Summary
In the present study an attempt was made to demonstrate melatonin in the rat pineal gland by means of immunohistochemistry. The anti-body used was raised against 5-methoxy-N-acetyltryptophan which is chemically similar to melatonin. Specific fluorescence was demonstrable only in pineals from rats killed during the night, when melatonin formation is high. It was restricted to parenchymal cells lying in a marginal zone of the organ. These results are discussed in relation to a subdivision of the pineal parenchyma into cortical and medullary areas.
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References
Arendt, J., Paunier, L., Sizonenko, P.C.: Melatonin radioimmunoassay. J. clin. Endocr. 40, 347–350 (1975)
Axelrod, J., Wurtman, R.J., Snyder, S.H.: Control of hydroxyindole O-methyltransferase activity in the rat pineal gland by environmental lighting. J. biol. Chem. 240, 949–954 (1965)
Blumfield, M.G., Tapp, E.: Measurements of pineal parenchymal cells and their nuclei in the albino rat at different ages. Acta morph. neerl.-scand. 8, 1–8 (1970)
Bowie, E.P., Herbert, D.C.: Immunocytochemical evidence for the presence of arginine vasotocin in the rat pineal gland. Nature (Lond.) 261, 66 (1976)
Brown, G.M.: Personal communication
Bubenik, G.A., Brown, G.M., Grota, L.J.: Immunohistological investigations of N-acetylserotonin in the rat cerebellum after parachlorophenylalanine. Experientia (Basel) 32, 579–581 (1976a)
Bubenik, G.A., Brown, G.M., Grota, L.J.: Differential localization of melatonin and N-acetylserotonin in CNS and the Harderian gland. Anat. Rec. 184, 365–366 (1976b)
Bubenik, G.A., Brown, G.M., Uhlir, I., Grota, L.J.: Immunohistological localization of N-acetylindole-alkylamines in pineal gland, retina and cerebellum. Brain Res. 81, 233–242 (1974)
Cheesman, D.W.: Structure elucidation of a gonadotropin inhibiting substance from the bovine pineal gland. Biochim. biophys. Acta (Amst.) 207, 247–253 (1970)
Coons, A.H.: The application of fluorescent antibodies to the study of naturally occurring antigens. Ann. N.Y. Acad. Sci. 69, 658–662 (1957)
Dafny, N., McClung, R., Strada, S.J.: Neurophysiological properties of the pineal body. 1. Field potentials. Life Sci. 16, 611–620 (1975)
Grota, L.J., Brown, G.M.: Antibodies to indolealkylamines: serotonin and melatonin. Canad. J. Biochem. 52, 196–202 (1974)
Haulica, I., Branisteanu, D.D., Rosca, V., Stratone, A., Berbeleu, V., Balan, G., Ionescu, L.: A reninlike activity in pineal gland and hypophysis. Endocrinology 96, 530–532 (1975)
Klein, D.C., Weller, J.L.: Indole metabolism in the pineal gland: a circadian rhythm in N-acetyl-transferase. Science 169, 1093–1095 (1970)
König, A., Meyer, A.: Tagesperiodische Schwankungen einer antidiuretischen Aktivität aus der Epiphysis cerebri ausgewachsener männlicher Ratten. Naturwissenschaften 54, 93 (1967)
König, A., Meyer, A.: The effect of continuous illumination on the circadian rhythm of the antidiuretic activity of the rat pineal. J. Interdiscipl. Cycle Res. 2, 255–262 (1971)
Levine, L., Riceberg, L.J.: Radioimmunoassay for melatonin. Res. Commun. Chem. Path. Pharmacol. 10, 693–702 (1975)
McClung, R., Dafny, N.: Neurophysiological properties of the pineal body. II. Single unit recording. Life Sci. 16, 621–628 (1975)
Pang, S.F., Brown, G.M., Grota, L.J., Rodman, R.L.: Radioimmunoassay of melatonin in pineal glands, Harderian glands, retinas and sera of rats or chickens. Fed. Proc. 35, 691 (1976)
Pavel, S.: Endocrine functions of arginine vasotocin from mammalian pineal gland. Gen. comp. Endocr. 9, 481 (1967)
Quay, W.B.: Cytochemistry of pineal lipids in rat and man. J. Histochem. Cytochem. 5, 145–153 (1957)
Quay, W.B.: Pineal blood content and its experimental modification. Amer. J. Physiol. 195, 391–395 (1958)
Quay, W.B.: Circadian rhythm in rat pineal serotonin and its modifications by estrous cycle and photoperiod. Gen. comp. Endocr. 3, 473–479 (1963)
Quay, W.B., Renzoni, A.: Twenty-four-hour rhythms in pineal mitotic activity and nuclear and nucleolar dimensions. Growth 30, 315–324 (1966)
Rosenbloom, A.A., Fisher, D.A.: Radioimmunoassayable AVT and AVP in adult mammalian brain tissue: Comparison of normal and Brattleboro rats. Neuroendocrinology 17, 345–361 (1975)
Rudeen, P.K., Reiter, R.J., Vaughan, M.K.: Pineal serotonin-N-acetyltransferase activity in four mammalian species. Neurosci. Letters 1, 225–229 (1975)
White, W.F., Hedlund, M.T., Weber, G.F., Rippel, R.H., Johnson, E.S., Wilber, J.F.: The pineal gland: a supplemental source of hypothalamic-releasing hormones. Endocrinology 94, 1422–1426 (1974)
Wilkinson, M., Arendt, J., de Ziegler, D., Ruf, K.B.: Changes in sensitivity of pinealocyte β-receptors during precocious sexual maturation or after postnatal sterilization with estrogen in the female. Ann. Biol. Bioch. Biophys. 16, 457–464 (1976)
Wurtman, R.J., Axelrod, J., Kelly, D.E.: The pineal. New York-London: Academic Press 1968
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Supported by a grant of the Deutsche Forschungsgemeinschaft (VO 135/4) within the “Schwerpunktprogramm Neuroendokrinologie”
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Freund, D., Arendt, J. & Vollrath, L. Tentative immunohistochemical demonstration of melatonin in the rat pineal gland. Cell Tissue Res. 181, 239–244 (1977). https://doi.org/10.1007/BF00219983
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DOI: https://doi.org/10.1007/BF00219983