Summary
A cytosolic protein, able to facilitate intermembrane movements of phospholipids in vitro, has been purified to homogeneity from sunflower seedlings. This protein, which has the properties of a lipid-transfer protein (UP), is also able to bind oleoyl-CoA, as shown by FPLC chromatography. This finding, in addition to previous observations suggesting that a lipid-transfer protein from spinach leaves can bind oleic acid and that oat seedlings contain a fatty acid-binding protein with similar features than lipid transfer proteins, provides a clear demonstration that plant cells contain bifunctional fatty acid/lipid transfer proteins. These proteins can play an active role in fatty acid metabolism which involves movements of oleyl-CoA between intracellular membranes.
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Abbreviations
- FABP:
-
Fatty Acid-Binding Proteins
- UP:
-
Lipid-Transfer Protein
- PC:
-
Phosphatidylcholine
- PI:
-
Phosphatidylinositol
- PE:
-
Phosphatidylethanolamine
- pI:
-
Isoelectric point
References
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Arondel, V., Vergnolle, C., Tchang, F. et al. Bifunctional lipid-transfer: fatty acid-binding proteins in plants. Mol Cell Biochem 98, 49–56 (1990). https://doi.org/10.1007/BF00231367
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DOI: https://doi.org/10.1007/BF00231367