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Immunocytochemical identification of an LHRH-producing system originating in the preoptic nucleus of the duck

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Summary

A fluorescent technique applying specific LHRH and vasotocin antisera was used for the immunocytochemical localization of the respective neurosecretory systems in the hypothalamus of gonadectomized, testosteronetreated and/or serotonin injected male domestic ducks. An immunoreactive (IR) LHRH-producing system, with perikarya located in the preoptic nucleus, could be traced through the ventral hypothalamus down to the external layer of the rostral and caudal ME, in close vicinity to the hypophysial portal system. An IR-vasotocin system originating in the paraventricular and supraoptic nuclei ran through the ventral hypothalamus, but terminated in (i) the external layer of the rostral ME, and (ii) in the posterior lobe of the hypophysis.

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References

  • Assenmacher, I.: Recherches sur le contrôle hypothalamique de la fonction gonadotrope préhy-pophysaire chez le Canard. Arch. Anat. micr. Morph. exp. 47, 447–572 (1958)

    Google Scholar 

  • Barry, J.: Characterization and topography of LH-RH neurons in rabbit. Neurosci. Letters 3, 287–291 (1976a)

    Google Scholar 

  • Barry, J.: Characterization and topography of LH-RH neurons in the human brain. Neurosci. Letters 2, 201–205 (1976b)

    Google Scholar 

  • Barry, J., Carette, B.: Immunofluorescence study of LRF neurons in primates Cell Tiss. Res. 164, 163–178 (1975)

    Google Scholar 

  • Barry, J., Dubois, M.P.: Etude en immunofluorescence des structures hypothalamiques à compétence gonadotrope. Ann. Endocr. (Paris) 34, 735–742 (1973)

    Google Scholar 

  • Barry, J., Dubois, M.P.: Immunofluorescence study of LRF-producing neurons in the cat and the dog. Neuroendocrinology 18, 290–298 (1975)

    Google Scholar 

  • Barry, J., Dubois, M.P., Poulain, P.: LRF producing cells of the mammalian hypothalamus. Z. Zellforsch. 146, 351–366 (1973)

    Google Scholar 

  • Blähser, S., Simon, E.: Immunocytochemical studies on vasotocinergic and mesotocinergic neurons in the hypothalamus of osmotically stressed domestic mallards. p. 12. Abstr. 9th Conf. Europ. Comp. Endocrinol. Giessen 1977

  • Bons, N.: Mise en évidence au microscope électronique, de terminaisons nerveuses d'origine rétinienne dans l'hypothalamus antérieur du Canard. C.R. Acad. Sci. (Paris) 278, 319–321 (1974)

    Google Scholar 

  • Bons, N.: Retinohypothalamic pathway in the duck (Anas platyrhynchos). Cell Tiss. Res. 168, 343–360 (1976)

    Google Scholar 

  • Bons, N., Assenmacher, I.: Présence de fibres rétiniennes dégénérées dans la région hypothalamique supra-optique du Canard après section d'un nerf optique. C.R. Acad. Sci. (Paris) 269, 1535–1538 (1969)

    Google Scholar 

  • Bons, N., Kerdelhué, B., Assenmacher, I.: Evidence for LH-RH containing neurons in the anterior hypothalamus of the duck (Anas platyrhynchos); an immunofluorescence study, p. 13. Abstr. 9th Conf. Europ. Comp. Endocr. Giessen 1977a

  • Bons, N., Kerdelhué, B., Assenmacher, I.: Présence de neurones élaborateurs de LH-RH dans l'hypothalamus du Canard (Anas platyrhynchos) C.R. Acad. Sci. (Paris) 285, 1327–1330 (1977b)

    Google Scholar 

  • Bugnon, C., Block, B., Fellman, D.I.: Mise en évidence cytoimmunologique de neurones à LH-RH chez le foetus humain. C.R. Acad. Sci. (Paris) 282, 1625–1628 (1976)

    Google Scholar 

  • Calas, A., Kerdelhué, B., Assenmacher, I., Jutisz, M.: Les axones à LH-RH de l'éminence médiane. Mise en évidence chez le Canard par une technique immunocytochimique. C.R. Acad. Sci. (Paris) 277, 2765–2768 (1973)

    Google Scholar 

  • Calas, A., Kerdelhué, B., Assenmacher, I., Jutisz, M.: Les axones à LH-RH de l'éminence médiane. Etude ultrastructurale chez le Canard par une technique immunocytochimique. C.R. Acad. Sci. (Paris) 278, 2557–2560 (1974)

    Google Scholar 

  • Calas, A., Kerdelhué, B., Assenmacher, I., Jutisz, M.: Exploration immunocytochimique de l'éminence médiane du Canard avec des I.S. contre LH-RH et la vasotocine, couplées avec une -G de cobaye. J. Physiol. (Paris) 70, 9B (1975)

  • Crosby, E.C., Showers, M.J.: Comparative anatomy of the preoptic and hypothalamic areas. In: The hypothalamus (W. Haymaker, E. Anderson, W.J.H. Nauta, eds.), pp. 61–135. Springfield, Ill.: Ch. C. Thomas 1969

    Google Scholar 

  • De Reviers, M., Dubois, M.P.: Binding of synthetic LHRF antibodies in the ME of the cockerel. Horm. Metab. Res. 6, 494 (1974)

    Google Scholar 

  • Follett, B.K.: The neuroendocrine regulation of gonadotropin secretion in avian reproduction. In: Breeding biology of birds (D.S. Farner, ed.), pp. 209–243. Washington D.C.: National Academy of Sciences 1973

    Google Scholar 

  • Gabrion, J., Kerdelhué, B., Alonso, G., Bosler, O., Assenmacher, I., Calas, A.: Vasotocin immunoreactive neurons in the hypothalamus of some non-mammalian vertebrates. In: Evolutionary aspects of neuroendocrinology, Leningrad 1976 (W. Bargmann et al., ed.). Berlin-Heidelberg-New York: Springer 1977 (in press)

    Google Scholar 

  • Goossens, N., Blähser, S., Oksche, A., Vandesande, F., Dierickx, K.: Immunocytochemical investigation of the hypothalamo-neurohypophysial system in birds. Cell Tiss. Res. 184, 1–13 (1977)

    Google Scholar 

  • Hartwig, H.G.: Electron microscopic evidence for a retinohypothalamic projection to the suprachiasmatic nucleus of Passer domesticus. Cell Tiss. Res. 153, 89–99 (1974)

    Google Scholar 

  • Leonardelli, J., Poulain, P., Tramu, G., Dubois, M.P.: Effects of exogenous serotonin on LH-RH secreting neurons in the guinea pig as revealed by immunofluorescence. Neuroendocrinol. 15, 69–72 (1974)

    Google Scholar 

  • Mazucca, M.: Existence de neurones à caractères neuroglandulaires dans l'aire préoptique du cobaye. Etude préliminaire en microscopie électronique. Ann. Endocr. (Paris) 36, 273–274 (1975)

    Google Scholar 

  • McNeil, T.H., Koslowski, G.S., Abel, J.H., Jr., Zimmerman, E.A.: Neurosecretory pathways in the mallard duck (Anas platyrhynchos) brain: localization by aldehyde fuchsin and immunoperoxydase techniques for neurophysin (NP) and gonadotropin releasing hormone (Gn-RH). Endocrinol. 99, 1323–1332 (1976)

    Google Scholar 

  • Meier, R.E.: Autoradiographic evidence for a direct retino-hypothalamic projection in the avian brain. Brain Res. 53, 417–421 (1973)

    Google Scholar 

  • Naik, D.V.: Immunoreactive LH-RH neurons in the hypothalamus identified by light and fluorescent microscopy. Cell Tiss. Res. 157, 423–436 (1975)

    Google Scholar 

  • Oliver, J., Baylé, J.D.: Photically evoked potentials in the gonadotropic areas of the quail hypothalamus. Brain Res. 64, 103–121 (1973)

    Google Scholar 

  • Sétáló, G., Vigh, S., Schally, A.V., Arimura, A., Flerkó, B.: LH-RH containing neural elements in the rat hypothalamus. Endocrinol. 96, 135–142 (1975)

    Google Scholar 

  • Sétáló, G., Vigh, S., Schally, A.V., Arimura, A., Flerkó, B.: Immunohistological study of the origin of LH-RH containing nerve fibres of the rat hypothalamus. Brain Res. 103, 597–602 (1976)

    Google Scholar 

  • Silverman, A.J.: Distribution of luteinizing hormone releasing hormone (LH-RH) in the guinea pig brain. Endocrinol. 99, 30–46 (1976)

    Google Scholar 

  • Silverman, A.J., Antunes, J.L., Ferin, M., Zimmerman, E.A.: The distribution of luteinizing hormonereleasing hormone (LHRH) in the hypothalamus of the rhesus monkey. Light microscopic study using immunoperoxydase technique. Endocrinol. 101, 134–142 (1977)

    Google Scholar 

  • Tixier-Vidal, A., Assenmacher, I.: Action de la métopirone sur la préhypophyse du Canard mâle. Essai d'identification des cellules corticotropes. C.R. Soc. Biol. (Paris) 157, 1350 (1963)

    Google Scholar 

  • Zimmerman, E.A., HSU, K.C., Ferin, M., Koslowski, G.P.: Localization of gonadotropin releasing hormone (Gn-RH) in the hypothalamus of the mouse by immunoperoxydase technique. Endocrinology 95, 1–8(1974)

    Google Scholar 

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Dr. B. Kerdelhué, Laboratoire des Hormones polypeptidiques du CNRS, F-91190 Gif-sur-Yvette, France

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Bons, N., Kerdelhué, B. & Assenmacher, I. Immunocytochemical identification of an LHRH-producing system originating in the preoptic nucleus of the duck. Cell Tissue Res. 188, 99–106 (1978). https://doi.org/10.1007/BF00220517

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