Summary
A rapid assay for soil urease in the absence of bacteriostatic agents has been developed. The method comprises incubation of soil with an aqueous or buffered urea solution, extraction of ammonium with 1 N KCl and 0.01 NHCl and colorimetric NH4 + determination by a modified indophenol reaction. The method is characterized by high sensitivity and stability of the coloured complex formed. Measurements obtained by this method showed that no change in urease activity occurred when field-moist samples of soils were stored at −20°C for as long as 5 months. Air-drying of field-moist soil samples may lead to an increase in urease activity.
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References
Bonmati M, Pujola M, Sana J, Soliva M, Felipo MT, Garau M, Ceccanti B, Nannipieri P (1985) Chemical properties, populations of nitrite oxidizers, urease and phosphatase activities in sewage sludge amended soils. Plant Soil 84:79–91
Burns RG (1978) Soil enzymes. Academic Press, New York London, pp 1–340
Burns RG, Pukite AH, McLaren AD (1972) Concerning the location and persistence of soil urease. Soil Sci Soc Am Proc 36:308–311
Douglas LA, Bremner JM (1971) A rapid method of evaluating different compounds as inhibitors of urease in soils. Soil Biol Biochem 3:309–315
Galstyan ASH (1965) A method of determining the activity of hydrolytic enzymes in soil. Soviet Soil Sci 2:170–175
Gosewinkel U, Broadbent FE (1984) Conductimetric determination of soil urease activity. Commun Soil Sci Plant Anal 15:1377–1389
Gould LA, Cook FD, Webster GR (1973) Factors affecting urea hydrolysis in several Alberta soils. Plant Soil 38:393–401
Frankenberger JR, Johanson JB (1986) Use of plasmolytic agents and antiseptics in soil enzyme assays. Soil Biol Biochem 18:209–213
Hoffmann E, Schmidt W (1953) Über das Enzymsystem unserer Kulturböden. II Urease. Biochem Z 324:125–127
Hoffmann E, Teicher K (1961) Ein kolorimetrisches Verfahren zur Bestimmung der Ureaseaktivität in Böden. Z Pflanzenernachr Düngung Bodenkd 95:55–63
Hoffmann G, Dedeken M (1965) Eine Methode zur kolorimetrischen Bestimmung der Glucosidaseaktivität in Böden. Z Pflanzenernaehr Düngung Bodenkd 108:195–201
Kandeler E, Gerber H (1985) Ein einfaches Verfahren zur Bestimmung der Ureaseaktivität im Boden. VDLUFA-Schriftreihe, 16, Kongressband, pp 609–614
Kaplan DL, Hartenstein R (1979) Problems with toluene and the determination of extracellular enzyme activity in soils. Soil Biol Biochem 11:335–338
Keeney DR, Nelson DW (1982) Nitrogen-inorganic forms. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis, part 2. Madison, Wisc, Agronomy 9:643–698
Makboul HE, Ottow JCG (1979) Einflu\ von Zwei- und Dreischichttonmineralen auf die Dehydrogenase-, saure Phosphatase- und Urease-Aktivität in Modellversuchen. Z Pflanzenernaehr Bodenkd 142:500–513
McGarity JW, Myers MG (1967) A survey of urease activity in soils of northern New South Wales. Plant Soil 27:217–238
McIlvaine TC (1982) In: Metzner H (ed) Pflanzenphysiologische Versuche. Fischer, Stuttgart New York, pp 344
Nannipieri P, Johnson RL, Paul EA (1978) Criteria for measurement of microbial growth and activity in soil. Soil Biol Biochem 10:223–229
Nannipieri P, Pedrazzini F, Arcara PG, Piovanelli C (1979) Changes in amino acids, enzyme activities, and biomasses during soil microbial growth. Soil Sci 127:26–34
Nannipieri P, Muccini L, Ciardi C (1983) Microbial biomass and enzyme activities: Production and persistence. Soil Biol Biochem 15:679–685
Paulson KN, Kurtz LT (1969) Locus of urease activity in soil. Soil Sci Soc Am Proc 33:897–901
Pettit NM, Smith ARJ, Freedman RB, Burns RG (1976) Soil urease: Activity, stability and kinetic properties. Soil Biol Biochem 8:479–484
Skujins JJ, McLaren AD (1969) Assay of urease activity using 14C-urea in stored, geologically preserved, and in irradiated soils. Soil Biol Biochem 1:89–99
Tabatabai MA, Bremner JM (1972) Assay of urease activity in soils. Soil Biol Biochem 4:479–487
Thente B (1970) Effects of toluene and high energy radiation on urease activity in soil. Lantbrukshoegsk Ann 36:401–418
Thun R, Herrmann R (eds) (1949) Die Untersuchung von Boden. Neumann, Radebeul Berlin, pp 15–28
Wagner R (1969) Neue Aspekte zur Stickstoffanalytik in der Wasserchemie. Jahrbuch Vom Wasser 36:263–318
Walkley A, Black IA (1934) An examination of the Degtjareff method for determining soil organic matter and a proposed modifikation of chromic titration method. Soil Sci 37:29–38
Zantua MI, Bremner JM (1975a) Comparison of methods of assaying urease activity in soils. Soil Biol Biochem 7:291–295
Zantua MI, Bremner JM (1975b) Preservation of soil samples for assay of urease activity. Soil Biol Biochem 7:297–299
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Kandeler, E., Gerber, H. Short-term assay of soil urease activity using colorimetric determination of ammonium. Biol Fert Soils 6, 68–72 (1988). https://doi.org/10.1007/BF00257924
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DOI: https://doi.org/10.1007/BF00257924