Summary
Rat kangaroo cells (PtK2) were studied with scanning and transmission electron microscopy in order to correlate shape changes during the cell cycle with the presence or absence of microvilli and stress fibers. During interphase, bundles of actin are prominent in the cytoplasm, and microvilli are localized over and around the centrally positioned nucleus. As mitosis begins, the interphase bundles of actin and the microvilli disappear, but the mitotic cells maintain a flattened shape. At metaphase the cell is still so flat that both the chromosomes and spindle apparatus are visible through the intact cell membrane. Microvilli reappear in late anaphase above the chromosomes and poles. Before cleavage begins, microvilli increase in number until they cover the apical surface of the cell. At the same time, the cell increases in height so that the chromosomes and mitotic apparatus can no longer be detected through the cell membrane. During cleavage, microvilli continue to cover the cell in a uniform manner but become greatly diminished in number after cytokinesis is completed and the cells flatten and enter interphase. It is suggested that the microvilli organize a network of actin filaments which interact with cortical myosin to produce the cell rounding prior to cleavage.
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References
Buck RC, Krishan A (1964) Site of membrane growth during cleaving of amphibian epithelial cells. Exp Cell Res 38:426–428
Buckley IK, Porter KR (1967) Cytoplasmic fibrils in living cells. Protoplasma 64:349–380
Burgess DR, Schroeder TE (1977) Polarized bundles of actin filaments within microvilli of fertilized sea urchin eggs. J Cell Biol 74:1032–1037
Erickson CA, Trinkhaus TP (1976) Microvilli and blebs as sources of reserve surface membrane during cell spreading. Exp Cell Res 99:375–384
Follett EAC, Goldman RD (1970) The occurrence of microvilli during spreading and growth of BHK 21/C13 fibroblasts. Exp Cell Res 59:124–136
Hiramoto Y (1970) Rheology of sea urchin eggs. Biorheology 6:201–234
Ishikawa H, Bischoff R, Holtzer H (1969) Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J Cell Biol 43:312–328
Knutton S, Summer MCB, Pasternak CA (1975) Role of microvilli in surface changes of synchronized P815Y mastocytoma cells. J Cell Biol 66:568–576
Lazarides E (1975) Immunofluorescence studies on the structure of actin filaments in tissue culture cells. J Histochem Cytochem 23:570–528
Mitchison JM, Swann MM (1955) The mechanical properties of the cell surface. III The sea-urchin egg from fertilization to cleavage. J Exp Biol 32:734–750
Mooseker MS (1976) Brush border motility. Microvillar contraction in Triton-treated brush borders isolated from intestinal epithelium. J Cell Biol 71:417–433
Mooseker MS, Tilney L (1975) Organization of an actin filament-membrane complex. J Cell Biol 67:725–743
Mooseker MS, Pollard TD, Fujiwara K (1978) Characterization and localization of myosin in the brush border of intestinal epithelial cells. J Cell Biol 79:444–453
Paweletz N, Schroeter D (1974) Scanning electron microscopic observation on cells grown in vitro II. Hela cells in mitosis. Cytobiologie 8:238–246
Porter K, Prescott D, Frye J (1973) Changes in surface morphology of Chinese hamster ovary cells during the cell cycle. J Cell Biol 57:815–836
Sanger JW (1975a) Changing patterns of actin localization during cell division. Proc Natl Acad Sci 72:1913–1916
Sanger JW (1975b) The presence of actin during chromosomal movement. Proc Natl Acad Sci 72:2451–2455
Sanger JW, Sanger JM (1976) Actin localization during cell division. Cold Spring Harbor Conf on Cell Prolif 3:1295–1316
Sanger JW, Sanger JM (1979) The cytoskeleton and cell division. Meth Achiev Exp Pathol 8:110–142
Sanger JW, Sanger JM, Gwinn J (1979) Actin and the mitotic spindle. In: Pepe FA, Sanger JW, Nachmias VT (eds) Motility in cell function. Academic Press, New York, pp 313–323
Schroeder TE (1978) Microvilli on sea urchin eggs: a second burst of elongation. Dev Biol 64:342–346
Schroeder TE, Strickland DL (1974) Ionophore A23187, calcium and contractility in frog eggs. Exp Cell Res 83:139–142
Strangeways TSP (1922) Observations on the changes seen in living cells during growth and division. Proc R Soc B 94:137–141
Vasiliev JM, Gelfand IM (1977) Mechanisms of morphogenesis in cell cultures. Int Rev Cytol 50:159–274
Weber K (1976) Visualization of tubules containing structures by immunofluorescence microscopy; cytoplasmic microtubules, mitotic figures and vinblastine-induced paracrystals. Cold Spring Harbor Conf Cell Prolif 3:403–417
Weber K, Gröschel-Stewart U (1974) Antibody to myosin: the specific visualization of myosincontaining filaments in nonmuscle cells. Proc Natl Acad Sci 71:4561–4564
Zimmerman AM, Landau JV, Marsland D (1957) Cell division: a pressure-temperature analysis of the effects of sulfhydryl reagents on the cortical plasmagel structure and furrowing strength of dividing eggs (Arbacia and Chaetopterus). J Cell Comp Physiol 49:395–435
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Sanger, J.W. Surface and shape changes during cell division. Cell Tissue Res. 209, 177–186 (1980). https://doi.org/10.1007/BF00237624
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DOI: https://doi.org/10.1007/BF00237624