Summary
Acidophilic cells occur in the epidermis of several species of salmonid fish, although their abundance fluctuates considerably between individuals within the same population and at different times during the life cycle. The histology, histochemistry and ultrastructure of an acidophilic, granular celltype in the epidermis of the brown trout, Salmo trutta L., is described. At the light microscope level this cell type is easily distinguished from the large, mucus-secreting, epidermal goblet cells by its acidophilic, proteinaceous secretion. At the ultrastructural level this secretion consists of membrane-bound granules formed by the very active Golgi region. It is argued that the acidophilic, granular cell is not a transformed blood cell but constitutes a normal epidermal component of the brown trout. Possible roles of this cell in the function(s) of the epidermis are discussed.
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References
Banerjee TK, Agarwal SK, Rai AK, Mittal AK (1976) Histochemical localisation of alkaline phosphatase, acid phosphatase, and succinic dehydrogenase activities in the epidermis of the freshwater teleost, Amphipnous cuchia (Hamilton) (Symbranchiformes, Pisces). Mikroskopie 32:294–300
Bhatti HK (1938) The integument and dermal skeleton of siluroidea. Trans Zool Soc Lond 24:1–102
Blaxall PC, Daisley KW (1973) Routine haematological methods for use with fish blood. J Fish Biol 5:771–781
Bradshaw CM, Richard AS, Sigel MM (1971) IgM antibodies in fish mucus. Proc Soc Exp Biol Med 136:1122–1124
Brissie RM, Spicer SS, Hall BJ, Thompson NT (1974) Ultrastructural staining of thin sections with iron hematoxylin. J Histochem Cytochem 22:895–907
Bullock AM, Roberts RJ (1975) The dermatology of marine teleost fish. 1. The normal integument. Oceanogr Mar Biol Ann Rev 13:383–411
Celada M, de Paoli AM (1962) Contributo alla conoscenza delle cellule albuminose (Schneider) cutanee di Torpedo ocellata Raf. Riv Istochim Norm Pat 8:411–416
Ellis AE (1977) The leucocytes of fish: A review. J Fish Biol 11:453–491
Fletcher TC, Grant PT (1968) Glycoproteins in the external mucous secretions of the plaice, Pleuronectes platessa, and other fishes. Proc Biochem Soc 12P. In Biochem J 106
Fletcher TC, Grant PT (1969) Immunoglobulins in the serum and mucous of the plaice (Pleuronectes platessa). Proc Biochem Soc 65 P. In Biochem J 115
Harris JE, Hunt S (1975a) The fine structure of the epidermis of two species of salmonid fish, the atlantic salmon (Salmo salar L.) and the brown trout (Salmo trutta L.). 1. General organization and filament-containing cells. Cell Tissue Res 157:553–565
Harris JE, Hunt S (1975b) The fine structure of the epidermis of two species of salmonid fish, the atlantic salmon (Salmo salar L.) and the brown trout (Salmo trutta L.). 11. Mucous cells. Cell Tissue Res 163:535–543
Jakowska S (1963) Mucous secretion in fish — A note. Ann NY Acad Sci 106:458–462
Lane BP, Europa DL (1965) Differential staining of ultrathin sections of epon-embedded tissues for light microscopy. J Histochem Cytochem 13:579–582
Lillie RD, Fullmer HM (1976) Histopathologic technic and practical histochemistry. McGraw-Hill New York: 4th ed.
Logan VH, Odense PH (1974) The integument of the ocean sunfish (Mola mola L.) (Plectognathi) with observations on the lesions from two ectoparasites, Capsala mastinierei (Trematoda) and Philorthagoriscus serratus (Copepoda). Cand J Zool 52:1039–1045
Mittal AK, Agarwal SK (1977) Histochemistry of the unicellular glands in relation to their physiological significance in the epidermis of Monopterus cuchia (Symbranchiformes, Pisces). J Zool, Lond 182:429–439
Mittal AK, Banerjee TK (1975) Histochemistry and the structure of the skin of a murrel, Channa striata (Bloch, 1797) (Channiformes, channidae). 1. Epidermis. Cand J Zool 53:833–843
Mittal AK, Banerjee TK (1976) Functional organization of the skin of the ‘Green-Puffer-Fish’ Tetraodon fluviatilis (Ham.-Buck.) (Tetraodontidae, Pisces). Zoomorphologie 84:195–209
Mittal AK, Munshi JSD (1971) A comparative study of the structure of the skin of certain air-breathing fresh-water teleosts. J Zool, Lond 163:515–532
Mittal AK, Munshi JSD (1974) On the regeneration and repair of superficial wounds in the skin of Rita rita (Ham.) (Bagridae, Pisces). Acta Anat 88:424–442
Mittal AK, Agarwal SK, Banerjee TK (1976) Protein and carbohydrate histochemistry in relation to the keritinization in the epidermis of Barbus sophur (Cyprinidae, Pisces). J Zool, Lond 179:1–17
Mittal AK, Whitear M, Bullock AM (1980) Sacciform cells in teleost fish. In press
Nigrelli RF (1935) On the effect of fish mucous on Epibdella melleni, a monogenetic trematode of marine fishes. J Parasitol 21:438
Pearse AGE (1968) Histochemistry theoretical and applied. Little, Brown and Co. Boston. 3rd ed Vol. 1
Pickering AD (1974) The distribution of mucous cells in the epidermis of the brown trout Salmo trutta (L.) and the char Salvelinus alpinus (L.). J Fish Biol 6:111–118
Pickering AD (1977) Seasonal changes in the epidermis of the brown trout Salmo trutta (L.). J Fish Biol 10:561–566
Pickering AD, Macey DJ (1977) Structure, histochemistry and the effect of handling on the mucous cells of the epidermis of the char Salvelinus alpinus (L.). J Fish Biol 10:505–512
Pickering AD, Richards RH (1980) Factors influencing the structure, function and biota of the salmonid epidermis. Proc R Soc Edin B. In press
Porcelli F, Massari S (1969) Dati istochimici sulle cellule mucipare cutanee durante lo sviluppo della trota. Boll Soc Ital Biol Sper 45:1273–1276
Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212
Roberts RJ, Bullock AM (1976) The dermatology of marine teleost fish. II. Dermatopathology of the integument. Oceanogr Mar Biol Ann Rev 14:227–246
Roberts RJ, Shearer WM, Elson KGR, Munro ALS (1970) Studies on ulcerative dermal necrosis of salmonids. 1. The skin of the normal salmon head. J Fish Biol 2:223–229
Roberts RJ, Young H, Milne JA (1971) Studies on the skin of plaice (Pleuronectes platessa L.). 1. The structure and ultrastructure of normal plaice skin. J Fish Biol 4:87–98
Sato M (1967) Histological and histochemical observations on the eosinophilic vesicles found in the head epidermis of Blepsias cirrhosus (Pallas). A preliminary report. Arch Histol Jpn 28:377–382
Schwerdtfeger WK, Bereiter-Hahn J (1978) Transient occurrence of chloride cells in the abdominal epidermis of the guppy, Poecilia reticulata Peters, adapted to sea water. Cell Tissue Res 191:463–471
Stoklosowa S (1966) Sexual dimorphism in the skin of sea-trout, Salmo trutta. Copeia No 3. 613–614
Stoklosowa S (1970) Further observations on the sexual dimorphism in the skin of Salmo trutta trutta in relation to sexual maturity. Copeia No 2. 332–339
Zaccone G (1979) Histochemistry of keratinization and epithelial mucins in the skin of Muraena helena (L.). Cell Mol Biol 24:37–50
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Blackstock, N., Pickering, A.D. Acidophilic granular cells in the epidermis of the brown trout, Salmo trutta L.. Cell Tissue Res. 210, 359–369 (1980). https://doi.org/10.1007/BF00220194
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DOI: https://doi.org/10.1007/BF00220194