:557–570
Loeschke K, Bentzel CJ, Csaky TZ (1970) Asymmetry of osmotic flow in frog intestine: functional and structural correlation. Am J Physiol 218:1723–1731
Møllgard K, Rostgaard J (1978) Morphological aspects of some sodium transporting epithelia suggesting a transcellular pathway via elements of endoplasmic reticulum. J Memb Biol 1978 special issue: Epithelia as hormone and drug receptors 77–89
Møllgard K, Saunders NR (1975) Complex tight junctions of epithelial and of endothelial cells in early foetal brain. J Neurocytol 4:453–468
Moore HDM, Bedford JM (1979) The differential absorptive activity of epithelial cells of the rat epididymis before and after castration. Anat Rec 193:313–327
Oschman JL, Wann BJ, Gupta BL (1974) Cellular basis of water transport. Symp Soc Expl Biol 28:305–350
Smulders AP, Tormey JMcD, Wright EM (1972) The effect of osmotically induced water flows on the permeability and ultrastructure of the rabbit gallbladder. J Memb Biol 7:164–197
Waites GMH (1977) Fluid secretion. In: Johnson AD, Gomes WR (eds) The Testis, Vol 4. Academic Press, New York, pp 91–123
Wenstrom JC, Hamilton DW (1980) Dolichol concentration and biosynthesis in rat testis and epididymis. Biol Reprod 23:1054–1059
Wong PYD, Yeung CH (1978) Absorptive and secretory functions in the perfused rat cauda epididymidis. J Physiol 275:13–26
Wong PYD, Au CL, Ngai HK (1978) Electrolyte and water transport in rat epididymis: its possible role in sperm maturation. Int J Androl Suppl 2:608–628
Wong PYD, Au CL, Ngai HK (1979) Some characteristics of salt and water transport in the rat epididymis. In: Fawcett DW, Bedford JM (eds) The Spermatozoon. Urban and Schwarzenburg, Baltimore, pp 57–63
Wong PYD, Tsang AYE, Lee WM (1980) Effect of intraluminal ion concentrations on the secretion of the rat cauda epididymidis in vivo. Pflügers Arch 387:61–66
Wong YC, Wong PYD, Yeung CH (1978) Ultrastructural correlation of water reabsorption in isolated rat cauda epididymidis. Experientia 34:485–487
Yeung CH, Cooper TG, Waites GMH (1980) Carnitine transport into the perfused epididymis of the rat: regional differences, stereo-specificity, stimulation by choline and the effect of other luminal factors. Biol Reprod 23:294–304
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Yeung, C.H., Cooper, T.G. Ultrastructure of the perfused rat epididymis: Effect of luminal sodium ion concentration. Cell Tissue Res. 226, 407–425 (1982). https://doi.org/10.1007/BF00218369
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DOI: https://doi.org/10.1007/BF00218369