Summary
Chromosome constitutions of three wheat-Agropyron intermedium derivatives were identified by C-banding analysis, in situ hybridization using biotin-labeled genomic Ag. intermedium DNA as a probe and isozyme analysis. Lines W44 and W52 were identified as 7Ai-2(7D) and 7Ai-2(7A) chromosome substitution lines carrying the same chromosome pair of Ag. intermedium. The alien chromosome was found to be homoeologous to group 7 based on C-banding, meiotic pairing and isozyme analyses. Line W49 was identified as a wheat Ag. intermedium chromosome translocation line. The breakpoint of the T2AS · 2AL-7Ai-2L translocation is located in the long arm at a fraction length of 0.62, and the transferred Ag. intermedium segment has a size of about 2.4 μm. Lines W44 and W52 expressed Ag. intermedium genes for resistance to leaf rust, stripe rust and stem rust, but only leaf rust resistance was expressed in W49. The results show that the leaf rust resistance gene(s), designated Lr38, is located in the distal half of the long arm of chromosome 7Ai-2, whereas the genes for resistance to stem rust and stripe rust are located either in the short arm or in the proximal region of the long arm of this chromosome.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anamthawat-Jonsson K, Schwarzacher T, Leitsch AR, Heslop-Harrison JS (1990) Discrimination between closely related Triticeae species using genomic DNA as a probe. Theor Appl Genet 79:721–728
Cauderon Y (1966) Étude cytogénétique de l'évolution du matériel issu de croisement entre Triticum aestivum et Agropyron intermedium. Ann Amelior Plant 16:43–70
Cauderon Y, Rhind J-M (1976) The effect on wheat of an Agropyron chromosome carrying stripe rust resistance. Ann Amelior Plant 26:745–749
Cauderon Y, Saigne B, Dauge M (1973) The resistance to wheat rusts of Agropyron intermedium and its use in wheat improvement. In: Sears ER, Sears LMS (eds) Proc 4th Int Wheat Genet Symp. University of Missouri, Columbia, Mo., pp 401–407
Dewey DR (1984) The genomic system of classification as a guide to intergeneric hybridization within perennial Triticeae. In: Gustafson JP (ed) Gene manipulation in plant improvement. Plenum Publ, New York, pp 209–279
Dvořak J (1981a) Genome relationships among Elytrigia (=Agropyron) elongata, E. stipifolia, “E. elongata 4 x”, E. caespitosa, E. intermedia, and “E. elongata 10 x”. Can J Genet Cytol 23:481–492
Dvořak J (1981b) Chromosome differentiation in polyploid species of Elytrigia, with special reference to the evolution of diploid-like chromosome pairing in polyploid species. Can J Genet Cytol 23:287–303
Endo TR, Gill BS (1984) The heterochromatin distribution and genome evolution in diploid species of Elymus and Agropyron. Can J Genet Cytol 26:669–678
Forster BP, Reader SM, Forsyth SA, Koebner RMD, Miller TE, Gale MD, Cauderon Y (1987) An assessment of the homoeology of six Agropyron intermedium chromosomes added to wheat. Genet Res 50:91–97
Friebe B, Mukai Y, Dhaliwal HS, Martin TJ, Gill BS (1991a) Identification of alien chromatin specifying resistance to wheat streak mosaic virus and greenbug in wheat germplasm by C-banding and in situ hybridization. Theor Appl Genet 81:381–389
Friebe B, Hatchett JR, Gill BS, Mukai Y, Sebesta EE (1991b) Transfer of Hessian fly resistance from rye to wheat via radiationinduced terminal and intercalary chromosomal translocations. Theor Appl Genet 83:33–40
Gill BS, Friebe B, Endo TR (1991) Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum L.). Genome 34:830–839
Hsam SLK, Zeller FJ (1982) Relationships of Agropyron intermedium chromosomes determined by chromosome pairing and alcohol dehydrogenase isozymes in common wheat background. Theor Appl Genet 63:213–217
Knott DR (1961) The inheritance of rust resistance VI. The transfer of stem rust resistance from Agropyron elongatum to common wheat. Can J Plant Sci 41:109–123
Knott DR (1989) The wheat rusts —breeding for resistance. Springer, Berlin Heidelberg New York
Koebner RMD, Miller TE, Snape JW, Law CM (1988) Wheat endopeptidase: genetic control, polymorphism, intrachromosomal location and alien variation. Genome30:186–192
Lapitan NLV, Sears RG, Rayburn AL, Gill BS (1986) Detection of chromosome breakpoints by in situ hybridization with a biotin-labeled DNA probe. J Hered 77:415–419
Le HT, Armstrong KC, Miki B (1989) Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe. Plant Mol Biol Rep 7:150–158
McIntosh RA (1988) Catalogue of gene symbols for wheat. In: Miller TE, Koebner RMD (eds), 7th Int Wheat Genet Symp. Cambridge UK, pp 1225–1323
Mukai Y, Gill BS (1991) Detection of barley chromatin added to wheat by genomic in situ hybridization. Genome 34:448–452
Mukai Y, Friebe B, Hatchett JH, Gill BS (1992) Detection of rye chromatin in wheat specifying resistance to Hessian fly by in situ hybridization using total rye genomic DNA probes. In: Proc 2nd Int Symp Chromosome Engineering in Plants. Columbia Mo. (in press)
Rayburn AL, Gill BS (1985) Use of biotin-labeled probes to map specific DNA sequences on wheat chromosomes. J Hered 76:78–81
Sears ER (1956) The transfer of leaf-rust resistance from Aegilops umbellulata to wheat. Brookhaven Symp Biol 9:1–21
Sears ER (1973) Agropyron-wheat transfers induced by homoeologous pairing. In: Sears ER, Sears LMS (eds) Proc 4th Int Wheat Genet Symp. University of Missouri, Columbia, Mo., pp 191–199
Sharma D, Knott DR (1966) The transfer of leaf-rust resistance from Agropyron to Triticum by irradiation. Can J Genet Cytol 8:137–143
Sinigovets ME (1976) Transfer of rust resistance from Agropyron to wheat by chromosome addition and substitution. Genetika 12:13–21
The TT, Baker EP (1970) Homoeologous relationships between two Agropyron intermedium chromosomes and wheat. Wheat Inf Serv 31:29–31
Wienhues A (1960) Die Ertragsleistung rostresistenter 44-und 42chromosomiger Weizen-Quecken-Bastarde. Der Züchter 30:194–202
Wienhues A (1966) Transfer of rust resistance of Agropyron to wheat by addition substitution and translocation. In: Proc 2nd Int Wheat Genet Symp. Hereditas [Suppl] 2:328–341
Wienhues A (1967) Die Übertragung der Rostresistenz aus Agropyron intermedium in den Weizen durch Translokation. Der Züchter 37:345–352
Wienhues A (1971) Substitution von Weizenchromosomen aus verschiedenen homoeologen Gruppen durch ein Fremdchromosom aus Agropyrum intermedium. Z Pflanzenzucht 65:307–321
Wienhues A (1973) Translocations between wheat chromosomes and an Agropyron chromosome conditioning rust resistance. In: Sears ER, Sears LMS (eds) Proc 4th Int Wheat Genet Symp. University of Missouri, Columbia Mo., pp 201–207
Wienhues A (1979) Translokationslinien mit Resistenz gegen Braunrost (Puccinia recondita) aus Agropyrum intermedium. Ergebnisse aus der Rückkreuzung mit Winterweizensorten. Z Pflanzenzücht 82:149–161
Author information
Authors and Affiliations
Additional information
Communicated by G. S. Khush
Rights and permissions
About this article
Cite this article
Friebe, B., Zeller, F.J., Mukai, Y. et al. Characterization of rust-resistant wheat-Agropyron intermedium derivatives by C-banding, in situ hybridization and isozyme analysis. Theoret. Appl. Genetics 83, 775–782 (1992). https://doi.org/10.1007/BF00226697
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00226697