Abstract
Several culture conditions were examined for promoting efficient plant regeneration from explants of Gentiana. Adventitious shoot regeneration from leaf explants of cv. WSP-3 was very superior on MS medium, compared to B5 medium, supplemented with four cytokinins (TDZ, 4PU-30, BA and zeatin). An auxin / cytokinin combination was required for regeneration. TDZ was the most effective cytokinin, while NAA was more effective than IAA or 2,4-D. Optimum conditions for regeneration from explants (leaf, stem and root) of cv. WSP-3, evaluated in terms of regeneration frequency and number of regenerated shoots per explant, were TDZ and NAA in combination, 5–10 mg/l and 0.1 mg/l for leaf and stem explants, and 10 mg/l and 1 mg/l for root explants, respectively. Application of these conditions to eight other commercial cultivars resulted in 30–100% regeneration from leaf explants. The number of chromosomes in each of ten regenerated plants of each cultivar was diploid, 2n=26. Shoots regenerated in vitro were rooted in phytohormone-free medium and transferred to soil.
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Abbreviations
- MS medium:
-
Murashige and Skoog's medium (Murashige and Skoog 1962)
- B5 medium:
-
Gamborg B5 medium (Gamborg et al. 1968)
- BA:
-
6-benzylaminopurine
- TDZ:
-
N-phenyl-N'-1,2,3-thiadiazol-5-yl urea
- 4PU-30:
-
N-(2-chloro-4-pyridyl)-N'-phenylurea
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indole-3-acetic acid
- NAA:
-
1-naphthaleneacetic acid
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Communicated by I. K. Vasil
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Hosokawa, K., Nakano, M., Oikawa, Y. et al. Adventitious shoot regeneration from leaf, stem and root explants of commercial cultivars of Gentiana . Plant Cell Reports 15, 578–581 (1996). https://doi.org/10.1007/BF00232456
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DOI: https://doi.org/10.1007/BF00232456