Abstract
Somatic embryogenesis and plantlet formation were obtained from callus derived from the subapical region of spears of Asparagus cooperi Baker. Callus was obtained in Murashige and Skoog's medium supplemented with 1-naphthaleneacetic acid and kinetin. Increase in the concentration of potassium nitrate in subsequent subcultures resulted in the formation of embryos. Rapid multiplication of embryos was secured on transfer to a medium containing a different source of nitrogen and a low level (0.01 mg/1) of gibberellic acid. Media containing zeatin or gibberellic acid led to the formation of complete plantlets from embryos. Regenerated plants were cytologically and phenotypically stable.
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Abbreviations
- IBA:
-
Indole-3-butyric acid
- NAA:
-
1-Naphthaleneacetic acid
- 2ip:
-
(2-Isopentenyl) adenine
- BAP:
-
6-Benzylaminopurine, GA,, Gibberellin
- ABA:
-
Abscisic acid
- MS:
-
Murashige and Skoog (1962) medium
References
Abo El Nil NM (1977) Plant Sci Lett 9:259–264
Ammirato, PV (1983) Embryogenesis. In : Handbook of Plant Cell Culture Vol 1:82–123
Ammirato, PV (1987) In : Plant Tissue and Cell Culture, Alan R. Liss Inc 57–81
Chakravarty B, Sen S (1989) Plant Cell Tissue Organ Culture 19:71–75
Ghazi TD, Cheema HV, Nabors MW (1986) Plant Cell Reports 5:452–456
Ghosh B, Sen S (1989) Curr Sci 58(5): 256–257
Hassan El A, Debergh P (1987) Plant Cell Tissue Organ Culture 10:73–77
Haydu Z, Vasil Ik (1981) Theor Appl Genet 59:269–272
Ho W, Vasil Ik (1983) Ann Bot 51:719–726
Jha S, Sen S (1986) J Plant Physiol 124:431–439
Krikorian AD, Kann RP (1981) Ann Bot 47:679–686
Lu C, Vasil IK (1981) Theor Appl Genet 59:275–280
Lu C, Vasil IK (1982) Amer J Bot 69:77–81
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Raghavan V (1986) In : “Embryogenesis in Angiosperms. A Developmental and Experimental Study” Cambridge Univ Press pp 115–151
Reuther G (1977) Acta Hortic 78:217–224
Tazawa M, Reinert J (1969) Protoplasma 68:157–173
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Communicated by G. C. Phillips
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Ghosh, B., Sen, S. Plant regeneration through somatic embryogenesis from spear callus culture of Asparagus cooperi Baker. Plant Cell Reports 9, 667–670 (1991). https://doi.org/10.1007/BF00235353
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DOI: https://doi.org/10.1007/BF00235353