Summary
Grain isozyme and ribosomal DNA (rDNA) variability was examined in Hordeum spontaneum populations sampled from 27 geographical sites in Israel. Considerable phenotypic variability was observed with variants of ADH1, EST3, EST10, BMY1 and WSP detected, which are not available in the H. vulgare gene pool. Seven new rDNA phenotypes were detected in the H. spontaneum populations. Shannon's index of diversity was used to partition the total phenotypic variation into between and within population components. Most of the variation occurred between H. spontaneum populations. The distribution of both grain isozyme and rDNA phenotypes was non-random and correlated with a range of ecogeographical factors. In particular, the G phenotype of BMY1 was restricted to the Negev Desert and Dead Sea regions of Israel. Over 78% of the variation in the frequency of this particular phenotype could be explained by the number of rainy days per year and mean temperature in January. This suggests that variation at this locus or at loci linked to it may be of adaptive significance and of value in the introgression of genes controlling abiotic stress tolerance from H. spontaneum into the H. vulgare gene pool.
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References
Allard RW, Saghai-Maroof MA, Zhang Oifa, Jorgensen RA (1990) Genetic and molecular organisation of ribosomal DNA (rDNA) variants in wild and cultivated barley. Genetics 126:743–751
Cluster PD, Marinkovic R, Allard RW, Ayala FJ (1987) Corre-l ations between development rates, enzyme activities, ribosomal DNA spacer-length phenotypes and adaption in Drosophila melanogaster. Proc Natl Acad Sci USA 84:610–614
Feinberg AP, Vogelstein B (1984) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137:266–267
Flavell RB, O'Dell M, Sharp P, Nevo E (1986) Variation in the intergenic spacer of ribosomal DNA of wild wheat, Triticum dicoccoides, in Israel. Mol Biol Evol 3:547–558
Forster BP, Ellis RP (1991) Two biochemical markers for spring/winter habit in barley. In: Barley genetics VI. Sweden, pp 65–67
Forster BP, Thompson DM, Watters J, Powell W (1991) Water-soluble proteins of mature barley endosperm: genetic control, polymorphism and linkage with β-amylase and spring/ winter habit. Theor Appl Genet 81:787–792
Frankel OH, Bennet E (1970) Genetic resources in plants — their exploration and conservation. Blackwell, Oxford
Goldsborough PB, Cullis CA (1981) Characterisation of the genes for ribosomal RNA in flax. Nucleic Acids Res 9:1301–1309
Hackett CA, Ellis RP, Forster BP, McNicol JW, Macaulay M (1992) Statistical analysis of a linkage experiment in barley involving quantitative trait loci for height and ear emergence time and two genetic markers on chromosome 4. Theor Appl Genet (in press)
Harlan JR (1979) Barley. In: Simmonds NW (ed) Evolution of crop plants. Longam, London, pp 93–98
Hvid S, Nielsen G (1977) Esterase isoenzyme variants in barley. Hereditas 87:155–162
King LM, Schaal BA (1989) Ribosomal-DNA variation and distribution in Rudbeckia missouriensis. Evolution 43:1117–1119
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Nevo E (1986) Genetic resources of wild cereals and crop improvement: Israel, a natural laboratory. Isr J Bot 35:255–278
Nevo E (1991) Origin, evolution, population genetics and resources for breeding of wild barley, Hordeum spontaneum, in the fertile crescent. In: Shewry P (ed) Genetics, biochemistry, molecular biology and Biotechnology. CAB Int, UK
Nevo E, Zohary D, Brown AHD, Haber M (1979) Genetic diversity and environmental associations of wild barley, Hordeum spontaneum, in Israel. Evolution 33:815–833
Nevo E, Brown AHD, Zohary D, Storch N, Beiles A (1981) Microgeographic edaphic differentiation, in allozyme polymorphisms of wild barley, Hordeum spontaneum, Poaceae. Plant Syst Evol 91: 138:287–292
Nevo E, Golenberg EM, Beiles A, Brown AHD, Zohary D (1982) Genetic diversity and environmental associations of wild wheat, Triticum dicoccoides in Israel. Theor Appl Genet 62:241–254
Nevo E, Beiles A, Stork N, Doll H, Anderson B (1983) Microgeographic edaphic differentiation in hordein polymorphisms of wild barley. Theor Appl Genet 64:122–132
Nevo E, Beiles A, Zohary D (1986) Genetic resources of wild barley in the Near East: structure, evolution and application in breeding. J Linn Soc London Bot: 27:355–380
Nielsen G, Hejgaard J (1986) Mapping of isozyme and protein loci in barley. Isozymes Curr Top Biol Med Res 15:77–95
Powell W, Ellis RP, Macaulay M, McNicol J, Forster BP (1990) The effect of selection for protein and isozyme loci on quantitative traits in a doubled haploid population of barley. Heredity 65:115–122
Powell W, Thomas WTB, Thompson DM, Swanston JS, Waugh R (1991) Associations between rDNA alleles and quantitative traits in doubled haploid populations of barley. Genetics 130:187–194
Reed KC, Mann DA (1985) Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res 13:7207–7221
Rocheford TR, Osterman JC, Gardner CO (1990) Variation in the ribosomal DNA intergenic spacer of a maize population mass-selected for high grain yield. Theor Appl Genet 79:793–800
Saghai-Maroof MS, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Saghai-Maroof MA, Allard RW, Zhang Q (1990) Genetic diversity and ecogeographical differentiation among ribosomal DNA alleles in wild and cultivated barley. Proc Natl Acad Sci US A 87:8486–8490
Thompson DM, Powell W, Forster BP (1990) Use of isoelectric focussing in barley varietal identification. Ann Appl Biol 117:625–631
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Chalmers, K.J., Waugh, R., Watters, J. et al. Grain isozyme and ribosomal DNA variability in Hordeum spontaneum populations from Israel. Theoret. Appl. Genetics 84, 313–322 (1992). https://doi.org/10.1007/BF00229489
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DOI: https://doi.org/10.1007/BF00229489