Summary
The oligosaccharide-producing activity of β-galactosidase-1, one of the isomers of β-galactosidase (β-d-galactoside galactohydrolase, EC 3.2.1.23) from Bacillus circulans was changed after immobilization onto porous silica gel (Merckogel) by crosslinkage with glutaraldehyde. The reason for this modification was studied by treating the free enzyme with glutaraldehyde. Glutaraldehyde of 0.025% to 3% modified 40% to 90% of the free amino groups with or without intermolecular crosslinking. The maximum yield of oligosaccharides increased from 12% to 40% depending upon degree of modification, while native enzyme gave only 6% trisaccharides during hydrolysis of 127 mM lactose. The K m value for the enzyme treated with glutaraldehyde was also increased.
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Mozaffar, Z., Nakanishi, K. & Matsuno, R. Effect of glutaraldehyde on oligosaccharide production by β-galactosidase from Bacillus circulans . Appl Microbiol Biotechnol 25, 426–429 (1987). https://doi.org/10.1007/BF00253312
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DOI: https://doi.org/10.1007/BF00253312