Summary
A quantitative morphological study of the changes in the dentate gyrus molecular layer in response to the removal of perforant path afferents was made utilizing electron microscopic techniques. Alterations in 1. the population of remaining afferents, 2. glial cells, and 3. granule cell dendrites are reported. The major observation was an increase in intact bouton density in the region of denervation which began at 5 days post-lesion and continued through 11 days post-lesion, the longest post-lesion survival time studied.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Blackstad, T.W.: Commissural connections of the hippocampal region in the rat with special reference to their mode of termination. J. comp. Neurol. 105, 417–537 (1956)
Fifkova, E.: Two types of terminal degeneration in the molecular layer of the dentate fascia following lesion of the entorhinal cortex. Brain Res. 96, 169–175 (1975)
Goodman, D.C., Horel, J.A.: Sprouting of optic tract projections in the brain stem of the rat. J. comp. Neurol. 127, 71–88 (1966)
Goodman, D.C., Bogdasarian, R.S., Horel, J.A.: Axonal sprouting of ipsilateral optic tract following opposite eye removal. Brain Behav. Evol. 8, 27–50 (1973)
Gottlieb, D.I., Cowan, W.M.: Autoradiographic studies of the commissural and ipsilateral associational connections of the hippocampus and dentate gyrus of the cat. J. comp. Neurol. 149, 393–422 (1973)
Guillery, R.W.: Light- and electron-microscopic studies in normal and degenerating axons. In: Contemporary Research Methods in Neuroanatomy (eds. W.J.H. Nauta and S.O.E. Ebbesson). Berlin-Heidelberg-New York: Springer 1970
Hjorth-Simonsen, A.: Origin and termination of the hippocampal perforant path in the rat studied by silver impregnation. J. comp. Neurol. 144, 215–232 (1972a)
Hjorth-Simonsen, A.: Projection of the lateral part of the entorhinal area to the hippocampus and fascia dentata. J. copm. Neurol. 146, 212–232 (1972b)
Karnovsky, M.H.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137A (1965)
Lund, R.D., Lund, J.S.: Synaptic adjustment after deafferentation of the superior colliculus of the rat. Science 171, 804–807 (1973)
Lynch, G., Matthews, D.A., Mosko, S., Parks, T., Cotman, C.W.: Induced acetylcholinesterase-rich layer in rat dentate gyrus following entorhinal lesion. Brain Res. 42, 311–318 (1972)
Lynch, G., Stanfield, B., Cotman, C.W.: Developmental differences in post-lesion axonal growth in the hippocampus. Brain Res. 59, 155–168 (1973a)
Lynch, G., Mosko, S., Parks, T., Cotman, C.W.: Relocation and hyperdevelopment of the dentate gyrus commissural system after entorhinal lesions in immature rats. Brain Res. 50, 174–178 (1973b)
Lynch, G., Deadwyler, S., Cotman, C: Postlesion axonal growth produces permanent functional connections. Science 180, 1364 (1973c)
Lynch, G., Rose, G., Gall, C., Cotman, C.: The response of the dentate gyrus to partial deafferentation. In: Golgi Centennial Symposium, Proceedings (ed. M. Santini). New York: Raven Press 1975
Lynch, G., Cotman, C.: The hippocampus as a model for studying anatomical plasticity in the adult brain. In: The Hippocampus, Vol. I (eds. R.L. Isaacson and K.H. Pribram). New York: Plenum Publishing Corporation 1976
Lynch, G., Gall, C., Rose, G., Cotman, C.: Changes in the distribution of the dentate gyrus associational system following unilateral or bilateral entorhinal lesion in the adult rat. Brain Res. 110, 57–71 (1976)
Lynch, G., Gall, C., Cotman, C.: Temporal parameters of post-lesion axonal growth. Exp. Neurol. 54, 179–183 (1977)
Matthews, D.A., Cotman, C., Lynch, G.: An electron microscopic study of lesioninduced synaptogenesis in the dentate gyrus of the adult rat. II. Reappearance of morphologically normal synaptic contacts. Brain Res. 115, 23–41 (1976)
Moore, R.Y., Bjorklund, A., Stenevi, U.: Plastic changes in the adrenergic innervation of the rat septal area in response to denervation. Brain Res. 33, 13–35 (1971)
Mosko, S., Lynch, G., Cotman C.W.: Distribution of the septal projections to the hippocampal formation of the rat. J. comp. Neurol. 152, 163–174 (1973)
Parnavelas, J.G., Lynch, G., Brecha, N., Cotman, C.W., Globus, A.: Spine loss and regrowth in hippocampus following deafferentation. Nature (Lond.) 248, 71–73 (1974)
Raisman, G.: Neuronal plasticity in the septal nuclei of the adult rat. Brain Res. 14, 25–48 (1969)
Raisman, G., Field, P.: A quantitative investigation of the development of collateral regeneration after partial deafferentation of the septal nuclei. Brain Res. 50, 241–264 (1973)
Rose, G., Cotman, C., Lynch, G.: Redistribution of astrocytes in the deafferented dentate gyrus. Brain Res. Bull. 1, 87–92 (1976)
Stenevi, U., Bjorklund, A., Moore, R.Y.: Growth of intact central adrenergic axons in the denervated lateral geniculate body. Exp. Neurol. 35, 290–299 (1972)
West, J.R., Deadwyler, S.A., Cotman, C.W., Lynch, G.S.: Time dependent changes in commissural field potentials in the dentate gyrus following lesions of the entorhinal cortex in adult rats. Brain Res. 97, 215–223 (1975)
Westrum, L.E., Black, R.G.: Changes in the synapses of the spinal trigeminal nucleus after ipsilateral rhizotomy. Brain Res. 11, 706–709 (1968)
Westrum, L.E.: Early forms of terminal degeneration in the spinal trigeminal nucleus following rhizotomy. J. Neurocytol. 2, 189–214 (1973)
Zimmer, J.: Ipsilateral afferents of the commissural zone of the fascia dentata demonstrated in decommissurated rats by silver impregnation. J. comp. Neurol. 142, 393–416 (1971)
Zimmer, J.: Extended commissural and ipsilateral projections in the postnatally de-entorhinated hippocampus and fascia dentata demonstrated in rats by silver impregnation. Brain Res. 64, 293–311 (1973)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lee, K.S., Stanford, E.J., Cotman, C.W. et al. Ultrastructural evidence for bouton proliferation in the partially deafferented dentate gyrus of the adult rat. Exp Brain Res 29, 475–485 (1977). https://doi.org/10.1007/BF00236185
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00236185