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Anthocyanin pathway in rice (Oryza sativa L): identification of a mutant showing dominant inhibition of anthocyanins in leaf and accumulation of proanthocyanidins in pericarp

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Abstract

The present study has surveyed a collection of indica rice (Oryza sativa) lines for tissue-specific anthocyanin pigmentation pattern, which has also been used for a genetically meaningful classification. This classification helped predict probable genotypes of rice lines and, in the process, a leaf blade-specific dominant inhibitor of pigmentation (Ilb) was predicted and its presence later confirmed in two lines. We ascribe most tissue-specific accumulation of anthocyanins to the presence of a different set of Pl alleles. Cyanidin, as a major pigment, and peonidin, as a minor pigment, were detected in purple-pigmented tissues. Further, the floral organ-derived tissues always contained a higher level of anthocyanins and, correspondingly, a relatively increased proportion of peonidin. One line, N22B, with a brown pericarp was identified and shown to accumulate proanthocyanidins, but with no anthocyanins, in the pericarp. We propose that the accumulation of proanthocyanidins is due to a block in the anthocyanin biosynthetic pathway in rice at the anthocyanidin synthase-mediated conversion of leucoanthocyanidin to anthocyanidin.

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Author notes

  1. V. S. Reddy

    Present address: Centre For Applied Plant Molecular Biology-I, Institut für Allgemeine Botanik, University of Hamburg, Ohnhorststrasse 18, D-22609, Hamburg, Germany

Authors and Affiliations

  1. Plant Molecular Genetics Laboratory, Departement of Plant Sciences, School of Life Sciences, University of Hyderabad, 500134, AP, India

    V. S. Reddy, S. Dash & A. R. Reddy

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  1. V. S. Reddy
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  2. S. Dash
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  3. A. R. Reddy
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Communicated by F. Salamini

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Reddy, V.S., Dash, S. & Reddy, A.R. Anthocyanin pathway in rice (Oryza sativa L): identification of a mutant showing dominant inhibition of anthocyanins in leaf and accumulation of proanthocyanidins in pericarp. Theoret. Appl. Genetics 91, 301–312 (1995). https://doi.org/10.1007/BF00220892

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  • DOI: https://doi.org/10.1007/BF00220892

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