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Ultrastructural responses of pancreatic β cells to metabolic alkalosis

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Summary

The ultrastructural changes in pancreatic β cells were studied following glucose-induced insulin secretion in vitro, at two different extracellular pH (7.4 and 7.8). The pancreata perfused at pH 7.4 exhibited a biphasic insulin response to glucose challenge together with signs of increased emiocytotic activity and numerous microtubules in the β cells. Conversely, the pancreata perfused at pH 7.8 showed a significant decrease in insulin secretion, and their β cells revealed scarce emiocytotic images and a marked increase of intracellular granulolysis. These results represent the ultrastructural correlate of the reduced insulin secretion produced by metabolic alkalosis in the perfused rat pancreas.

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Authors and Affiliations

  1. Centro de Endocrinología Experimental y Aplicada (CENEXA) e Instituto de Embriologia, Biología e Histología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina

    César L. Gómez Dumm, Oscar R. Rebolledo &  Juan José Gagliardino

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  1. César L. Gómez Dumm
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  2. Oscar R. Rebolledo
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  3. Juan José Gagliardino
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Additional information

The authors wish to thank Mrs. Elma P. de Gagliardino and Mrs. Susana Rivas for excellent technical assistance.

This research was partially supported by funds from CONICET and CIC, Pcia de Bs.As. C.L. Gómez Dumm, O.R. Rebolledo and J.J. Gagliardino are members of Carrera del Investigador del CONICET (Argentina)

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Gómez Dumm, C.L., Rebolledo, O.R. & Gagliardino, J.J. Ultrastructural responses of pancreatic β cells to metabolic alkalosis. Cell Tissue Res. 201, 159–163 (1979). https://doi.org/10.1007/BF00238055

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  • DOI: https://doi.org/10.1007/BF00238055

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