Summary
Mutagenesis by 5-bromouracil of lambda phage to clear plaque formers does not depend on the recA function of the host E. coli cell or on the red function of the phage. Pretreatment of the host cells with ultraviolet light does not affect bromouracil mutagenesis of the adsorbed phage. Mutagenesis by hydroxlamine to clear plaque formers takes place at a high level in recA - host cells, and is not changed by preirradiation of rec + (wild type) hosts with ultraviolet light. Thus, bromouracil and hydroxylamine appear to mutate λ phage by a process which differs from that responsible for ultraviolet mutagenesis.
Two characteristics of bromouracil mutagenesis —the nonlinear dependence of the number of mutants on bromouracil incorporation, and a high frequency of heterozygotes — fit in with Rydberg's (1977) picture of bromouracil mutagenesis as a consequence of base mispairing, with mismatch repair removing the mutations at low incorporation of the analog.
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References
Budowsky, E.I.: The mechanism of the mutagenic action of hydroxylamines. Prog. Nucleic Acid. Res. Mol. Biol. 16, 125–188 (1976)
Cohen, S.N., Chang, A.C.Y.: Genetic expression in bacteriophage lambda III: Inhibition of E. coli nucleic acid and protein synthesis during lambda development. J. molec. Biol. 49, 557–575 (1970)
DeFais, M., Fauquet, P., Radman, M., Errera, M.: Ultraviolet inactivation and ultraviolet mutagenesis of lambda in different genetic systems. Virology 43, 495–503 (1971)
Drake, J.W.: The molecular basis of mutation. Holden-Day, San Francisco 1970
Manly, K.F., Signer, K.F., Radding, C.M.: Nonessential functions of bacteriophage lambda. Virology 37, 177–188 (1969)
Parkinson, J.S.: Genetics of the left arm of the chromosome of bacteriophage lambda. Genetics 59, 311–325 (1968)
Pietrzykowska, I.: On the mechanism of bromouracil-induced mutagenesis. Mutation Res. 19, 1–9 (1973)
Radman, M.: SOS repair hypothesis: Phenomenology of an inductible DNA repair which is accompanied by mutagenesis. In: Molecular mechanisms for repair of DNA, Part A, pp. 355–367 (Hanawalt, P.C. and Setlow, R.B., eds.). New York: Plenum Press 1975
Ronen, A., Rahat, A.: Mutagen specificity and position effects on mutation in T4rII nonsense sites. Mutation Res. 34, 21–34 (1976)
Rydberg, B.: Bromouracil mutagenesis in E. coli: Evidence for involvement of mismatch repair. Molec. gen. Genet. 152, 19–28 (1977)
To, C.M., Kellenberger, E., Eisenstark, A.: Disassembly of T-even bacteriophage into structural parts and subunits. J. molec. Biol. 46, 493–511 (1969)
Walton, M.F., Cumming, R.B.: 5-halogenated uracil base anlog mutagenesis. Seventh Annual Meeting, Environmental Mutagen Soc., Atlanta, Ga. March, 1976
Weigle, J.: Induction of mutations in a bacterial virus. Proc. nat. Acad. Sci. (Wash.) 39, 628–636 (1953)
Witkin, E.M.: Mutation-proof and mutation-prone modes of survival in derivatives of E. coli B differing in sensitivity to ultraviolet light. Brookhaven Symp. Biol. 20, 17–53 (1967)
Witkin, E.M., Parisi, E.C.: Bromouracil mutagenesis: Mispairing or misrepair. Mutation Res. 25, 407–409 (1974)
Zissler, J., Signer, E., Schaefer, F.: The role of recombination in growth of bacteriophage lambda I: The gamma gene. In: The bacteriophage lambda (Hershey, A.D., ed.), pp. 455–468. New York: Cold Spring Harbor Laboratory 1971a
Zissler, J., Signer, E., Schaefer, F.: The role of recombination in growth of bacteriophage lambda II. Inhibition of growth by prophage P2. In: The bacteriophage lambda (Hershey, A.D., ed.), pp. 469–476. New York: Cold Spring Harbor Laboratory 1971b
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Hutchinson, F., Stein, J. Mutagenesis of lambda phage: 5-bromouracil and hydroxylamine. Molec. Gen. Genet. 152, 29–36 (1977). https://doi.org/10.1007/BF00264936
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DOI: https://doi.org/10.1007/BF00264936