Abstract
Somatic embryogenesis was induced in hypocotyls of Digitalis obscura using indoleacetic acid or 2,4-dichlorophenoxyacetic acid with different culture and subculture conditions. Indoleacetic acid-induced embryogenic cultures were used to investigate the effects of amino acids, polyamines and growth regulators on embryo differentiation and maturation. Supplementation of the media with amino acids, polyamines or abscisic acid did not influence or had an adverse effect on embryogenic response. Gibberellic acid at 1.4 μM in either culture (30 days) or subculture medium was effective in promoting both differentiation and normal embryo development. The efficiency of somatic embryogenesis was greatly enhanced when isolated indoleacetic acid-induced proembryogenic masses were subcultured in liquid medium with reduced auxin content.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ABA:
-
abscisic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- GA:
-
gibberellic acid
- IAA:
-
indoleacetic acid
- Ptr:
-
putrescine
- Spd:
-
spermidine
- Spn:
-
spermine
References
Altman A, Nadel BL, Falash, Levin N (1990) In: Nijkamp Van der Plas LHW, Aartrijk J van (eds) Progress in Plant Cellular and Molecular Biology. Kluwer Academic Publishers, Dordrecht, pp 454–459
Ammirato PV (1974) Bot Gaz 135:328–337
Ammirato PV (1983) Biotech 1:68–74
Arnold S von, Hakman I (1988) J Plant Physiol 132:164–169
Arrillaga I, Brisa MC, Segura J (1986) Plant Physiol 124:425–430
Arrillaga I, Brisa MC, Segura J (1987) Plant Cell Rep 6:223–226
Bonner C, Rodrigues A, Miller J, Jensen R (1992) Physiol Plant 84:319–328
Eapen S, George L (1990) Plant Cell Tissue Organ Culture 22:87–93
Fujimura T, Komamine A (1975) Plant Sci Lett 5:359–364
Gavidia I, Segura J, Pérez-Bermúdez P (1993) J Plant Physiol 142:373–376
Kamada H, Harada H (1981) Plant Cell Physiol 22:1423–1429
Komamine A, Matsumoto M, Tsukajara M, Fujiwara A, Kawajara R, Ito M, Smith J, Nomura K, Fujimura T (1990) In: Nijkamp HJJ, Van der Plas LHW, Aartrijk J van (eds) Progress in Plant Cellular and Molecular Biology. Kluwer Academic Publishers, Dordrecht, pp 307–313.
Komatsuda T, Wenbin L, Seibi O (1992) Plant Cell Tissue Organ Culture 28:103–113
Lehminger-Mertens R, Jacobsen H-J (1989) Plant Cell Rep 8:379–382
Leshem B, Gilboa N, Izhar S, Frankel R (1989) Plant Physiol 134:573–576
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Nadel BL, Altaian A, Ziv M (1990) Plant Cell Tissue Organ Culture 20:119–124
Pérez-Bermúdez P, Cornejo MJ, Segura J (1983) Plant Sci Lett 30:77–82
Robie CA, Minocha SC (1989) Plant Sci 65:45–54
Strickland SG, Nichol JW, McCall ZM, Stuart DA (1987) Plant Sci 48:113–121
Tisserat B, Murashige T (1977) In Vitro 13:799–805
Verma DC, Tarka T (1985) In: Terzi M, Pitto L, Sung ZR (eds) Somatic Embryogenesis. IPRA, Rome, pp 151
Author information
Authors and Affiliations
Additional information
Communicated by A.M. Boudet
Rights and permissions
About this article
Cite this article
Lapeña, L., Brisa, M.C. Influence of culture conditions on embryo formation and maturation in auxin-induced embryogenic cultures of Digitalis obscura . Plant Cell Reports 14, 310–313 (1995). https://doi.org/10.1007/BF00232034
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232034