Summary
The formation and location of glucose oxidase was studied in Aspergillus niger, which was pregrown under citric acid producing conditions. Glucose oxidase could be “de novo” induced by a shift in pH from 1.7 to 5.5. The induction required the intracellular presence of either glucose or glucose-6-phosphate. Glucose oxidase so produced was rapidly secreted into the medium, which was not due to autolysis.
Similar content being viewed by others
References
Banuelos M, Gancedo C, Gancedi JM (1977) Activation by phosphate of yeast phosphofructokinase. J Biol Chem 252:6394–6398
Bernhauer K (1928) Z Physiol Chem 177:86 [cited in: Röhr et al. (1983b)]
Franke W, Eichhorn G, Möchel L, Bertram I (1963) Zur Kenntnis der sogenannten Glucose Oxidase. Zur Physiologie und Enzymatik der Gluconsäuregärung durch Aspergillus niger. Arch Microbiol 46:96–116
Gibson QH, Swoboda BEP, Massey V (1964) Kinetics and Mechanism of action of glucose oxidase. J Biol Chem 239:3927–3934
Kisser M, Kubicek CP, Röhr M (1980) Influence of manganese on morphology and cell wall composition of Aspergillus niger during citric acid fermentation. Arch Microbiol 128:26–33
Den Hollander JA, Ugurbil K, Brown TR, Shulman RG (1981) Phosphorus-31 nuclear magnetic resonance studies on the effect of oxygen upon glycolysis in yeast. Biochem 20:5871–5880
Kubicek CP, Pitt DE (1982) Effect of dilution rate on β-glucosidase secretion and cell wall metabolism in Trichoderma aureoviride cultivated in a continuous airlift fermenter. Eur J Appl Microbiol Biotechnol 16:189–196
Kubicek CP, Röhr M (1977) Influence of Manganese on enzyme synthesis and citric acid accumulation by Aspergillus niger. Eur J Appl Microbiol Biotechnol 4:167–173
Kusai K, Sekuzu I, Hagihara B, Okunuki K, Yamauchi S, Nakai M (1960) Crystallization of glucose oxidase from Penicillium amagasakiense. Biochim Biophys Acta 40:555–557
Lagos R, Ureta T (1980) The hexokinases from wild type and morphological mutant strains of Neurospora crassa. Eur J Biochem 104:357–365
Lockwood LB (1975) Organic acid production. In: Smith JE, Berry DR (eds) The filamentous fungi. Vol. I, Edward Arnold, London, p 140
Pazur JH (1966) Glucose oxidase from Aspergillus niger. In: Wood WA (ed) Methods in enzymology. Vol. 9, Academic Press, New York London, pp 82–86
Ma H, Kubicek CP, Röhr M (1981) Malate dehydrogenase isoenzymes in Aspergillus niger. FEMS Microbiol Letts 12:147–151
Pitt DE, Bull AT (1982) The use of autoradiography to quantify aspects of fungal growth and starvation in submerged liquid culture. Trans Br Mycol Soc 78:97–104
Röhr M, Salzbrunn WOJ, Stadler PJ, Kubicek CP (1979) An improved method for characterization of citrate production by conidia of Aspergillus niger. Biotechnol Letts 1:281–296
Röhr M, Zehentgruber O, Kubicek CP (1981) Kinetics of biomass formation and citric acid production by Aspergillus niger on a pilot plant scale. Biotechnol Bioengin 23:2422–2431
Röhr M, Kubicek CP, Kominek J (1983a) Citric acid. In: Rehm HJ, Reed G (eds) Biotechnology III Verlag Chemie, Weinheim, pp 420–454
Röhr M, Kubicek CP, Kominek J (1983b) Gluconic acid. In: Rehm HJ, Reed G (eds) Biotechnology III. Verlag Chemie, Weinheim, pp 456–465
Shu P, Johnson MJ (1947) Effect of the composition of the sporulation medium on citric acid production by Aspergillus niger in submerged culture. J Bacteriol 54:161–167
Van Dijken JP, Veenhuis M (1980) Cytochemical localization of glucose oxidase in peroxisomes of Aspergillus niger. Eur J Appl Microbiol Biotechnol 9:275–283
Zetelaki KZ (1970) The role of aeration and agitation in the production of glucose oxidase in submerged culture. Biotechnol Bioeng 12:379–397
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mischak, H., Kubicek, C.P. & Röhr, M. Formation and location of glucose oxidase in citric acid producing mycelia of Aspergillus niger . Appl Microbiol Biotechnol 21, 27–31 (1985). https://doi.org/10.1007/BF00252357
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00252357