Summary
Pachysolen tannophilus contains — in addition to an NADPH-linked xylose reductase — a separate NADH-linked one, in this respect differing from the yeast Pichia stipitis. Both enzyme proteins can conveniently be separated from each other by either ion exchange chromatography or chromatofocusing.
References
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Ditzelmüller, G., Kubicek-Pranz, E.M., Röhr, M. et al. NADPH-specific and NADH-specific xylose reduction is catalyzed by two separate enzymes in Pachysolen tannophilus . Appl Microbiol Biotechnol 22, 297–299 (1985). https://doi.org/10.1007/BF00252033
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DOI: https://doi.org/10.1007/BF00252033