Abstract
Multiple shoots were induced on Valenciatype peanut (Arachis hypogaea L.) explants cultured in vitro on a nutrient medium supplemented with thidiazuron. Zygotic embryos excised from mature seeds were germinated on Murashige-Skoog nutrient medium, and the resulting plantlets (8 days-old) were used as a source of explants. When cultured on a nutrient medium with increasing levels of thidiazuron (0.5 to 30 mg/l), expiants from various parts of the peanut plant (except the root) produced multiple shoot primordia which subsequently developed into individual shoots. Hypocotyl and cotyledon explants produced shoots in higher numbers than other explants (20 shoots per hypocotyl explant at all thidiazuron concentrations and 15 shoots per cotyledon explant at 30 mg/l). Shoots rooted normally on a basal Murashige-Skoog medium containing charcoal and developed into healthy and fertile plants when planted in soil.
Similar content being viewed by others
Abbreviations
- TDZ:
-
thidiazuron
- MSO:
-
Murashige and Skoog (1962) basal medium
- BA:
-
6-benzylaminopurine
References
Baker CM, Wetzstein HY (1992) Plant Cell Reports 11: 71–75
Cheng M, Hsi CH, Phillips GC (1992) Peanut Science 19: 82–87
Clemente TE, Robertson D, Isleib TG, Beute MK, Weissinger AK (1992) Transgenic Research 1: 275–284
Cobb WY, Johnson BR (1973) (ed) Peanuts: Culture and Uses. American Peanut Res and Education Soc, Stillwater, OK
Durham, RE, Parrott WA (1992) Plant Cell Reports 11: 122–125
Franklin CI, Shorrosh KM, Cassidy BG, Nelson RS (1992) In Vitro Cellular and Devel Biol 28A: 121A
Gill R, Saxena PK (1992) Can J Bot 70: 1186–1192
Gill R, Saxena PK (1993) Plant Cell Reports 12: 154–159
La Corte C, Mansu E, Timmermann B, Cordeiro AR (1991) Plant Cell Reports 10: 354–357
McKently AH (1991) In Vitro Cell Dev Biol 27P: 197–200
McKently AH, Moore GA, Gardner FP (1990) Crop Sci 30: 192–196
McKently AH, Moore GA, Gardner FP (1991) Crop Sci 31: 833–837
McKently AH, Moore GA, Wang A, Doostdar H (1993) In Vitro Cell Dev Biol 29A: 65A
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Narasimhulu SB, Reddy GM (1983) Plant Science Letters 31: 157–163
Norden AT, Smith OD, Gorbet DW (1982) In: H. E. Pattee and C. T. Young (ed) Peanut Science and Technology. American Peanut Res and Education Soc, Yoakum, TX. pp 95–122
Ozias-Akins P, Anderson WF, Schnall JA, Singsit JA, Clemente TE, Weissinger, AK (1992) Proc of American Peanut Res and Edu Soc. 24: 19
Ozias-Akins P, Schnall JA, Anderson WF, Singsit C, Clemente TE, Adang, MJ, Weissinger, AK (1993) Plant Science 93: 185–194
Pattee HE, Johns EB, Singleton JA, Sanders TH (1974) Peanut Science 10: 57–62
Saxena PK, Malik KA, Gill R (1992) Planta 187: 421–424
Schnall JA, Weissinger AK (1993) Plant Cell Reports 12: 316–319
Sellars RM, Southward GM, Phillips GC (1990) Crop Sci 30: 408–414
Author information
Authors and Affiliations
Additional information
Communicated by I. K. Vasil
Rights and permissions
About this article
Cite this article
Kanyand, M., Dessai, A.P. & Prakash, C.S. Thidiazuron promotes high frequency regeneration of peanut (Arachis hypogaea) plants in vitro . Plant Cell Reports 14, 1–5 (1994). https://doi.org/10.1007/BF00233288
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00233288