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A new chemically-defined medium for RAC-certified and other strains of Bacillus subtilis

  • Applied Genetics and Regulation
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Summary

Conventional chemically-defined media were found to be inadequate for extensive batch culture growth of an asporogenous mutant of Bacillus subtilis certified as an HV-1 host for recombinant DNA. We developed a chemically-defined medium for rapid and extensive growth of one such strain, BGSC-1S53. The medium contains no components requiring separate sterilization. It is also useful for the prototrophic 168 strain (from which 1S53 was derived), a triple auxotroph of 168, and a highly debilitated (HV-2) 168 strain (BGSC 1S76) upon addition of the specific growth factors required by these strains. Excellent growth was also obtained with W-23, a Bacillus subtilis strain unrelated to the 168 series.

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References

  • Anagnostopoulos C, Spezizen J (1961) Requirements for transformation in Bacillus subtilis. J Bacteriol 81:741–746

    Google Scholar 

  • Armstrong RL, Hartford N, Kennett RH, St. Pierre ML, Sueoka N (1970) Experimental methods for Bacillus subtilis. In: Tabor H, Tabor CW (eds) Methods in enzymology, Vol 17A, Academic Press, New York pp 36–59

    Google Scholar 

  • Burke WF Jr, Le HT (1980) Characterization of a Bacillus subtilis strain. Recomb DNA Tech Bull 3:175–194

    Google Scholar 

  • Demain AL (1958) Minimal media for quantitative studies with Bacillus subtilis. J Bacteriol 75:517–522

    Google Scholar 

  • Deshpande KL, Katze JR, Kane JF (1980) Regulation of glutamate synthase from Bacillus subtilis by glutamine. Biochem Biophys Res Commun 95:55–60

    Google Scholar 

  • Dubnau D, Gryczan T, Contente S, Shirakumar AG (1980) Molecular cloning in Bacillus subtilis. In: Setlow JK, Hollaender A (eds) Genetic engineering: Principles and methods, Vol 2, Plenum Press, New York, pp 115–131

    Google Scholar 

  • Ellis DM, Dean DH (1981) Characterization of a non-reverting asporogenous strain of Bacillus subtilis 168 for use as an HV1 host. Recomb DNA Tech Bull 4:1–3

    Google Scholar 

  • Young FE (1980) Impact of cloning in Bacillus subtilis on fundamental and industrial microbiology. J Gen Microbiol 119:1–15

    Google Scholar 

  • Young FE, Smith C, Reilly BE (1969) Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis. J Bacteriol 98:1087–1097

    Google Scholar 

  • Young FE, Wilson GA (1974) Bacillus subtilis. In: King RC (ed) Handbook of genetics, Vol 1, Plenum Press, New York, pp 69–114

    Google Scholar 

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Author notes

  1. AiQi Fang

    Present address: Institute of Microbiology, Yunnan University, Kunming, Yunnan, The People's Republic of China

Authors and Affiliations

  1. Fermentation Microbiology Laboratory, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts, USA

    AiQi Fang & Arnold L. Demain

Authors
  1. AiQi Fang
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  2. Arnold L. Demain
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Fang, A., Demain, A.L. A new chemically-defined medium for RAC-certified and other strains of Bacillus subtilis . Appl Microbiol Biotechnol 30, 144–147 (1989). https://doi.org/10.1007/BF00264002

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  • DOI: https://doi.org/10.1007/BF00264002

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