Summary
Progeny from crosses of Nicotiana langsdorffii with gamma irradiated pollen of Nicotiana alata ‘Crimson Bedder’ showed skewed segregation in the F2 favoring the maternal parent. This is probably not gene transfer in a strict sense, rather just an extreme case of reduced transmission of irradiated chromosomes, leading to massive overrepresentation of maternal genes. Gene transfer or mutational loss may explain some anomalous F1 plants. Segregation in the F2 progeny showed the presence of several genes from the irradiated pollen. Crosses of Nicotiana sylvestris, N. plumbaginifolia N. paniculata, and Petunia parodii with irradiated pollen from N. alata and Petunia hybrida showed no evidence of gene transfer, nor did experiments with irradiated mentor pollen. This indicates that gene transfer with irradiated pollen between non-crossing species or between species giving sterile hybrids is probably a rare phenomenon.
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References
Brewbaker JL, Emery GC (1962) Pollen radiobotany. Radiat Bot 1:101–154
Brown SW, Cave MS (1954) The detection and nature of dominant lethals in Lilium. I. Effects of X rays on the heritable component and functional ability of the pollen grain. Am J Bot 41:455–469
Caligari PDS, Ingram NR, Jinks JL (1981) Gene transfer in Nicotiana rustica by means of irradiated pollen. I. Unselected progenies. Heredity 47:17–26
Davies DR (1981) Gene transfer in plants. Nature 291:531–532
Davies DR (1984) Pollen irradiation and the transfer of maternal genes in Pisum sativum. Theor Appl Genet 67:245–248
Dellaert LMW (1980) Segregation frequencies of radiationinduced viable mutants in Arabidopsis thaliana (L.) Heynh. Theor Appl Genet 57:137–143
East EM (1930) The origin of the plants of maternal type which occur in connection with interspecific hybridizations. Proc Natl Acad Sci USA 16:377–380
Gaul H (1963) Mutationen in der Pflanzenzüchtung. Z Pflanzenzücht 50:194–307
Goodspeed TH (1954) The genus Nicotiana. Chronica Botanica, Waltham Mass
Jinks JL, Caligari PDS, Ingram NR (1981) Gene transfer in Nicotiana rustica using irradiated pollen. Nature 291:586–588
Katayama Y, Adachi T (1969) Studies on the haploidy in relation to plant breeding IV Parthenogenetic development induced by alien pollen. Seiken Ziho 21:31–35
Nettancourt D de (1977) Incompatibility in angiosperms. Springer, Berlin Heidelberg New York
Pandey KK (1975) Sexual transfer of specific genes without gametic fusion. Nature 256:310–313
Pandey KK (1980 a) Further evidence for egg transformation in Nicotiana. Heredity 45:15–29
Pandey KK (1980 b) Parthenogenetic diploidy and egg transformation induced by irradiated pollen in Nicotiana. NZJ Bot 18:203–207
Pandey KK (1983) Evidence for gene transfer by the use of sublethally irradiated pollen in Zea mays and theory of occurrence by chromosome repair through somatic recombination and gene conversion. Mol Gen Genet 191:358–365
Pandey KK, Phung M (1982) ‘Hertwig effect’ in plants: induced parthenogenesis through the use of irradiated pollen. Theor Appl Genet 62:295–300
Powell W, Caligari PDS, Hayter AM (1983) The use of pollen irradiation in barley breeding. Theor Appl Genet 65:73–76
Shizukuda N, Yamamoto K, Nakajima T (1983) Sexual transfer of an incomplete chromosome complement from Nicotiana tabacum L. to N. rustica L. Jpn J Breed 33:15–22
Snape JW, Parker BB, Simpson E, Ainsworth CC, Payne PI, Law CN (1983) The use of irradiated pollen for differential gene transfer in wheat (Triticum aestivum). Theor Appl Genet 65:103–111
Stadler LJ, Roman H (1948) The effect of X-rays upon mutation of the gene A in maize. Genetics 33:273–303
Virk DS, Dhahi SJ, Brumpton RJ (1977) Matromorphy in Nicotiana rustica. Heredity 39:287–295
Zamir D (1983) Pollen irradiation in tomato: minor effects on enzymic gene transfer. Theor Appl Genet 66:147–151
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Engvild, K.C. Pollen irradiation and possible gene transfer in Nicotiana species. Theoret. Appl. Genetics 69, 457–461 (1985). https://doi.org/10.1007/BF00251084
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DOI: https://doi.org/10.1007/BF00251084