Skip to main content
SpringerLink
Log in

Somatic embryogenesis and plant regeneration from leaflets of peanut, Arachis hypogaea

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Somatic embryos were induced on peanut (Arachis hypogaea) leaflets from aseptically germinated embryo axes. Leaflet size influenced percent somatic embryogenesis; 5–8 mm long cut leaflets were superior to 2–3 mm long uncut leaflets. Maximum embryogenesis of 14.6% was obtained after a 15 d incubation on induction medium (modified MS with B5 vitamins, 30 g/l sucrose, 4 g/l Gel-Gro, 40 mg/l 2,4-D +0.2 mg/l kinetin) followed by transfer to a secondary medium with 5 mg/l 2,4-D+0.2 mg/l kinetin. Primary somatic embryos were fused along the axes with no distinct cotyledons, but secondary embryos had single axes with two cotyledons. Other treatments had lower percent embryogenesis, no secondary embryogenesis, and embryos with single axes with two cotyledons. Some somatic embryos converted into normal plants capable of greenhouse survival.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

MS:

Murashige and Skoog (1962) medium

B5:

Gamborg et al. (1968) B5 medium

2,4-D:

2,4-dichlorophenoxyacetic acid

BAP:

6benzylaminopurine

NAA:

1-naphthaleneacetic acid

References

  • Arcioni S, Mariotti D (1982) In: Fujiwara A (ed) Plant Tissue Culture, vol 1982, Japanese Association for Plant Tissue Culture Tokyo, pp 707–708

    Google Scholar 

  • Chen TH, Marowitch J, Thompson BG (1987) Plant Cell Tiss Org Cult 8:73–81

    Google Scholar 

  • Fraley RT, Rogers SG, Horsch RB (1988) CRC Crit Rev Plant Sci 4:1–46

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50:151–158

    CAS  PubMed  Google Scholar 

  • Gharyal PK, Maheshwari SC (1981) Naturwissenshaften 68:379–380

    Google Scholar 

  • Hanning GE, Conger BV (1982) Theor Appl Genet 63:155–159

    Google Scholar 

  • Hazra S, Sathaye SS, Mascarenhas AF (1989) Biotechnology 7:949–951

    Google Scholar 

  • Kysely W, Myers JR, Lazzeri PA, Collins GB, Jacobsen H (1987) Plant Cell Reports 6:305–308

    Google Scholar 

  • Kumar AS, Gamborg OL, Nabors MW (1988a) Plant Cell Reports 7:138–141

    Google Scholar 

  • Kumar AS, Gamborg OL, Nabors MW (1988b) Plant Cell Reports 7:322–325

    Google Scholar 

  • Lazzeri PA, Hildebrand DF, Collins GB (1985) Plant Molec Biol Rep 3:160–167

    Google Scholar 

  • McKently AH, Moore GA, Gardner FP (1991) Crop Sci 31:833–837

    Google Scholar 

  • McKently AH, Moore GA, Gardner FP (1989) Crop Sci 30:192–196

    Google Scholar 

  • Meijer EG, Brown DC (1987) Physiol Plant 69:591–596

    Google Scholar 

  • Mroginski LA, Kartha KK, Shyluk JP (1981) Can J Bot 59:826–830

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Narasimhulu SB, Reddy GM (1983) Plant Sci Lett 31:157–163

    Google Scholar 

  • Nisbet GS, Webb KJ (1990) Transformation in Legumes. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry: Legumes and Oilseed Crops, vol 10, part 1, Springer-Verlag Berlin, pp 38–48

    Google Scholar 

  • Nolan KE, Rose RJ, Gorst JR (1989) Plant Cell Reports 8:278–281

    Google Scholar 

  • Ozias-Akins P (1989) Plant Cell Reports 8:217–218

    Google Scholar 

  • Pederson GA (1986) Plant Sci 45:101–104

    Google Scholar 

  • Pittman RN, Banks DJ, Kirby JS, Mitchell ED, Richardson PE (1983) Peanut Sci 10:21–25

    Google Scholar 

  • Ranch JP, Oglesby L, Zielinski AC (1985) In Vitro Cell Devel Biol 21:653–658

    Google Scholar 

  • Sellars RM, Southward GM, Phillips GC (1990) Crop Sci 30:408–414

    Google Scholar 

  • Stalker HT, Moss JP (1987) Adv Agron 41:1–40

    Google Scholar 

  • Stamp JA, Henshaw GG (1987) Plant Cell Tiss Org Cult 10:227–233

    Google Scholar 

  • Walker KA, Sato SJ (1981) Plant Cell Tiss Org Cult 1:109–121

    Google Scholar 

  • Walker KA, Yu PC, Sato SJ, Jaworski EG (1978) Amer J Bot 65:654–659

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Horticulture Department, University of Georgia, 1111 Plant Science Building, 30602, Athens, GA, USA

    Charleen M. Baker & Hazel Y. Wetzstein

Authors
  1. Charleen M. Baker
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hazel Y. Wetzstein
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by G.C. Phillips

Rights and permissions

Reprints and permissions

About this article

Cite this article

Baker, C.M., Wetzstein, H.Y. Somatic embryogenesis and plant regeneration from leaflets of peanut, Arachis hypogaea . Plant Cell Reports 11, 71–75 (1992). https://doi.org/10.1007/BF00235256

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00235256

Keywords

Search

Navigation