Abstract
The subcellular localization of tryptophan decarboxylase, strictosidine synthase and strictosidine glucosidase in suspension cultured cells of Catharanthus roseus (L.) G. Don and Tabernaemontana divaricata (L.) R. Br. ex Roem. et Schult, was investigated. It was found that tryptophan decarboxylase is an extra-vacuolar enzyme, whereas strictosidine synthase is active inside the vacuole. Strong indications were obtained for the localization of strictosidine glucosidase on the outside of the tonoplast. The results suggest that tryptamine is transported into the vacuole where it is condensed with secologanin to form strictosidine, and that strictosidine passes the tonoplast and is subsequently hydrolysed outside the vacuole.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- AM:
-
α-mannosidase
- EDTA:
-
ethylenediaminetetraacetic acid
- Hepes:
-
N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
- HPLC:
-
highperformance liquid chromatography
- MDH:
-
malate dehydrogenase
- SG:
-
strictosidine glucosidase
- SSS:
-
strictosidine synthase
- TDC:
-
tryptophan decarboxylase
References
Bergmeyer HU, Bernt E (1965) Methoden der enzymatische Analyse. Verlag Chemie, Weinheim
Betz M, Martinoia E, Hincha DK, Schmitt JM, Dietz K-J (1992) Phytochemistry 31: 433–440
Blom TJM, Sierra M, Van Vliet TB, Franke-van Dijk MEI, De Koning P, Van Iren F, Verpoorte R, Libbenga KR (1991) Planta 183: 170–177
Blom TJM 1991. — PhD Thesis, Leiden University
Boller T, Kende H (1979) Plant Physiol. 63: 1123–1132
DeLuca V, Cutler AJ (1987) Plant Physiol. 85: 1099–1102
Deus-Neumann B, Zenk MH (1984) Planta 162: 250–260
Doireau P, Mérillon JM, Guillot A, Rideau M, Chénieux JC, Brillard M (1987) Planta Med. 53: 364–367
Grünweller S, Kesselmeier J (1985) Phytochemistry 24: 1941–1943
Hampp N, Zenk MH (1988) Phytochemistry 27: 3811–3815
Kutney JP, Choi LSL, Kolodziejczyk P, Sleigh SK, Stuart KL, Worth BR, Kurz WGW, Chatson K.B, Constabel F (1981) J. Nat. Prod. 44: 536–540
Linsmaier EM, Skoog F (1965) Physiol. Plant. 18: 100–127
Masuda H, Narita S, Sugawara S (1990) Phytochemistry 29: 393–397
McKnight TD, Bergey DR, Burnett RJ, Nessler CL (1991). Planta 185: 148–152
Noé W, Mollenschott C, Berlin J (1984) Plant Mol. Biol. 3: 281–288
Pasquali G, Goddijn OJM, Waal A de, Verpoorte R, Schilperoort RA, Hoge JHC, Memelink J (1992) Plant Mol. Biol. 18: 1121–1131
Pennings EJM, Hegger I, Van der Heijden R, Duine JA, Verpoorte R (1987) Anal. Biochem. 165: 133–136
Pennings EJM, Van den Bosch RA, Van der Heijden R, Stevens LH, Duine JA, Verpoorte R (1989a) Anal. Biochem. 176: 412–415
Pennings EJM, Verpoorte R, Goddijn OJM, Hoge JHC (1989b) J. Chromatogr. 483: 311–318
Pfitzner U, Zenk MH (1989) Planta Med. 55: 525–530
Renaudin JP (1981) Plant Sci. Lett. 22: 59–69
Roewer IA, Cloutier N, Nessler CL, DeLuca V (1992) Plant Cell Rep. 11: 86–89
Schripsema J, Erkelens C, Verpoorte R (1991) Plant Cell Rep 9: 527–530
Skinner SE, Walton NJ, Robins RJ, Rhodes MJC (1987) Phytochemistry 26: 721–725
Stevens LH, Schripsema J, Pennings EJM, Verpoorte R (1992) Plant Physiol. Biochem. 30: 675–681
Stevens LH, Giroud C, Pennings EJM, Verpoorte R (1993) Phytochemistry, in press
Stöckigt J, Treimer J, Zenk MH (1976) FEBS Letters 70: 267–270
Author information
Authors and Affiliations
Additional information
Communicated by W. Barz
Rights and permissions
About this article
Cite this article
Stevens, L.H., Blom, T.J.M. & Verpoorte, R. Subcellular localization of tryptophan decarboxylase, strictosidine synthase and strictosidine glucosidase in suspension cultured cells of Catharanthus roseus and Tabernaemontana divaricata . Plant Cell Reports 12, 573–576 (1993). https://doi.org/10.1007/BF00233063
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00233063