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Limited DNA elimination from the irradiated potato parent in fusion products of albino Lycopersicon esculentum and Solanum tuberosum

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Summary

This paper describes the analysis of the elimination of potato DNA from potato-tomato somatic cell hybrids. The hybrids were obtained by fusion of protoplasts of a cytoplasmic albino tomato genotype with leaf mesophyll protoplasts of a potato genotype carrying the β-glucuronidase (GUS) gene of Escherichia coli. The potato protoplasts were either isolated from unirradiated plants or from plants irradiated with 50 or 500 Gy of γ-rays. Green calli were selected as putative fusion products. The hybridity of these calli was confirmed by isoenzyme analysis. All of the green calli tested contained a potato-specific chloroplast DNA restriction fragment, and most of the calli analysed were positive for β-glucuronidase activity. In 72 of the hybrid calli we determined the percentage of potato nuclear DNA using species-specific probes. All of the tested green calli contained a considerable amount of potato genomic DNA, irrespective of the dose of irradiation of the potato parent. The limited degree of potato DNA elimination and the absence of true cybrids are discussed.

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References

  • Bates GW, Hasenkampf CA, Contolini CL, Piastuch WC (1987) Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts. Theor Appl Genet 74:718–726

    Google Scholar 

  • Bonierbale MW, Plaisted RL, Tanksley SD (1988) RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095–1103

    Google Scholar 

  • Bonnema AB, Melzer JM, O'Connell MA (1991) Tomato cybrids with mitochondrial DNA from Lycopersicon pennellii. Theor Appl Genet 81:339–348

    Google Scholar 

  • De Haas JM, Boot KJM, Haring MA, Kool AJ, Nijkamp HJJ (1986) A Petunia hybrida chloroplast DNA region, close to one of the inverted repeats, shows sequence homology with the Euglena gracilis chloroplast DNA region that carries the putative replication origin. Mol Gen Genet 202:48–54

    Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21

    CAS  Google Scholar 

  • Derks FHM, Hakkert JC, Colijn-Hooymans CM (1990) Organelle transfer from Solanaceous and Lycopersicon species to tomato via protoplast fusion. In: Cuartero J, Gomez-Guillamon ML, Fernandez-Munoz R (eds) Eucarpia tomato '90. Proceedings of the XIth meeting on tomato genetics and breeding. Torremolinos, Spain, March 6–8 1990, pp 179–184

  • Derks FHM, Wijbrandi J, Koornneef M, Colijn-Hooymans CM (1991) Organelle analysis of symmetric and asymmetric hybrids between Lycopersicon peruvianum and Lycopersicon esculentum. Theor Appl Genet 81:199–204

    Google Scholar 

  • De Vries SE, Ferwerda MA, Loonen AEHM, Pijnacker LP, Feenstra WJ (1987) Chromosomes in somatic hybrids between Nicotiana plumbaginifolia and a monoploid potato. Theor Appl Genet 75:170–176

    Google Scholar 

  • Dudits D, Fejér O, Hadlaczky G, Koncz C, Lázár GB, Horváth G (1980) Intergeneric gene transfer mediated by plant protoplast fusion. Mol Gen Genet 179:283–288

    Google Scholar 

  • Famelaer I, Gleba YY, Sidorov VA, Kaleda VA, Parokonny AS, Boryshuk NV, Cherep NN, Negrutiu I, Jacobs M (1989) Intrageneric asymmetric hybrids between Nicotiana plumbaginifolia and Nicotiana sylvestris obtained by ‘gamma-fusion’. Plant Sci 61:105–117

    Google Scholar 

  • Frearson EM, Power JB, Cocking EC (1973) The isolation, culture and regeneration of Petunia leaf protoplasts. Dev Biol 33:130–137

    Google Scholar 

  • Gupta PP, Schieder O, Gupta M (1984) Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion. Mol Gen Genet 197:30–35

    Google Scholar 

  • Herrmann RG, Westhoff P, Alt J, Tittgen J, Nelson N (1985) Thylakoid membrane proteins and their genes. In: van Vloten-Doting L, Groot GSP, Hall TC (eds) Molecular form and function of the plant genome. Plenum Publ, New York, pp 233–256

    Google Scholar 

  • Hosticka LP, Hanson MR (1984) Induction of plastid mutations in tomatoes by nitrosomethylurea. J Hered 75:242–246

    Google Scholar 

  • Imamura J, Saul MW, Potrykus I (1987) X-ray irradiation promoted asymmetric somatic hybridisation and molecular analysis of the products. Theor Appl Genet 74:445–450

    Google Scholar 

  • Jain SM, Shahin EA, Sun S (1988) Interspecific protoplast fusion for the transfer of atrazine resistance from Solanum nigrum to tomato (Lycopersicon esculentum L.) Plant Cell Tissue Organ Cult 12:189–192

    Google Scholar 

  • Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405

    CAS  Google Scholar 

  • Kirk JTO, Tilney-Bassett RAE (1978) The plastids. Their chemistry, structure, growth and inheritance. Elsevier/North-Holland Biomedical Press, Amsterdam New York Oxford, pp 460–486

    Google Scholar 

  • Kushnir S, Babiychuk E, Bannikova M, Momot V, Komarnitsky I, Cherep N, Gleba Y (1991) Nucleo-cytoplasmic incompatibility in cybrid plants possessing an Atropa genome and a Nicotiana plastome. Mol Gen Genet 225:225–230

    Google Scholar 

  • Medgyesy P (1990) Selection and analysis of cytoplasmic hybrids. In: Dix PJ (ed) Plant cell line selection. Procedures and applications. VCH, Weinheim New York Basel Cambridge, pp 287–316

    Google Scholar 

  • Melchers G, Sacristán MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Res Commun 43:203–218

    Google Scholar 

  • Menczel L, Galiba G, Nagy F, Maliga P (1982) Effect of radiation dosage on efficiency of chloroplast transfer by protoplast fusion in Nicotiana. Genetics 100:487–495

    Google Scholar 

  • Mettler IJ (1987) A simple and rapid method for minipreparation of DNA from tissue cultured plant cells. Plant Mol Biol Rep 5:346–349

    Google Scholar 

  • Müller-Gensert E, Schieder O (1987) Interspecific T-DNA transfer through plant protoplast fusion. Mol Gen Genet 208:235–241

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Parthier B (1982) The cooperation of nuclear and plastid genomes in plastid biogenesis and differentiation. Biochem Physiol Pflanz 177:283–317

    Google Scholar 

  • Perl A, Aviv D, Galun E (1990) Protoplast-fusion-derived Solanum cybrids: application and phylogenetic limitations. Theor Appl Genet 79:632–640

    Google Scholar 

  • Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissues. Plant molecular biology manual A6. Kluwer Academic Publ, Dordrecht, pp 1–10

    Google Scholar 

  • Saul MW, Potrykus I (1984) Species-specific repetitive DNA used to identify interspecific somatic hybrids. Plant Cell Rep 3:65–67

    Google Scholar 

  • Schiller B, Herrmann RG, Melchers G (1982) Restriction endonuclease analysis of plastid DNA from tomato, potato and some of their somatic hybrids. Mol Gen Genet 186:453–459

    Google Scholar 

  • Shahin EA (1985) Totipotency of tomato protoplasts. Theor Appl Genet 69:235–240

    Google Scholar 

  • Shepard JF, Bidney D, Barsby T, Kemble R (1983) Genetic transfer in plants through interspecific protoplast fusion. Science 219:683–688

    Google Scholar 

  • Tewes A, Glund K, Walther R, Reinbothe H (1984) High yield isolation and rapid recovery of protoplasts from suspension cultures of tomato (Lycopersicon esculentum). Z Pflanzenphysiol 113:141–150

    Google Scholar 

  • Thanh ND, Medgyesy P (1989) Limited chloroplast gene transfer via recombination overcomes plastome-genome incompatibility between Nicotiana tabacum and Solanum tuberosum. Plant Mol Biol 12:87–93

    Google Scholar 

  • Uchimiya H, Murashige T (1974) Evaluation of parameters in the isolation of viable protoplasts from cultured tobacco cells. Plant Physiol 54:936–944

    Google Scholar 

  • Vallejos CE (1983) Enzyme activity staining. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier Science Publ, Amsterdam, pp 469–516

    Google Scholar 

  • Visser RGF, Hoekstra R, Van der Leij FR, Pijnacker LP, Witholt B, Feenstra WJ (1988) In situ hybridization to somatic metaphase chromosomes of potato. Theor Appl Genet 76:420–424

    Google Scholar 

  • Wijbrandi J, Zabel P, Koornneef M (1990) Restriction fragment length polymorphism analysis of somatic hybrids between Lycopersicon esculentum and irradiated L. peruvianum: Evidence for limited donor genome elimination and extensive chromosome rearrangements. Mol Gen Genet 222:270–277

    Google Scholar 

  • Yamashita Y, Terada R, Nishibayashi S, Shimamoto K (1989) Asymmetric somatic hybrids of Brassica: partial transfer of B. campestris genome into B. oleracea by cell fusion. Theor Appl Genet 77:189–194

    Google Scholar 

  • Zabel P, Meyer D, Van de Stolpe O, Van der Zaal B, Ramanna MS, Koornneef M, Krens F, Hille J (1985) Towards the contstruction of artificial chromosomes for tomato. In: van Vloten-Doting L, Groot GSP, Hall TC (eds) Molecular form and function of the plant genome. Plenum Publ, New York, pp 609–624

    Google Scholar 

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Authors and Affiliations

  1. Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands

    A. M. A. Wolters, H. C. H. Schoenmakers, J. J. M. van der Meulen-Muisers, E. van der Knaap & M. Koornneef

  2. Centre for Plant Breeding and Reproduction Research CPRO, P.O. Box 16, NL-6700 AA, Wageningen, The Netherlands

    E. H. M. Derks

  3. Division of Genetics and Plant Breeding, Institute of Field and Garden Crops, Volcani Centre, Agricultural Research Organization, 50250, Bet-Dagan, Israel

    A. Zelcer

Authors
  1. A. M. A. Wolters
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  2. H. C. H. Schoenmakers
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  3. J. J. M. van der Meulen-Muisers
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  4. E. van der Knaap
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  5. E. H. M. Derks
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  6. M. Koornneef
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  7. A. Zelcer
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Communicated by I. Potrykus

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Wolters, A.M.A., Schoenmakers, H.C.H., van der Meulen-Muisers, J.J.M. et al. Limited DNA elimination from the irradiated potato parent in fusion products of albino Lycopersicon esculentum and Solanum tuberosum . Theoret. Appl. Genetics 83, 225–232 (1991). https://doi.org/10.1007/BF00226255

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  • DOI: https://doi.org/10.1007/BF00226255

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