Summary
The highest production of extracellular cellulases (Filter paper activity, Carboxymethyl cellulase and β-glucosidase) by Trichoderma harzianum was achieved on delignified wheat straw. Glycerol at a concentration of one per cent strongly repressed the formation of all the cellulolytic components. Addition of cyclic AMP did not relieve the repression caused by glycerol but lowered the level of existing cellulase enzymes.
References
Berghem L E R, Pettersson L G (1974) The mechanism of enzymatic cellulose degradation. Isolation and some properties of a β-glucosidase from Trichoderma viride. Eur J Biochem 41: 295–305
Chaudhary K, Tauro P (1982) Selective induction of β-glucosidase in Trichoderma reesei by xylan. Eur J Appl Microbiol Biotechnol 16: 185–187
Eriksson K E (1979) Biosynthesis of polysaccharases. In: Berkeley R C W, Gooday G W, Ellwood D C (eds) Microbial polysaccharides and polysaccharases. Academic Press, New York, pp 285–293
Halliwell G (1979) Microbial β-glucanases. Prog Indust Microbiol 15: 1–60
Holzer H (1976) Catabolite inactivation in yeast. TIBS (August) 178–181
Horton J C, Keen N T (1966) Regulation of induced cellulase synthesis in Pyrenochaete terrestris by utilizable carbon compounds Can J Microbiol 12: 209–220
Makonnen B, Porath J (1968) Purification of an extracellular proteinase from Penicillium notatum. Eur J Biochem 6: 425–431
Sandhu D K, Kalra M K (1982) Production of cellulase, xylanase and pectinase by Trichoderma longibrachiatum on different substrates. Trans Br Mycol Soc 79: 409–413
Sidhu M S, Sandhu D K (1980) Single cell protein production by Trichoderma longibrachiatum on treated sugar-cane bagasse. Biotechnol Bioeng 22: 689–692
Vaheri M P, Vaheri M E O, Kaupinen V S (1979) Formation and release of cellulolytic enzymes during growth of Trichoderma reesei on cellobiose and glycerol. Eur J Appl Microbiol Biotechnol 8: 73–80
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kalra, M.K., Sidhu, M.S., Sandhu, D.K. et al. Production and regulation of cellulases in Trichoderma harzianum . Appl Microbiol Biotechnol 20, 427–429 (1984). https://doi.org/10.1007/BF00261948
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00261948