Summary
Proteins were extracted from rat liver ribosomes and ribosomal subunits: with 67% acetic acid (in the presence of 3.3 mM, 33 mM, or 67 mM Mg++); with 2 M LiCl in 4 M urea; with 0.25 N HCl; with 1% SDS; and after RNase digestion. The most efficient extraction and the best recovery were either with acetic acid in the presence of 33 mM or 67 mM Mg++, or with LiCl-urea. Protein extracted with acetic acid, LiCl-urea, or with HCl had little or no contamination with RNA. The ribosomal proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis: the proteins extracted with acetic acid were the most soluble in the sample gel solution; their electrophoretograms displayed the maximum number of spots and the smallest number of derivatives or altered proteins. Preparations of protein extracted with SDS or RNase were relatively insoluble in the sample gel solution, and proteins extracted with HCl showed a large number of derivatives. All things considered, the most satisfactory method for the extraction of protein from eukaryotic ribosomes is with 67% acetic acid in the presence of 33 mM MgCl2.
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Communicated by H. G. Wittmann
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Sherton, C.C., Wool, I.G. The extraction of proteins from eukaryotic ribosomes and ribosomal subunits. Molec. Gen. Genet. 135, 97–112 (1974). https://doi.org/10.1007/BF00264778
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DOI: https://doi.org/10.1007/BF00264778