Summary
Several types of filamentous microorganisms were observed and identified in samples of poorly settling (bulking) activated sludge. The major types encountered and the frequency (percentage) of appearance in the total of all treatment plants sampled were: Eikelboom type 0041 (60), type 1701 (45), Haliscomenobacter hydrossis (35), type 021N (30), Thiothrix spp. (20), and Sphaerotilus natans (20). Isolation techniques and culture media were developed and used to recover 42 axenic strains of filamentous bacteria from sludge samples collected. The isolates were identified as strains of Thiothrix, Beggiatoa, S. natans, and Eikelboom types 021N, 1701, 0041, and 0803. Nutritional and differential characterization of the bacteria was important to the differentiation of groups which could not be easily distinguished on the basis of morphology. Although certain treatment plant operating parameters (organic loading) seemed associated with the presence of specific filamentous organism types, possible interaction among factors precluded definite establishment of a cause and effect relationship for most of the treatment plant characteristics and organisms observed.
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References
Buchanan RE, Gibbons NE (eds) (1974) Bergey's manual of determinative bacteriology, 8th edn. The Williams and Wilkins Co., Baltimore
Deinema MH, Henstra S, Werdmuller von Elgg E (1977) Structural and physiological characteristics of some sheathed bacteria. Antonie van Leeuwenhoek. J Microbiol Serol 43:19–29
Dondero NC, Phillips RA, Heukelekian H (1961) Isolation and preservation of cultures of sphaerotilus. Appl Microbiol 9:219–227
Eikelboom DH (1975) Filamentous organisms observed in activated sludge. Water Res 9:365–388
Eikelboom DH (1977) Identification of filamentous organisms in bulking activated sludge. Prog Water Technol 8:151–161
Eikelboom DH, van Buijsen HJJ (1981) Microscopic sludge investigation manual. Report A 94A. TNO Research Institute for Environmental Hygiene, Delft, The Netherlands
Farquhar GJ, Boyle WC (1971) Occurrence of filamentous micro-organisms in activated sludge. J Water Pollut Control Fed 43:779–798
Farquhar GJ, Boyle WC (1972) Control of Thiothrix in activated sludge. J Water Pollut Control Fed 44:14–24
Larkin JM, Shinabarger DL (1983) Characterization of Thiothrix nivea. Int J Syst Bacteriol 33:841–846
Lau AO, Strom PF, Jenkins D (1984) The competitive growth of floc-forming and filamentous bacteria: A model for activated sludge bulking. J Water Pollut Control Fed 56:52–61
Mulder EG, Deinema MH (1981) The sheathed bacteria. In: Starr MP, Truper HG, Balows A, Schlegel HG (eds) The procaryotes. Springer-Verlag, New York, p 425
Pipes WO (1978) Microbiology of activated sludge bulking. Adv Appl Microbiol 24:85–127
Poffe RJ, Vanderlyden J, Verachtert H (1979) Characterization of a Leucothrix-type bacterium causing sludge bulking during petrochemical wasterwater treatment. European J Appl Microbiol Biotech 8:229–235
Salcher O, Scheff G, Senghas E, Trick I, Lingens F (1982) The nutritional pattern of filamentous bacteria isolated from bulking sludge. Bacteriol Hyg [Orig C] 3:450–456
Scheff G, Salcher O, Lingens F (1984) Trichococcus flocculiformis. gen. nov. sp. nov. A new gram-positive filamentous bacterium isolated from bulking sludge. Appl Microbiol Biotechnol 19:114–119
Skerman VBD (1967) A guide to the identification of the genera of bacteria, 2nd ed. Williams and Wilkins Co., Baltimore
Slijkhuis H (1983) Microthrix parvicella, a filamentous bacterium isolated from activated sludge: Cultivation in a chemically defined medium. Appl Environ Microbiol 46:832–839
Smibert RM, Krieg NR (1981) General characteriation. In: Gerhardt P (ed) Manual of methods for general bacteriology. American Society for Microbiology, Washington DC, p 409
Strom PF, Jenkins D (1984) Identification and significance of filamentous microorganisms in activated sludge. J Water Pollut Control Fed 56:449–459
Trick I, Lingens F (1984) Characterization of Herpetosiphon spec. — A gliding filamentous bacterium from bulking sludge. Appl Microbiol Biotechnol 19:191–198
Trick I, Salcher O, Lingens F (1984) Characterization of filament forming Bacillus strains isolated from bulking sludge. Appl Microbiol Biotechnol 19:120–124
van Veen WL (1973) Bacteriology of activated sludge, in particular the filamentous bacteria. Antonie van Leeuwenhoek. J Microbiol Serol 39:139–205
van Veen WL, Bulder EG, Deinema MH (1978) The Sphaerotilus-Leptothrix group of acteria. Microbiol Rev 42:329–356
Wagner F (1982) Study of the causes and prevention of sludge bulking in Germany. In: Chambers B, Tomlinson EK (eds) Bulking of activated sludge: Preventative and remedial methods. Ellis Horwood LTD, Chichester, p 29
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Williams, T.M., Unz, R.F. Isolation and characterization of filamentous bacteria present in bulking activated sludge. Appl Microbiol Biotechnol 22, 273–282 (1985). https://doi.org/10.1007/BF00252030
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DOI: https://doi.org/10.1007/BF00252030