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Distribution of LHRH in the rat and mouse brain with special reference to the tanycytes

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Summary

The distribution of luteinizing hormone-releasing hormone (LHRH) was studied in the rat and mouse brain by means of light and electron microscopic immunohistochemistry using the peroxidase-antiperoxidase method. An immunoreactive product to LHRH antiserum was found near the blood vessels of the vascular organ of the lamina terminalis. In the arcuate nucleus-median eminence region, an immunoreactive material occurred bilaterally in the hypothalamic tissue around the tuberoinfundibular sulci. Electron microscopy revealed that immunoreactive fibers observed light microscopically contain numerous granules 100–130 nm in diameter. No immunoreactive product was located in the tanycytes of the median eminence, the perikarya of hypothalamic neurons, and the parenchyma of several circumventricular organs (subfornical organ, subcommissural organ, pineal organ, area postrema).

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Author notes

  1. Masumi Nozaki &  Hideshi Kobayashi

    Present address: Department of Biology, Faculty of Science, Toho University, Funabashi, Japan

Authors and Affiliations

  1. Misaki Marine Biological Station, University of Tokyo, Miura-shi, Kanagawa-ken, Japan

    Masumi Nozaki, Yuji Taketani, Hiroshi Minaguchi, Tomonori Kigawa &  Hideshi Kobayashi

  2. Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan

    Masumi Nozaki, Yuji Taketani, Hiroshi Minaguchi, Tomonori Kigawa &  Hideshi Kobayashi

Authors
  1. Masumi Nozaki
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  2. Yuji Taketani
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  3. Hiroshi Minaguchi
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  4. Tomonori Kigawa
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  5. Hideshi Kobayashi
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Supported by grants from the Ministry of Education of Japan and the Ford Foundation

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Nozaki, M., Taketani, Y., Minaguchi, H. et al. Distribution of LHRH in the rat and mouse brain with special reference to the tanycytes. Cell Tissue Res. 197, 195–212 (1979). https://doi.org/10.1007/BF00233914

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