Summary
Anoxia has been shown to induce the expression of one or more ‘stress proteins’ in mammalian cells and tissues. A less severe form of oxygen depletion, hypoxic hypoxia, occurs in response to hypobaric decompression which simulates high altitude conditions. Under these conditions mouse hearts accumulate mRNAs for at least two polypeptides at substantially elevated levels. The molecular weights of these proteins, 85 kDa and 95 kDa, are similar to those reported for other mammalian stress proteins or glucose-regulated proteins. Time course experiments suggest that mRNAs for these species increase continuously for up to 16 hours of treatment, while mRNA for 71 kDa and 79 kDa polypeptides are elevated early in the treatment, but later decrease to control values. Total heart mRNA template activity is also increased by the hypobaric treatment. These results demonstrate that mouse cardiac tissue is capable of mounting a cellular stress-like response when exposed to moderately stressful conditions. It also provides a model for studying the direct effects of acute hypoxic stress on cellular gene expression, and its relationship to physiological adaptation.
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References
Schlesinger MJ, Ashburner M, Tissieres A: Heat Shock from Bacteria to Man. Cold Spring Harbor Laboratory, NY, 1982.
Welch WJ, Garrels JI, Feramisco JR: In: Heat Schock from Bacteria to Man, Schlesinger MJ, Ashburner M, Tissieres A (eds), Cold Spring Harbor Laboratory, NY, pp. 257–266, 1982.
Hammond GL, Lai YK, Markert CL: Proc Nat Acad Sci USA 79, 3485, 1982.
Currie RW, White FP: Science 214, 72, 1981.
Payvar F, Shimke RT: Eur J Biochem 101, 271, 1979.
Brawerman G, Mendecki J, Lee SY: Biochemistry 11, 637, 1972.
Pelham HRB, Jackson RJ: Eur J Biochem 67, 247, 1976.
Youden WJ: In: Statistical Methods for Chemists. John Wiley & Sons Inc., New York, pp. 40–49, 1951.
Eldridge SA, Hilton FK, Hilton MA: Fed Proc 42, 979, 1983.
Sciandra JJ, Subjeck JR: J Biol Chem 258, 12091, 1983.
Sciandra JJ, Subjeck JR, Hughes CS: Proc Nat Acad Sci USA 81, 4843, 1984.
Hightower LE, White FP: J Cell Physiol 108, 261, 1982.
Cosgrove JW, Brown IR, Proc Natl Acad Sci USA 80, 569, 1983.
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Howard, G., Geoghegan, T.E. Altered cardiac tissue gene expression during acute hypoxic exposure. Mol Cell Biochem 69, 155–160 (1986). https://doi.org/10.1007/BF00224762
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DOI: https://doi.org/10.1007/BF00224762