Abstract
A method is described for measuring the cell volume of the unicellular green alga Chlorococcum submarinum, which depends on measurements of bromide concentration before and after disruption of the cells by ammonium hydroxide. Simultaneous equations are derived, which along with direct determination of cell water weight, allow the calculation of the intracellular volume in three different ways. The volumes calculated are in agreement indicating the validity of the method. The cell volumes and internal concentrations of glycerol, proline, potassium and sodium were determined for algae adapted to three salinities, 0.1, 0.5 and 1.0 M NaCl. The results showed that glycerol was the major internal solute and that the total measured solutes balanced the external osmotic pressure at all three salinities.
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Abbreviations
- DMSO:
-
dimethyl sulphoxide
- Hepes:
-
N-[2-hydroxyethyl]piperazine-N-2-ethane sulfonic acid
- TCA:
-
trichloroacetic acid
- Tris:
-
tris[hydroxymethyl]aminoethane
References
Bates IS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 29: 205–207
Ben-Amotz A, Avron M (1978) On the mechanism of osmoregulation in Dunaliella. In: Caplan SR, Ginzburg M (eds) Energetics and structure of halophilic microorganisms. Elsevier/North Holland, Amsterdam, pp 529–541
Ben-Amotz A, Avron M (1983) Accumulation of metabolites by halotolerant algae and its industrial potential. Annu Rev Microbiol 37: 95–119
Blackwell JR, Cox EJ, Gilmour DJ (1991) The morphology and taxonomy of Chlorococcum submarinum (Chlorococcales) isolated from a tidal rockpool. Br Phycol J 26: 133–139
Blackwell JR, Gilmour DJ (1989) Determination of volume of Dunaliella cells by lithium dilution measurements and derivation of internal solute concentrations. J Exp Bot 40: 795–802
Blackwell JR, Gilmour DJ (1991) Stress tolerance of the tidal pool chlorophyte, Chlorococcum submarinum. Br Phycol J 26: 141–147
Delieu T, Walker DA (1972) An improved cathode for the measurement of photosynthetic oxygen evolution by isolated chloroplasts. New Phytol 71: 201–225
Gilmour DJ (1982) The effect of osmotic stress on photosynthesis in the unicellular green alga Dunaliella tertiolecta. PhD thesis, University of Glasgow, Faculty of Science
Ginzburg M (1981) Measurements of ion concentrations and fluxes in Dunaliella parva. J Exp Bot 32: 321–332
Katz A, Avron M (1985) Determination of intracellular osmotic volume and sodium concentration in Dunaliella. Plant Physiol 78: 817–820
Raven JA, Smith FA, Smith SE (1980) Ions and osmoregulation. In: Rains DW, Valentine RC, Hollander A (eds) Genetic engineering of osmoregulation: impact on plant productivity for food, chemicals and energy. Plenum Press, New York, pp 108–118
Rottenberg H (1979) The measurement of membrane potential and ΔpH in cells, organelles and vesicles. Methods Enzymol 55: 547–569
Strickland JDH, Parsons TR (1968) A practical handbook of seawater analysis. Fisheries Research Board of Canada, Bulletin 167
Weast RC, Astle MJ (1982) CRC handbook of chemistry and physics, 63rd edn. CRC Press, Boca Raton, Florida
Yancey PH, Clark ME, Hand SC, Bowlus RD, Somero GN (1982) Living with water stress: evolution of osmolyte systems. Science 217: 1214–1222
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Blackwell, J.R., Gilmour, D.J. Determination of intracellular volume and internal solute concentrations of the green alga Chlorococcum submarinum . Arch. Microbiol. 157, 80–85 (1991). https://doi.org/10.1007/BF00245340
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DOI: https://doi.org/10.1007/BF00245340