Abstract
The aim of this study was to examine the effects of chronic low frequency stimulation on the lactate transport across the plasma membrane of the tibialis anterior (TA) muscle of the rat. Stimulating electrodes were implanted on either side of the peroneal nerve in one hindlimb. Chronic stimulation (10 Hz, 50 psec bursts, 24 h/day) commenced 7 days after surgery, and were continued for 7 days. Animals were then left for 24 h, and thereafter muscles were obtained. Cytochrome C-oxidase activity was increased 1.9-fold in the stimulated TA compared to the control TA (p < 0.05). Lactate transport (zero-trans) was measured in giant sarcolemmal vesicles obtained from the chronically stimulated TA and the control TA. At each of the concentrations used in these studies a significant increase in lactate transport was observed: 2.8-fold increase at 1 mM lactate p < 0.05); 2-fold increases at both 30 mM and 50 mM lactate p < 0.05). These studies have shown that lactate transport capacity is markedly increased in response to chronic muscle contraction.
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References
Juel C: Muscle lactate transport studied in sarcolemmal giant vesicles. Biochim Biophys Acta 1065: 15–20, 1991
McDermott JC, Bonen A: Lactate transport by skeletal muscle sarcolemmal vesicles. Mol Cell Biochem 122: 113–121, 1993
Roth DA, Brooks GA: Lactate transport is mediated by a membranebound carrier in rat skeletal muscle sarcolemmal membranes. Arch Biochem Biophys 279: 377–385, 1990
Roth DA, Brooks GA: Lactate and pyruvate transport is dominated by by a pH gradient-sensitive carrier in rat skeletal muscle sarcolemmal vesicles. Arch Biochem Biophys 279: 386–394, 1990
Brown MA, Brooks GA: Trans-acceleration of lactate transport from rat sarcolemmal membrane vesicles. Arch Biochem Biophys 313: 22–28, 1994
McCullagh KJA, Bonen A: L(+)-lactate binding to a protein in rat skeletal muscle plasma membranes. Can J Appl Physiol 20: 112–124, 1995
Allen PJ, Brooks GA: Partial purification and reconstitution of the sarcolemmal lactate carrier from rat skeletal muscle. Biochem J 303: 207–212, 1994
Hennig R, Lomo T: Firing patterns of motor units in normal rats. Nature 314: 164–166, 1985
Bonen A, McCullagh KJA: Effects of exercise on lactate transport into mouse skeletal muscles. Can J Appl Physiol 19: 275–285, 1994
Juel C, Honig A, Pilegaard H: Muscle lactate transport studied in sarcolemmal giant vesicles: dependence on fiber type and age. Acta Physiol Scand 143: 361–365, 1991
McDermott JC, Bonen A: Endurance training increases skeletal muscle lactate transport. Acta Physiol Scand 147: 323–327, 1993
Pilegaard H, Juel C, Wibrand F: Lactate transport studied in sarcolemmal giant vesicles from rats: effects of training. Am J Physiol 264 (Endocrinol Metab): E1566-E160, 1993
Roth DA, Brooks GA: Training does not affect zero-trans lactate transport across minced muscle sarcolemmal vesicles. J Appl Physiol 75: 1559–1565, 1993
Enoka RM: Muscle strength and its development. New Perspectives. Sports Med 6: 146–168, 1988
McCullagh KJA, Bonen A: Reduced lactatetransport in denervated rat skeletal muscle. Am J Physiol (Reg Int Comp Physiol) 268: R884-R888, 1995
Kraus WE, Torgan CE, Taylor DA: Skeletal muscleadaptation to chronic low frequency stimulation. In: J.O. Holloszy (ed.). Reviews in Exercise and Sport Sciences 1994: 313–360
McDermott JC, Bonen A: Lactate transport in rat sarcolemmal vesicles and intact skeletal muscle, and after muscle contraction. Acta Physiol Scand 151: 17–28, 1994
Takahashi M, Hood DA: Chronic stimulation-induced changes in mitochondria and performance in rat skeletal muscle. J Appl Physiol 74: 934–941, 1993
Takahashi M, Rana A, Hood DA: Portable elentrical stimulator for use in small animals. J Appl Physiol 74: 942–945, 1993
Ploug T, Wojtaszewski J, Kristiansen S, Hespel P, Galbo H, Richter EA: Glucose transport and transporters in muscle giant vesicles: differential effects of insulin and contractions. Am J Physiol 264: E270-E278, 1993
Hood DA, Zak R, Pette D: Chronic stimulation of rat skeletal muscle induces coordinate increases in mitochondrial and and nuclear mRNAs of cytochrome-C-oxidase subunits. Europ J Biochem 179: 275–280, 1989
Williams RS, Garcia-Moll M, Mellor J, Salmons S, Harlan W: Adaptation of skeletal muscle to increased contractile activity. J Biol Chem 262: 2764–2767, 1987
Juel C, Kristiansen S, Pilegaard H, Wojtaszewski J, Richter EA: Kinetics of lactate transport in sarcolemmal giant vesicles obtained from human skeletal muscle. J Appl Physiol 76: 1031–1036, 1994
Wojtaszewski JS, Kristiansen S, Ploug T, Hespel P, Richter EA: A method for production of skeletal muscleplasma membrane giant vesicles for the study of glucose transport. Acta Physiol Scand 143: P18, 1991
Pilegaard H, Bangsbo J, Richter EA, Juel C: Lactate transport studied in sarcolemmal giant vesicles from human muscle biopsies: relation to training status. J Appl Physiol 77: 1858–1862, 1994
Pette D, Vrbova G: Adaptation of skeletal muscle fibers to chronic electrical stimulation. Rev Physiol Biochem Pharmacol 120: 116202, 1992
Garcia CK, Goldstein JL, Pathak RK, Anderson GW, Brown MS: Molecular characterization of a membrane transporter for lactate, pyruvate, and other monocarboxylates: implications for the Cori cycle. Cell 76: 865–873, 1994
Garcia CK, Li X, Luna J, Francke U. cDNA Cloning of the Human Monocarboxylate Transporter 1 and Chromosomal Localization of the SLC16A1 Locus to 1p13.2-pl2. Genomica 23: 500–503, 1994
Jennings ML, Adams-Lackley M: A rabbit erythrocyte membrane protein associated with with L-lacatate transport. J Biol Chem 257: 12866–12871, 1982
Wang X, Poole RC, Halestrap AP, Levi AJ: Characterization of the inhibition by stilbene disulphonates and phloretin of lactate and pyruvate transport into rat and guinea-pig cardiac myocytes suggests the presence of two kinetically distinct carriers in heart cells. Biochem J 290: 249–258, 1993
Wang X, Levi AJ, Halestrap AP: Kinetics of the sarcolemmal lactate carrier in single heart cells using BCECF to measure pHi. Am J Physiol 267: H1759-H1769, 1994
Garcia CK, Brown MS, Pathak RK, Goldstein JL: cDNA cloning of MCT2, a second monocarboxylate transporter expressed in different cells than MCI 1. J Biol Chem 270: 1843–1849, 1995
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McCullagh, K.J.A., Juel, C., O'Brien, M. et al. Chronic muscle stimulation increases lactate transport in rat skeletal muscle. Mol Cell Biochem 156, 51–57 (1996). https://doi.org/10.1007/BF00239319
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DOI: https://doi.org/10.1007/BF00239319