Summary
Lipids were extracted from the diploid seed and haploid pollen of Brassica napus L. Two fractions of pollen lipids, namely the diploid-specified pollen-coat and the haploid-specified internal cytoplasmic lipids were obtained. Significant correlations exist between pollen and seed generations for linoleic (18∶2) and linolenic (18∶3) acids. In pollen internal storage lipids, the level of 18∶3 is positively correlated and the level of 18∶2 is negatively correlated with the level of 18∶3 in seed lipids. Evidence is presented that strongly supports the hypothesis that lipid biosynthesis occurs within the pollen and that synthesis is specified by haploid genes. These data support the concept of pollen selection, so that selecting among living pollen grains for superior individuals has potential as a new plant breeding tool for improving seed oil quality.
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References
Ackman RG (1983) Chemical composition of rapeseed oil. In: Kramer JKG, Sauer FD, Pisden WJ (eds) High and low erucic acid rapeseed oils. Academic Press, Toronto, pp 85–129
Bird JM, Porter EK, Dickinson HG (1982) Events in the cytoplasm during male mitosis in Lilium henryii. J Cell Sci 59:27–42
Brink RA, MacGilivray JH (1924) Segregation for the waxy character in maize pollen and differential development of the male gametophyte. Am J Bot 11:465–469
Browse J, Warwick N, Somerville CR, Slack CR (1986) Fluxes through the prokaryotic and eukaryotic pathways of lipid synthesis in the “16∶3” plant Arabidopsis thaliana. Biochem J 235:25–31
Christie WW (1982) Lipid analysis, 2nd edn. Pergamon Press, Sydney
Cresti M, Ciampolini F, Kapil RN (1983) Ultrastructure of S3S4 genotype pollen grains of Oenothera organensis. Acta Bot Neerl 32:177–183
Demerec M (1924) A case of pollen dimorphism in maize. Am J Bot 11:461–464
Dickinson HG (1973) The role of plastids in the formation of pollen grain coatings. Cytobios 8:25–40
Dickinson HG, Bell PR (1976) Development of the tapetum in Pinus banksiana proceeding sporogenesis. Ann. Bot 40:103–113
Dickinson HG, Heslop-Harrison J (1970) The behavior of plastids during meiosis in microsporocytes of Lilium longiflorum Thumb. Cytobiosis 2:103–118
Dickinson HG, Heslop-Harrison J (1977) Ribosomes, membranes and organelles during meiosis in angiosperms. Philos Trans R Soc London 277:327–342
Dickinson HG, Lewis D (1973) The formation of the ‘thyphine’ coating the pollen grains of Raphanus and its properties relating to self incompatibility. Proc R Soc London Ser B 184:149–165
Douce R, Joyard J (1980) Chloroplast envelope lipids: Detection and Biosynthesis. Methods Enzymol 69:290–301
Downey RK (1978) Breeding for quality. Int Rapeseed Conf Proc 1:106–112
Downey RK, Craig BM (1964) Genetic control of fatty acid biosynthesis in rapeseed (Brassica napus L.). J Am Oil Chem Soc 41:475–478
Dumas C, Duplan JC, Said C, Soulier JP (1983) ‘H Nuclear magnetic resonance to correlate water content and pollen viability. In: Mulcahy DL, Ottaviano E (eds) Pollen biology and implications of plant breeding. Elsevier, New York Amsterdam Oxford, pp 15–20
Dungey SG, Sang JP, Rothnie NE, Palmer MV, Burke DG, Knox RB, Williams EG, Hilliard EP, Salisbury PA (1988) Glucosinolates in the pollen of rapeseed and Indian mustard. Phytochemistry 27:815–817
Evans DE, Rothnie NE, Palmer MV, Burke DG, Sang JP, Knox RB, Williams EG, Hilliard EP, Salisbury PA (1987) Comparative analysis of fatty acids in pollen and seed of rapeseed. Phytochemistry 26(7):1895–1898
Fowler DB, Downey RK (1970) Lipid and morphological changes in developing rapeseed Brassica napus. Can J Plant Sci 50:233–247
Gabara B (1977) Radioautographic visualization of incorporation of lipid precursors into anthers of Muscari comosum (L.) Mill. Acta Soc Bot Pol 36:295–302
Gaude T, Dumas C (1984) A membrane-like structure on the pollen wall surface of Brassica. Ann Bot 54:821–825
Gay G, Herhoas C, Dumas C (1986) Pollen gene expression analysed by micro-isoelectric focusing of proteins from isolated pollen grains in Cucurbita pepo L. Theor Appl Genet 23:47–52
Green AG, Marshall DR (1984) Isolation of induced mutants in linseed (Linum usitatissimum) having reduced linolenic acid content. Euphytica 33:321–328
Heslop-Harrison J (1968) Tapetal origin of pollen-coat substances in Lilium. New Phytol 67:779–786
Iwanami Y, Nakamura N (1972) Storage in an organic solvent as a means for preserving viability of pollen grains. Stain Technol 47:137–139
Knox RB, Dickinson HG, Heslop-Harrison J (1970) Cytochemical observations on changes in RNA content and acid phosphatase activity during meiotic prophase in the culture of Cosmos bipinnatus Cav. Acta Bot Neerl 19:1–6
Kyo M, Harada H (1986) Control of the developmental pathway of tobacco pollen in vitro. Planta 168:427–432
Mackenzie A, Heslop-Harrison J, Dickinson HG (1967) Cytology elimination of ribosomes during meiotic prophase. Nature 215:997–999
Mascarenhas JP (1975) The biochemistry of angiosperm development. Bot Rev 41:259–314
Mascarenhas JP, Stinson JS, Willing RP, Pe ME (1985) Genes and their expression in the male gametophyte of flowering plants. In: Mulcahy DL, Mulcahy GB, Ottaviano E (eds) Biotechnology and ecology of pollen. Springer, Berlin Heidelberg New York, pp 39–44
Moss GI, Heslop-Harrison J (1967) A cytochemical study of DNA, RNA and protein in the developing maize anther. II. Observations. Ann Bot 31:555–572
Mulcahy DL, Mulcahy GB, Robinson RW (1979) Evidence for postmeiotic genetic activity in pollen of Cucurbita species. J Hered 70:365–368
Ottaviano E, Sari-Gorla M, Mulcahy DL (1980) Pollen tube growth rates in Zea mays: Implications for genetic improvement of crops. Science 210:437–438
Parnell FR (1921) Note on the detection of segregation by examination of the pollen of rice. J Genet 11:209–212
Porter EK, Parry D, Dickinson HG (1983) Changes in poly (A)+ RNA during male meiosis in Lilium. J Cell Sci 62:177–186
Reynolds TL, Raghavan V (1982) An autoradiographic study of RNA synthesis during maturation and germination of pollen grains of Hyocyamus niger. Protoplasma 111:177–188
Reznickova SA, Dickinson HG (1982) Ultrastructural aspects of storage lipid mobilization in the tapetum of Lilium hybrida var. enchantment. Planta 155:400–408
Reznickova SA, Willemse MTM (1981a) Electron microscopic and histochemical investigation of tissues of the developing lily anther in connection with metabolism of reserve nutrient substances. Sov Plant Physiol 28:856–864
Reznickova SA, Willemse MTM (1981b) The function of the tapetal tissue during microsporogenesis in Lilium. Acta Soc Bot Pol 50:83–87
Roy NN, Tarr AW (1985) IXLIN ⊕n interspecific source for high linoleic and low linolenic acid content in rapeseed (B. napus L.) Z Pflanzenzücht 95:201–209
Sacher RF, Mulcahy DL, Staples RC (1983) Developmental selection during self pollination of Lycopersicon x Solanum F1 for salt tolerance of F2. In Mulcahy DL, Ottaviano E (eds) Pollen: biology and implications for plant breeding. Elsevier, New York Amsterdam Oxford, pp 329–342
Sari Gorla M, Frova C, Ottaviano E, Soave C (1983) Gene expression at the gametophytic phase in maize. In: Mulcahy DL, Ottaviano E (eds) Pollen: Biology and implications for plant breeding. Elsevier, Amsterdam, pp 323–328
Schwartz D, Osterman J (1976) A pollen selection system for alcohol-dehydrogenase negative mutants in plants. Genetics 83:63–65
Searcy KB, Mulcahy DL (1985) Pollen selection and the gametophytic expression of metal tolerance in Silene dioica (Caryophyllaceae) and Mimulus guttatus (Scrophulariaceae). Am J Bot 72:1700–1706
Simon CJ, Sanford JC (1985) Induction of gametic selection in situ by stylar application of selective agents. In: Mulcahy DL, Bergamiki Mulcahy G, Ottaviano E (eds) Biotechnology and ecology of pollen. Springer, Berlin Heidelberg New York, pp 107–113
Singh MB, Knox RB (1984) Quantitative cytochemistry of β-galactosidase in normal and enzyme deficient (gal) pollen of Brassica campestris: application of the indigogenic method. Histochem J 16:1273–1296
Slack CR (1983) Phosphatidylcholine, an intermediate in polyunsaturated triacylglycerol synthesis. In: Thomson WW, Mudd JB, Gibbs M (eds) Biosynthesis and function of plant lipids. Waverly Press, Baltimore, Maryland
Smith G (1985) Sporophytic screening and gametophytic verification of phytotoxin tolerance in sugarbeet (Beta vulgaris L.) In: Mulcahy DL, Bergamiki Mulcahy G, Ottaviano E (eds) Biotechnology and ecology of pollen. Springer, Berlin Heidelberg New York, pp 83–88
Stobart AK, Styme S (1985) The regulation of the fatty acid composition of triacylglycerols in microsomal preparations from avacado mesocarp and the developing cotyledons of safflower. Planta 163:119–125
Stobart AK, Styme S, Glad G (1983) The synthesis of linoleate and phosphatidic acid and its relationship to oil production in the microsomes of developing seeds of safflower (Carthamu trictorius L. var Gila). Biochem Biophys Acta 754:292–297
Taylor JH, McMaster RD (1954) Autoradiographic and microphotometric studies of DNA during microgametogenesis in Lilium longiflorum. Chromosoma 6:489–521
Thompson GA, Roughan PG, Browse JA, Slack CR, Gardiner SE (1986) Spinach leaves desaturate exogenous (14C) Palmitate to Hexadecatrienate. Plant Physiol 82:357–362
Tupy J (1982) Alterations in polyadenylated RNA during pollen maturation and germination. Biol Plant 25:331–340
Tupy J, Suss J, Rihova L (1986) RNA synthesis and ribosome status in pollen tube growth of Nicotiana tabacum L.; effects of external pH. J Plant Physiol 123:467–476
Vasil IK (1967) Physiology and cytology of anther development. Biol Rev 42:327–373
Vles RO (1974) Nutritional aspects of rapeseed oils. In: Proc 4th Int Rapeseed Conf, Giessen pp 17–30
Weeden NF, Gottlieb LD (1979) Distinguishing allozymes and isozymes of phosphoglucoisomerases by electrophoretic comparisons of pollen and somatic tissues. Biochem Genet 17:287–296
Whipple AP, Mascarenhas JP (1978) Lipid synthesis in germinating Tradescantia pollen. Phytochemistry 17:1273–1274
Whitaker BD (1986) Fatty acid composition of polar lipids in fruit and leaf chloroplasts “16∶3” and “18∶3” plant species. Planta 169:313–319
Williams JP, Kahn MU, Mitchell K (1983) Galactolipid biosynthesis in leaves of 16∶3 and 18∶3 plants. In: Thompson WW, Mudd JB, Gibbs M (eds) Biosynthesis and function of plant lipids. Waverly Press, Baltimore, Maryland, pp 28–39
Willing RP, Mascarenhas JP (1984) Analysis of the complexity and diversity of mRNAs from pollen and shoots of Tradescantia. Plant Physiol 75:865:868
Winsor JA, Davis LE, Slephenson AG (1987) The relationship between pollen load and fruit maturation and the effect of pollen load on offspring vigour in Cucurbita pepo. Am Nat 129:643–656
Zamir D, Tanksley SD, Jones RA (1982) Haploid selection for low temperature tolerance of tomato pollen. Genetics 101:129–137
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Communicated by H. F. Linskens
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Evans, D.E., Rothnie, N.E., Sang, J.P. et al. Correlations between gametophytic (pollen) and sporophytic (seed) generations for polyunsaturated fatty acids in oilseed rape Brassica napus L.. Theoret. Appl. Genetics 76, 411–419 (1988). https://doi.org/10.1007/BF00265342
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DOI: https://doi.org/10.1007/BF00265342