Summary
For a detailed analysis of the oxygen supply of hippocampal slices, tissue PO2 (Pt,o 2) was recorded polarographically in the neural layers of thick and thin slice preparations from the guinea pig. The experiments showed that the Pt,o2-gradients were extremely steep in the outer zones of vital slices. In an air equilibrated salt solution the surface PO2 was reduced to less than 50% within ca. 25 μm. Minimum values were measured at a depth of ca. 150 μm. A rise of temperature lowered the oxygen supply in the deeper layers of the excised tissue. An elevation of the surface PO2 hardly improved Pt,o 2 in the deep structures, because the O2-consumption of the hippocampal slices increased with rising PO2.
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Acker H, Keller H-P, Lubbers DW, Bingmann D, Schulze H, Caspers H (1973) The relationship between neuronal activity of chemoreceptor fibers and tissue PO2 and blood pressure. Pfluegers Arch 343: 287–296
Acker H, Heinrich R, Lübbers DW, Bingmann D, Caspers H (1977) The relation between tissue PO2 in the cat carotid body and chemoreceptor activity under hyperoxic conditions. Proc of the International Union of Physiological Sciences 13: 5
Andersen P, Bliss TVP, Skrede KK (1971) Lamellar organization of hippocampal excitatory pathways. Exp Brain Res 13: 222–238
Bak I, Misgeld U, Weiler M, Morgen E (1980) The preservation of nerve cells in rat neostriatal slices maintained in vitro: a morphological study. Brain Res 197: 341–353
Bingmann D, Kolde G (1979) Burst activity elicited in hippocampal slices by a rise of PO2. Pfluegers Arch 382: 38
Brierly JB (1976) Cerebral hypoxia. In: Blackwood W, Corsellis J (eds) Greenfield's Neuropathology. Arnold, London pp 43–85
Burg von R, Lijoi A, Smith C (1979) Oxygen consumption of rat tissue slices exposed to methylmercury in vitro. Neurosci Lett 14: 309–314
Clark DK, Erdmann W, Halsey JH, Strong F (1978) Oxygen diffusion, conductivity and solubility coefficients in the micro area of the brain. In: Silver IA, Erecinska M, Sicher H (eds) Oxygen transport to tissue III. Plenum, New York pp 697–704
Dingledine R, Dodd J, Kelly JS (1980) The in vitro brain slice as a useful neurophysiological preparation for intracellular recording. J Neurosci Meth 2: 323–362
Farr DA, Fuhrmann FA (1965) Role of diffusion of oxygen in the respiration of tissues at different temperatures. J Appl Physiol 20: 637–646
Fuhrmann FA, Field J (1945) Factors determining the metabolic rate of excises liver tissue. Arch Biochem 6: 337–349
Ganfield RA, Nair P, Whalen WJ (1970) Mass transfer, storage and utilization of O2 in cat cerebral cortex. Am J Physiol 219: 814–821
Garthwaite J, Woodhams PL, Collius MJ, Balazs R (1979) On the preparation of brain slices: morphology and cyclic nucleotides. Brain Res 173: 373–377
Grote J, Süsskind R, Vaupel P (1978) Oxygen diffusion constants D and K of tumor tissue and their temperature dependence: In: Silver IA, Erecinska M, Bicher H (eds) Oxygen transport to tissue III. Plenum, New York pp 361–365
Hansen AJ, Jahnsen H, Hounsgaard JB (1979) Influence of hypoxia on hippocampal nerve cells in vitro. Acta Physiol Scand [Suppl] 473: 55
Harvey JA, Scholfield CN, Brown DA (1974) Evoked surface- positive potentials in isolated mammalian olfactory cortex. Brain Res 76: 235–245
Jenkins LW, Povlishock JT, Becker DP, Miller JD, Sullivan HG (1979) Complete cerebral ischemia. An ultrastructural study. Acta Neuropathol (Berl) 48: 113–125
Lehmenkühler A, Caspers H, Speckmann E-J (1976) A method for simultaneous measurement of bioelectric activity and local tissue PO2 in the CNS. In: Grote J, Reneau D, Thews G (eds) Oxygen transport to tissue II. Plenum, New York pp 3–7
Lehmenkühler A, Zidek W, Staschen M, Caspers H (1981) Cortical pH and pCa in relation to DC potential shift during spreading depression and asphyxiation. In: Vyklicky L, Sykova E, Huik P (eds) Ion-selective microelectrodes and their use in excitable tissues. Plenum, New York pp 225–230
Lensing J, Sasse L (1978) Gleichzeitiges Messen des Sauerstoff- partialdrucks und bioelektrischer Potentiale in lebendem Gewebe. Elektronik 4: 91–94
Lierse W (1963) Die Kapillardichte im Rhinencephalon verschiedener Wirbeltiere und des Menschen. In: Bargmann W, Schade JP (eds) Progress in brain research, Vol. 3, The rhinencephalon and related structures. Elsevier, Amsterdam pp 230–236
Lipton P, Wittingham TS (1979) The effect of hypyoxia on evoked potentials in the vitro hippocampus. J Physiol (London) 287: 427–438
Lübbers DW (1973) Local tissue PO2: its measurement and meaning In: Kessler M, Bruley DF, Clark LC, Lubbers DW, Silver IA, Strauss J (eds) Oxygen suppley. Urban & Schwarzenberg, München Berlin Wien pp 151–155
Lynch G, Schubert P (1980) The use of in vitro brain slices for multidisciplinary studies of synaptic function. Ann Rev Neurosci 3: 1–22
Metzger H, Heuber-Metzger S, Steinacker A, Strüber J (1980) Staining PO2 measurement sites in the rat brain cortex and quantitative morphometry of the surrounding capillaries. Pfluegers Arch 388: 21–27
Morris ME (1974) Hypoxia and extracellular potassium activity in the guinea pig cortex. Can J Physiol Pharmacol 5: 872–882
Scholfield CN (1978) Electrical properties of neurons in the olfactory cortex slices in vitro. J Physiol (London) 275: 535–546
Schwarzkroin PA (1975) Characteristics of CA 1 neurons recorded intracellularly in the hippocampal in vitro slice preparation. Brain Res 85: 423–436
Silver JA (1977) Ion fluxes in hypoxic tissues. Microvasc Res 13 409–420
Skredde KK, Westgaard RH (1971) The transverse hippocampal tissue: a well defined cortical structure maintained in vitro. Brain Res 35: 589–593
Smith RH, Guilbeau EJ, Renau EY (1977) The oxygen tension field within a discrete volume of cerebral cortex. Microvasc Res 13: 233–244
Speckmann E-J, Caspers H (1974) The effects of O2 and CO2 tensions in the nervous tissue on neuronal activity and DC potentials. In: Rémond A (ed) Handbook of Electroence-phalography and Clinical Neurophysiology II C. Elsevier, Amsterdam London New York pp 71–89
Tayler TJ (1980) Brain slice preparation: hippocampus. Brain Res Bull 5: 391–403
Thews G (1960) Ein Verfahren zur Bestimmung des O2-Diffusionskoeffizienten, der O2-Leitfähigkeit und des Löslichkeitskoeffizienten im Gehirngewebe. Pfluegers Arch 271: 227–244
Tsacopoulos M, Lehmenkühler A (1977) A double-barrelled Pt-microelectrode for simultaneous measurement of PO2 and bioelectrical activity in excitable tissues. Experientia 33: 1337–1338
Tsacopoulos M, Poitry S, Borsellino A (1981) Diffusion and consumption of oxygen in the superfused retina of the drone in darkness. J Gen Physiol 77: 601–628
Vyskocil F, Kriz N, Bures J (1972) Potassium-selective microelectrodes used for measuring the extracellular brain potassium during spreading depression and anoxic depolarization in rats. Brain Res 39: 255–259
Warburg O (1923) Versuche an überlebendem Carcinomgewebe. Biochem Z 142: 317–333
Yamamoto C (1972) Intracellular study of seizure-like afterdischarges elicited in thin hippocampal sections in vitro. Exp Neurol 35: 154–164
Yamamoto C, Kurokawa M (1970) Synaptic potentials recorded in brain slices and their modification by changes in the level of tissue ATP. Exp Brain Res 10: 159–170
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Dedicated to Professor Dr. H. Caspers on the occasion of his 60th birthday
Supported by DFG (Sp 108/6)
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Bingmann, D., Kolde, G. PO2-profiles in hippocampal slices of the guinea pig. Exp Brain Res 48, 89–96 (1982). https://doi.org/10.1007/BF00239575
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DOI: https://doi.org/10.1007/BF00239575