Abstract
A procedure for rapid in vitro propagation of the aromatic and medicinal plant Hemidesmus indicus (L.) R.Br. (Family Asclepiadaceae) from nodal explants is described. The highest shoot multiplication rate of 8.2 ± 0.4 shoots/explant with a 95% frequency was achieved in S weeks culture period on Murashige and Skoog medium supplemented with 1.15 μM kinetin and 0.054 μM α-naphthaleneacetic acid. Excised shoots were rooted on the same basal medium supplemented with 1.15 μM kinetin and 7.35 μM indole-3-butyric acid. Shoots derived from subcultures exhibited better rooting response than those from primary cultures. After a hardening phase of 2 weeks, there was a 70% transplantation success in the field.
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Abbreviations
- MS:
-
Murashige and Skoog (1962) medium
- BA:
-
N6 benzyladenine; KN kinetin
- NAA:
-
a-naphthaleneacetic acid
- IBA:
-
indole-3-butyric acid
- IAA:
-
indole-3-acetic acid
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Communicated by G. C. Phillips
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Patnaik, J., Debata, B.K. Micropropagation of Hemidesmus indicus (L.) R. Br. through axillary bud culture. Plant Cell Reports 15, 427–430 (1996). https://doi.org/10.1007/BF00232069
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DOI: https://doi.org/10.1007/BF00232069