Summary
Preimplantation-stage embryos were transferred to the anterior eye chamber of recipient rats and guinea-pigs. After implantation had occurred the influence of the embryo on the iris vasculature was examined ultrastructurally. In both species, the earliest effect of embryonic implantation was an increased stromal oedema. Under increasing embryonic influence the vascular endothelial cells showed an increased number of projections into the vascular lumen, while in the rat, endothelial projections were also found pushing back into the basement membrane. In the rat, the endothelium became very irregular in thickness prior to complete disintegration and loss during more advanced stages of implantation. Rat embryonic trophoblast was found invading iris vasculature, particularly in areas where the iridial endothelium was partially or completely missing. Other cells in the iris, including the stroma, appeared to be less affected. In the guinea-pig, however, trophoblast cells appeared to be capable of invading the vasculature by displacing endothelial cells that still appeared morphologically normal.
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Abrahamsohn P, Lundkvist Ö, Nilsson (1983) Ultrastructure of the endometrial blood vessels during implantation of the rat blastocyst. Cell Tissue Res 229:269–280
Brandon JM, Wallis RM (1977) Effect of mepyramine, a histamine H1, and buramide, a histamine H2 receptor antagonist, on ovum implantation in the rat. J Reprod Fert 50:251–254
Deansly R (1971) The differentiation of the decidua at ovo-implantation in the guinea-pig contrasted with that of the traumatic deciduoma. J Reprod Fertil 26:91–97
Fawcett DW (1950) The development of mouse ova under the capsule of the kidney. Anat Rec 108:71–91
Fawcett DW, Wislocki GB, Waldo CM (1947) The development of mouse ova in the anterior chamber of the eye and in the abdominal cavity. Am J Anat 81:413–443
Finn CA, Bredl JCS (1973) Studies on the development of the implantation reaction in the mouse uterus: influence of actinomycin D. J Reprod Fertil 34:247–252
Finn CA, Porter DG (1975) Implantation. In: Finn CA (ed) The Uterus. Elek Science, London, pp 57–73
Grisham MB, Hernandez LA, Granger DN (1986) Xanthine oxidase and neutrophil infiltration in intestinal ischemia. Am J Physiol 251:G567-G574
Horan AH (1971) The suppression of inflammatory edema at the nidation site by sodium salicylate and nitrogen mustard in the rat. Fertil Steril 22:392–397
Kennedy TG, Armstrong DT (1981) The role of prostaglandins in endometrial vascular changes at implantation. In: Glasser SR, Bullock DW (eds) Cellular and molecular aspects of implantation. Plenum Press, New York London, pp 349–363
Kirby DRS (1960) Development of mouse eggs beneath the kidney capsule. Nature 187:707–708
Kirby DRS (1963a) The development of mouse blastocysts transplanted to the scrotal and cryptorchid testis. J Anat 97:119–130
Kirby DRS (1963b) Development of the mouse blastocyst transplanted to the spleen. J Reprod Fertil 5:1–12
Lundkvist Ö, Nilsson BO (1982) Endometrial ultrastructure in the early uterine response to blastocysts and artificial deciduogenic stimuli in rats. Cell Tissue Res 225:355–364
Owers NO, Blandau RJ (1971) Proteolytic activity of the rat and guinea-pig blastocyst in vitro. In: Blandau RJ (ed) Biology of the blastocyst. University Chicago Press, Chicago London, pp 207–224
Psychoyos A (1973) Endocrine control of egg implantation. In: Greep RO, Astwood EG, Geiger SR (eds) Handbook of physiology, Section 7, Volume II, Part 2. American Physiological Society, Washington, pp 187–215
Rogers PAW, Murphy CR, Gannon BJ (1982) Changes in the spatial organization of the uterine vasculature during implantation in the rat. J Reprod Fertil 65:211–214
Rogers PAW, Murphy CR, Rogers AW, Gannon BJ (1983) Capillary patency and permeability in the endometrium surrounding the implanting rat blastocyst. Int J Microcirc: Clin Exp 2:241–249
Rogers PAW, Macpherson AM, Beaton LA (1988) Embryo implantation in the anterior chamber of the eye: effects on uterine allografts and the microvasculature. Ann NY Acad Sci (In press)
Runner MN (1947) Development of mouse eggs in the anterior chamber of the eye. Anat Rec 98:1–17
Szalay J, Nunziata B, Henkind P (1975) Permeability of iridial blood vessels. Exp Eye Res 21:531–543
Wedmore CV, Williams TJ (1981) Control of vascular permeability by polymorphonuclear leukocytes in inflammation. Nature 289:646–650
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Rogers, P.A.W., Macpherson, A.M. & Beaton, L.A. Vascular response in a non-uterine site to implantation-stage embryos in the rat and guinea-pig: in vivo and ultrastructural studies. Cell Tissue Res. 254, 217–224 (1988). https://doi.org/10.1007/BF00220037
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DOI: https://doi.org/10.1007/BF00220037